Autotoxicity stress is the principal factor in peach replant problem.Benzoic acid(BA)is known as a critical autotoxin in replant problem,and causes an obvious inhibitory effect on peach growth.Small heat shock protein...Autotoxicity stress is the principal factor in peach replant problem.Benzoic acid(BA)is known as a critical autotoxin in replant problem,and causes an obvious inhibitory effect on peach growth.Small heat shock proteins(sHSPs)have been reported to play pivotal roles in a variety of physiological and biological processes in various plants.Nevertheless,little is known about the functions and the underlying physiological mechanisms of sHSPs under autotoxicity stress.Here,we identified PpHSP20-26 of peach(Prunus persica)and deciphered its role in BA stress response.PpHSP20-26 was significantly induced by BA treatment.Overexpression of PpHSP20-26 elevated BA tolerance in Arabidopsis and peach plants,whereas down-regulation of PpHSP20-26 in peach through virus-induced gene silencing enhanced BA sensitivity.Compared to the control,the PpHSP20-26-overexpressing plants exhibited lower contents of reactive oxygen species(ROS)and higher activities of antioxidant enzymes.Furthermore,PpHSP20-26 regulated the transcripts of stress-responsive genes including CAT,SOD,APX,GPX,DHAR,and ABC transporters in overexpressing Arabidopsis and silenced peach plants.Taken together,these data suggest that PpHSP20-26 plays a positive role in peach response to BA stress by,at least partly,regulating ROS metabolism and stress-responsive gene expression.Our findings will be of great importance for further understanding the roles of sHSPs genes in autotoxicity stress,and assist crop breeding in mitigating replant problem.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.32272645)China Agriculture Research System of MOF and MARA(Grant No.CARS-30)Changsha Municipal Natural Science Foundation(Grant No.kq2208135).
文摘Autotoxicity stress is the principal factor in peach replant problem.Benzoic acid(BA)is known as a critical autotoxin in replant problem,and causes an obvious inhibitory effect on peach growth.Small heat shock proteins(sHSPs)have been reported to play pivotal roles in a variety of physiological and biological processes in various plants.Nevertheless,little is known about the functions and the underlying physiological mechanisms of sHSPs under autotoxicity stress.Here,we identified PpHSP20-26 of peach(Prunus persica)and deciphered its role in BA stress response.PpHSP20-26 was significantly induced by BA treatment.Overexpression of PpHSP20-26 elevated BA tolerance in Arabidopsis and peach plants,whereas down-regulation of PpHSP20-26 in peach through virus-induced gene silencing enhanced BA sensitivity.Compared to the control,the PpHSP20-26-overexpressing plants exhibited lower contents of reactive oxygen species(ROS)and higher activities of antioxidant enzymes.Furthermore,PpHSP20-26 regulated the transcripts of stress-responsive genes including CAT,SOD,APX,GPX,DHAR,and ABC transporters in overexpressing Arabidopsis and silenced peach plants.Taken together,these data suggest that PpHSP20-26 plays a positive role in peach response to BA stress by,at least partly,regulating ROS metabolism and stress-responsive gene expression.Our findings will be of great importance for further understanding the roles of sHSPs genes in autotoxicity stress,and assist crop breeding in mitigating replant problem.
