Background:Hua-Yi-Jie-Du formula(HYJD)is a traditional Chinese medicine that has proven effective against viral pneumonia and was extensively used during the COVID-19 pandemic.This study investigates how HYJD influenc...Background:Hua-Yi-Jie-Du formula(HYJD)is a traditional Chinese medicine that has proven effective against viral pneumonia and was extensively used during the COVID-19 pandemic.This study investigates how HYJD influences group 2 innate lymphoid cell(ILC2)and nucleotide oligomerization domain(NOD)-like receptor protein 3(NLRP3)inflammasome activation in a mouse model of viral pneumonia.Methods:A mouse model of viral pneumonia was established through the administration of polyinosinic-polycytidylic acid(poly(I:C))via nasal drops.Histopathological analysis of lung tissue was conducted,alongside enzyme-linked immunosorbent assay to quantify cytokine levels in serum and bronchoalveolar lavage fluid(BALF).Flow cytometry was employed to detect ILC2 cells in lung tissue and spleen,while immunofluorescence techniques were utilized to visualize ILC2 cells in lung tissue.Transcriptomic sequencing was performed,and the results were validated using qRT-PCR and western blot analysis.Results:HYJD significantly ameliorated inflammatory infiltration in lung tissue,decreased mucus protein secretion,and reduced the serum levels of inflammatory cytokines interleukin(IL)-1β,IL-6,and tumor necrosis factor-alpha(TNF-α).Additionally,it lowered the expression of cytokines IL-4,IL-5,IL-13,IL-25,thymic stromal lymphopoietin(TSLP),and IL-33 in BALF,and reduced the differentiation of ILC2 cells in both lung tissue and spleen.Transcriptomic analysis and experimental validation revealed that HYJD downregulated the expression of NLRP3 related genes and proteins within the NOD-like receptor signaling pathway.Conclusion:The mechanism by which HYJD intervenes in acute lung injury associated with viral pneumonia may involve the reduction of ILC2 cells differentiation and the inhibition of NLRP3 activation.展开更多
The characteristics of the waves around a lagoon entrance in South China Sea were observed during the past one and half years based on the wave measurements.The measured results show that the monsoon plays an importan...The characteristics of the waves around a lagoon entrance in South China Sea were observed during the past one and half years based on the wave measurements.The measured results show that the monsoon plays an important role.In summer,waves mostly come from the southeast,among which the wave components of non-dimensional periods larger than 0.2 are fetch-trapped waves.In winter the waves may be divided into two groups,the fetch-trapped waves and the waves from the open sea.The skewness of the directional distribution functions is within+8 around zero.The power relationship between the direction spread and kurtosis is also investigated.Besides,it is found that the waves with the significant wave heights greater than 1.5 m are caused by typhoons or cold air.The frequency components of wave energy driven by cold air appear in the high frequency range at first,and change to the lower frequency range afterwards.The waves driven by typhoon mostly come from the open sea.展开更多
基金supported by Yunnan Provincial Major Science and Technology Special Program(No.202402AA310035)Yunnan Key Laboratory of Dai and Yi Medicines(Yunnan University of Chinese Medicine)(No.2024JS2404)Research Foundation of Chuxiong Medical College(No.2024YYXM38).
文摘Background:Hua-Yi-Jie-Du formula(HYJD)is a traditional Chinese medicine that has proven effective against viral pneumonia and was extensively used during the COVID-19 pandemic.This study investigates how HYJD influences group 2 innate lymphoid cell(ILC2)and nucleotide oligomerization domain(NOD)-like receptor protein 3(NLRP3)inflammasome activation in a mouse model of viral pneumonia.Methods:A mouse model of viral pneumonia was established through the administration of polyinosinic-polycytidylic acid(poly(I:C))via nasal drops.Histopathological analysis of lung tissue was conducted,alongside enzyme-linked immunosorbent assay to quantify cytokine levels in serum and bronchoalveolar lavage fluid(BALF).Flow cytometry was employed to detect ILC2 cells in lung tissue and spleen,while immunofluorescence techniques were utilized to visualize ILC2 cells in lung tissue.Transcriptomic sequencing was performed,and the results were validated using qRT-PCR and western blot analysis.Results:HYJD significantly ameliorated inflammatory infiltration in lung tissue,decreased mucus protein secretion,and reduced the serum levels of inflammatory cytokines interleukin(IL)-1β,IL-6,and tumor necrosis factor-alpha(TNF-α).Additionally,it lowered the expression of cytokines IL-4,IL-5,IL-13,IL-25,thymic stromal lymphopoietin(TSLP),and IL-33 in BALF,and reduced the differentiation of ILC2 cells in both lung tissue and spleen.Transcriptomic analysis and experimental validation revealed that HYJD downregulated the expression of NLRP3 related genes and proteins within the NOD-like receptor signaling pathway.Conclusion:The mechanism by which HYJD intervenes in acute lung injury associated with viral pneumonia may involve the reduction of ILC2 cells differentiation and the inhibition of NLRP3 activation.
基金supported by the Ministry of Industry and Information Technology(Grant Nos.[2016]22,[2019]357)supported by the Fund of Southern Marine Science and Engineering Guangdong Laboratory(Zhanjiang)(Grant Nos.ZJW-2019-02,ZJW-2019-08)the Jiangsu Province Science Foundation for Youths(Grant No.351510008K0708LA00).
文摘The characteristics of the waves around a lagoon entrance in South China Sea were observed during the past one and half years based on the wave measurements.The measured results show that the monsoon plays an important role.In summer,waves mostly come from the southeast,among which the wave components of non-dimensional periods larger than 0.2 are fetch-trapped waves.In winter the waves may be divided into two groups,the fetch-trapped waves and the waves from the open sea.The skewness of the directional distribution functions is within+8 around zero.The power relationship between the direction spread and kurtosis is also investigated.Besides,it is found that the waves with the significant wave heights greater than 1.5 m are caused by typhoons or cold air.The frequency components of wave energy driven by cold air appear in the high frequency range at first,and change to the lower frequency range afterwards.The waves driven by typhoon mostly come from the open sea.
