BACKGROUND Centipedes have been used to treat tumors for hundreds of years in China.However,current studies focus on antimicrobial and anticoagulation agents rather than tumors.The molecular identities of antihepatoma...BACKGROUND Centipedes have been used to treat tumors for hundreds of years in China.However,current studies focus on antimicrobial and anticoagulation agents rather than tumors.The molecular identities of antihepatoma bioactive components in centipedes have not yet been extensively investigated.It is a challenge to isolate and characterize the effective components of centipedes due to limited peptide purification technologies for animal-derived medicines.AIM To purify,characterize,and synthesize the bioactive components with the strongest antihepatoma activity from centipedes and determine the antihepatoma mechanism.METHODS An antihepatoma peptide(scolopentide)was isolated and identified from the centipede scolopendra subspinipes mutilans using a combination of enzymatic hydrolysis,a Sephadex G-25 column,and two steps of high-performance liquid chromatography(HPLC).Additionally,the CCK8 assay was used to select the extracted fraction with the strongest antihepatoma activity.The molecular weight of the extracted scolopentide was characterized by quadrupole time of flight mass spectrometry(QTOF MS),and the sequence was matched by using the Mascot search engine.Based on the sequence and molecular weight,scolopentide was synthesized using solid-phase peptide synthesis methods.The synthetic scolopentide was confirmed by MS and HPLC.The antineoplastic effect of extracted scolopentide was confirmed by CCK8 assay and morphological changes again in vitro.The antihepatoma effect of synthetic scolopentide was assessed by the CCK8 assay and Hoechst staining in vitro and tumor volume and tumor weight in vivo.In the tumor xenograft experiments,qualified model mice(male 5-week-old BALB/c nude mice)were randomly divided into 2 groups(n=6):The scolopentide group(0.15 mL/d,via intraperitoneal injection of synthetic scolopentide,500 mg/kg/d)and the vehicle group(0.15 mL/d,via intraperitoneal injection of normal saline).The mice were euthanized by cervical dislocation after 14 d of continuous treatment.Mechanistically,flow cytometry was conducted to evaluate the apoptosis rate of HepG2 cells after treatment with extracted scolopentide in vitro.A Hoechst staining assay was also used to observe apoptosis in HepG2 cells after treatment with synthetic scolopentide in vitro.CCK8 assays and morphological changes were used to compare the cytotoxicity of synthetic scolopentide to liver cancer cells and normal liver cells in vitro.Molecular docking was performed to clarify whether scolopentide tightly bound to death receptor 4(DR4)and DR5.qRT-PCR was used to measure the mRNA expression of DR4,DR5,fas-associated death domain protein(FADD),Caspase-8,Caspase-3,cytochrome c(Cyto-C),B-cell lymphoma-2(Bcl-2),Bcl-2-associated X protein(Bax),x-chromosome linked inhibitor-of-apoptosis protein and Cellular fas-associated death domain-like interleukin-1βconverting enzyme inhibitory protein in hepatocarcinoma subcutaneous xenograft tumors from mice.Western blot assays were used to measure the protein expression of DR4,DR5,FADD,Caspase-8,Caspase-3,and Cyto-C in the tumor tissues.The reactive oxygen species(ROS)of tumor tissues were tested.RESULTS In the process of purification,characterization and synthesis of scolopentide,the optimal enzymatic hydrolysis conditions(extract ratio:5.86%,IC_(50):0.310 mg/mL)were as follows:Trypsin at 0.1 g(300 U/g,centipede-trypsin ratio of 20:1),enzymolysis temperature of 46°C,and enzymolysis time of 4 h,which was superior to freeze-thawing with liquid nitrogen(IC_(50):3.07 mg/mL).A peptide with the strongest antihepatoma activity(scolopentide)was further purified through a Sephadex G-25 column(obtained A2)and two steps of HPLC(obtained B5 and C3).The molecular weight of the extracted scolopentide was 1018.997 Da,and the peptide sequence was RAQNHYCK,as characterized by QTOF MS and Mascot.Scolopentide was synthesized in vitro with a qualified molecular weight(1018.8 Da)and purity(98.014%),which was characterized by MS and HPLC.Extracted scolopentide still had an antineoplastic effect in vitro,which inhibited the proliferation of Eca-109(IC_(50):76.27μg/mL),HepG2(IC_(50):22.06μg/mL),and A549(IC_(50):35.13μg/mL)cells,especially HepG2 cells.Synthetic scolopentide inhibited the proliferation of HepG2 cells(treated 6,12,and 24 h)in a concentration-dependent manner in vitro,and the inhibitory effects were the strongest at 12 h(IC_(50):208.11μg/mL).Synthetic scolopentide also inhibited the tumor volume(Vehicle vs Scolopentide,P=0.0003)and weight(Vehicle vs Scolopentide,P=0.0022)in the tumor xenograft experiment.Mechanistically,flow cytometry suggested that the apoptosis ratios of HepG2 cells after treatment with extracted scolopentide were 5.01%(0μg/mL),12.13%(10μg/mL),16.52%(20μg/mL),and 23.20%(40μg/mL).Hoechst staining revealed apoptosis in HepG2 cells after treatment with synthetic scolopentide in vitro.The CCK8 assay and morphological changes indicated that synthetic scolopentide was cytotoxic and was significantly stronger in HepG2 cells than in L02 cells.Molecular docking suggested that scolopentide tightly bound to DR4 and DR5,and the binding free energies were-10.4 kcal/mol and-7.1 kcal/mol,respectively.In subcutaneous xenograft tumors from mice,quantitative real-time polymerase chain reaction and western blotting suggested that scolopentide activated DR4 and DR5 and induced apoptosis in SMMC-7721 Liver cancer cells by promoting the expression of FADD,caspase-8 and caspase-3 through a mitochondria-independent pathway.CONCLUSION Scolopentide,an antihepatoma peptide purified from centipedes,may inspire new antihepatoma agents.Scolopentide activates DR4 and DR5 and induces apoptosis in liver cancer cells through a mitochondria-independent pathway.展开更多
Nucleophosmin/B23 (NPM) is a universally expressed nucleolar phosphoprotein that participates in proliferation, apoptosis, ribosome assembly, and centrosome duplication; however, the role of NPM in cell cycle regulati...Nucleophosmin/B23 (NPM) is a universally expressed nucleolar phosphoprotein that participates in proliferation, apoptosis, ribosome assembly, and centrosome duplication; however, the role of NPM in cell cycle regulation is not well characterized. We investigated the mechanism by which NPM is involved in cell cycle regulation. NPM was knocked down using siRNA in HepG2 hepatoblastoma cells. NPM translocation following actinomycin D (ActD) treatment was investigated using immunofluorescent staining. Expression of NPM and other factors involved in cell cycle regulation was examined by Western blotting. Cell cycle distribution was measured using flow cytometry to detect 5-ethynyl-2′-deoxyuridine (EdU) incorporation. Cell proliferation was quantified by the MTT assay. Knockdown of NPM increased the percentage of HepG2 cells in S phase and led to decreased expression of P53 and P21Cip1/WAF1. S-phase arrest in HepG2 cells was significantly enhanced by ActD treatment. Furthermore, knockdown of NPM abrogated ActD-induced G2/M phase cell cycle arrest. Taken together, these data demonstrate that inhibition of NPM has a significant effect on the cell cycle.展开更多
Objective To analyze the differences in the correlation of tongue image indicators among patients with benign lung nodules and lung cancer.Methods From July 1;2020 to March 31;2022;clinical information of lung cancer ...Objective To analyze the differences in the correlation of tongue image indicators among patients with benign lung nodules and lung cancer.Methods From July 1;2020 to March 31;2022;clinical information of lung cancer patients and benign lung nodules patients was collected at the Oncology Department of Longhua Hos-pital Affiliated to Shanghai University of Traditional Chinese Medicine and the Physical Ex-amination Center of Shuguang Hospital Affiliated to Shanghai University of Traditional Chi-nese Medicine;respectively.We obtained tongue images from patients with benign lung nod-ules and lung cancer using the TFDA-1 digital tongue diagnosis instrument;and analyzed these images with the TDAS V2.0 software.The extracted indicators included color space pa-rameters in the Lab system for both the tongue body(TB)and tongue coating(TC)(TB/TC-L;TB/TC-a;and TB/TC-b);textural parameters[TB/TC-contrast(CON);TB/TC-angular second moment(ASM);TB/TC-entropy(ENT);and TB/TC-MEAN];as well as TC parameters(perAll and perPart).The bivariate correlation of TB and TC features was analyzed using Pearson’s or Spearman’s correlation analysis;and the overall correlation was analyzed using canonical correlation analysis(CCA).Results Samples from 307 patients with benign lung nodules and 276 lung cancer patients were included after excluding outliers and extreme values.Simple correlation analysis indi-cated that the correlation of TB-L with TC-L;TB-b with TC-b;and TB-b with perAll in lung cancer group was higher than that in benign nodules group.Moreover;the correlation of TB-a with TC-a;TB-a with perAll;and the texture parameters of the TB(TB-CON;TB-ASM;TB-ENT;and TB-MEAN)with the texture parameters of the TC(TC-CON;TC-ASM;TC-ENT;and TC-MEAN)in benign nodules group was higher than lung cancer group.CCA further demon-strated a strong correlation between the TB and TC parameters in lung cancer group;with the first and second pairs of typical variables in benign nodules and lung cancer groups indicat-ing correlation coefficients of 0.918 and 0.817(P<0.05);and 0.940 and 0.822(P<0.05);re-spectively.Conclusion Benign lung nodules and lung cancer patients exhibited differences in correla-tion in the L;a;and b values of the TB and TC;as well as the perAll value of the TC;and the texture parameters(TB/TC-CON;TB/TC-ASM;TB/TC-ENT;and TB/TC-MEAN)between the TB and TC.Additionally;there were differences in the overall correlation of the TB and TC be-tween the two groups.Objective tongue diagnosis indicators can effectively assist in the diag-nosis of benign lung nodules and lung cancer;thereby providing a scientific basis for the ear-ly detection;diagnosis;and treatment of lung cancer.展开更多
Objective To investigate the facial spectrum and color characteristics of patients with essen-tial hypertension post administering antihypertensive drugs,establish a classification and evaluation model based on the fa...Objective To investigate the facial spectrum and color characteristics of patients with essen-tial hypertension post administering antihypertensive drugs,establish a classification and evaluation model based on the facial colors of the enrolled patients,and perform in-depth analysis on the important characteristics of their facial spectrum.Methods From September 3,2018,to March 23,2024,participants with essential hyperten-sion(receiving antihypertensive medication treatment,hypertension group)and normal blood pressure(control group)were recruited from the Cardiology Department of Shanghai Hospital of Traditional Chinese Medicine,the Coronary Care Unit of Shanghai Tenth People's Hospital,the Physical Examination Center of Shuguang Hospital Affiliated to Shanghai Uni-versity of Traditional Chinese Medicine,and the Gaohang Community Health Service Center.This study employed the propensity score matching(PSM)method to reduce study partici-pants selection bias.Spectral information in the facial visible light spectrum of the subjects was collected using a flame spectrometer,and the spectral chromaticity values were calculat-ed using the equal-interval wavelength method.The study analyzed the differences in spec-tral reflectance across various facial regions,including the entire face,forehead,glabella,nose,jaw,left and right zygomatic regions,left and right cheek regions as well as differences in parameters within the Lab color space between the two subject groups.Feature selection was conducted using least absolute shrinkage and selection operator(LASSO)regression,fol-lowed by the application of various machine learning algorithms,including logistic regres-sion(LR),support vector machine(SVM),random forest(RF),Naïve Bayes(NB),and eX-treme Gradient Boosting(XGB).The reduced-dimensional dataset was split in a 7:3 ratio to establish a classification and assessment model for facial coloration related to primary hyper-tension.Additionally,model fusion techniques were applied to enhance the predictive power.The performance of the models was evaluated using metrics including the area under the curve(AUC)and accuracy.Shapley Additive exPlanations(SHAP)was used to interpret the outcomes of the models.Results A total of 114 participants were included in both hypertension and control groups.Reflectance analysis across the entire face and eight predefined areas revealed that the hypertensive group exhibited significantly higher reflectance of corresponding color light in the blue-violet region(P<0.05)and a lower reflectance in the red region(P<0.05)compared with control group.Analysis of Lab color space parameters across the entire face and eight predefined areas showed that hypertensive group had significantly lower a and b values than control group(P<0.05).LASSO regression analysis identified a total of 18 facial color features that were highly correlated with hypertension,including the a values of the chin and the right cheek,the reflectance at 380 nm and at 780 nm of the forehead.The results of the multi-mod-el classification showed that the RF classification model was the most effective,with an AUC of 0.74 and an accuracy of 0.77.The combined model of RF+LR+SVM outperformed a single model in their classification performance,achieving an AUC of 0.80 and an accuracy of 0.76.SHAP model visualization results indicated that the top three contributors to ideal prediction results based on the characteristics from the facial spectrum were the reflectance at 380 nm across the entire face and of the nose as well as the a value of the chin.Conclusion Within the same age group,patients with essential hypertension exhibited signif-icant and regular changes in facial color and facial spectral reflectance parameters after the administration of antihypertensive drugs.Furthermore,facial reflectance indicators,such as the overall reflectance at 380 nm and the a value of the chin,could offer valuable references for clinically assessing the drug efficacy and health status of patients with essential hyperten-sion.展开更多
Last year,the doubling of the A-share market was unmatched in global capital markets.Fu- eled by appreciation of the RMB,sectors like finance and real estate as well newly revitalized areas like non- ferrous metals,th...Last year,the doubling of the A-share market was unmatched in global capital markets.Fu- eled by appreciation of the RMB,sectors like finance and real estate as well newly revitalized areas like non- ferrous metals,the steel and chemical industries,drove market growth,creat- ing many millionaires in the process. In terms of pure performance,all of the 13 sectors classified by the CSRC had strong performance with growth展开更多
The National Development and Reform Commission of China (NDRC) released on August 27 a report on the first-half operation and the second-half expectation of the coal industry.The coal industry is predicted to have a c...The National Development and Reform Commission of China (NDRC) released on August 27 a report on the first-half operation and the second-half expectation of the coal industry.The coal industry is predicted to have a consecutive balance of sup- ply and demand this year with a less strained supply in the second half.展开更多
According to customs statistics, Chinese trade imports and exports totaled US$ 1,759.32 billion from January to October this year,representing an increase of 23.5 percent over the same period last year, and nearly equ...According to customs statistics, Chinese trade imports and exports totaled US$ 1,759.32 billion from January to October this year,representing an increase of 23.5 percent over the same period last year, and nearly equal to the total amount of imports and exports from all of last year.Exports were US$ 985.84展开更多
The'three most-wanted'goods,those seen as a symbol of wealth for Chinese families, has seen an evolution from specific products to general services with average values that first increased from RMB 100 to RMB ...The'three most-wanted'goods,those seen as a symbol of wealth for Chinese families, has seen an evolution from specific products to general services with average values that first increased from RMB 100 to RMB 10,000,and now have moved upwards of RMB 100,000.While this measure is not the most scientific,it reflects the great changes China has undergone,and also mirrors the transformation of Chinese lifestyle.展开更多
Aseries of events that occurred in October 2007,are rewrit- ing the history of China's en- ergy industry:November 3 an Energy Law Committee asked the public for suggestions on improvements;forty top experts came t...Aseries of events that occurred in October 2007,are rewrit- ing the history of China's en- ergy industry:November 3 an Energy Law Committee asked the public for suggestions on improvements;forty top experts came together to form the National Energy Expert Advisory Committee on November 6;and the National Oil Reserve Center(NORC)展开更多
The Office of the National Ener- gy Leading Group announced a draft of the upcoming energy law on December 3,2007,in an effort to collect feedback from the public and the media through the Internet up to the 1 of Febr...The Office of the National Ener- gy Leading Group announced a draft of the upcoming energy law on December 3,2007,in an effort to collect feedback from the public and the media through the Internet up to the 1 of February 2008.This marks the first time that the draft of a new energy law has been released to the public since research and study work began in 2006;the event also symbolizes a new phase for energy legislation.展开更多
基金Supported by the National Natural Science Foundation of China,No.U20A20408 and No.82074450Natural Science Foundation of Hunan Province,No.2020JJ4066 and No.2021JJ40405+1 种基金Key scientific research project of Hunan Education Department,No.21A0243Key project of academician workstation guidance project,No.21YSZQ007.
文摘BACKGROUND Centipedes have been used to treat tumors for hundreds of years in China.However,current studies focus on antimicrobial and anticoagulation agents rather than tumors.The molecular identities of antihepatoma bioactive components in centipedes have not yet been extensively investigated.It is a challenge to isolate and characterize the effective components of centipedes due to limited peptide purification technologies for animal-derived medicines.AIM To purify,characterize,and synthesize the bioactive components with the strongest antihepatoma activity from centipedes and determine the antihepatoma mechanism.METHODS An antihepatoma peptide(scolopentide)was isolated and identified from the centipede scolopendra subspinipes mutilans using a combination of enzymatic hydrolysis,a Sephadex G-25 column,and two steps of high-performance liquid chromatography(HPLC).Additionally,the CCK8 assay was used to select the extracted fraction with the strongest antihepatoma activity.The molecular weight of the extracted scolopentide was characterized by quadrupole time of flight mass spectrometry(QTOF MS),and the sequence was matched by using the Mascot search engine.Based on the sequence and molecular weight,scolopentide was synthesized using solid-phase peptide synthesis methods.The synthetic scolopentide was confirmed by MS and HPLC.The antineoplastic effect of extracted scolopentide was confirmed by CCK8 assay and morphological changes again in vitro.The antihepatoma effect of synthetic scolopentide was assessed by the CCK8 assay and Hoechst staining in vitro and tumor volume and tumor weight in vivo.In the tumor xenograft experiments,qualified model mice(male 5-week-old BALB/c nude mice)were randomly divided into 2 groups(n=6):The scolopentide group(0.15 mL/d,via intraperitoneal injection of synthetic scolopentide,500 mg/kg/d)and the vehicle group(0.15 mL/d,via intraperitoneal injection of normal saline).The mice were euthanized by cervical dislocation after 14 d of continuous treatment.Mechanistically,flow cytometry was conducted to evaluate the apoptosis rate of HepG2 cells after treatment with extracted scolopentide in vitro.A Hoechst staining assay was also used to observe apoptosis in HepG2 cells after treatment with synthetic scolopentide in vitro.CCK8 assays and morphological changes were used to compare the cytotoxicity of synthetic scolopentide to liver cancer cells and normal liver cells in vitro.Molecular docking was performed to clarify whether scolopentide tightly bound to death receptor 4(DR4)and DR5.qRT-PCR was used to measure the mRNA expression of DR4,DR5,fas-associated death domain protein(FADD),Caspase-8,Caspase-3,cytochrome c(Cyto-C),B-cell lymphoma-2(Bcl-2),Bcl-2-associated X protein(Bax),x-chromosome linked inhibitor-of-apoptosis protein and Cellular fas-associated death domain-like interleukin-1βconverting enzyme inhibitory protein in hepatocarcinoma subcutaneous xenograft tumors from mice.Western blot assays were used to measure the protein expression of DR4,DR5,FADD,Caspase-8,Caspase-3,and Cyto-C in the tumor tissues.The reactive oxygen species(ROS)of tumor tissues were tested.RESULTS In the process of purification,characterization and synthesis of scolopentide,the optimal enzymatic hydrolysis conditions(extract ratio:5.86%,IC_(50):0.310 mg/mL)were as follows:Trypsin at 0.1 g(300 U/g,centipede-trypsin ratio of 20:1),enzymolysis temperature of 46°C,and enzymolysis time of 4 h,which was superior to freeze-thawing with liquid nitrogen(IC_(50):3.07 mg/mL).A peptide with the strongest antihepatoma activity(scolopentide)was further purified through a Sephadex G-25 column(obtained A2)and two steps of HPLC(obtained B5 and C3).The molecular weight of the extracted scolopentide was 1018.997 Da,and the peptide sequence was RAQNHYCK,as characterized by QTOF MS and Mascot.Scolopentide was synthesized in vitro with a qualified molecular weight(1018.8 Da)and purity(98.014%),which was characterized by MS and HPLC.Extracted scolopentide still had an antineoplastic effect in vitro,which inhibited the proliferation of Eca-109(IC_(50):76.27μg/mL),HepG2(IC_(50):22.06μg/mL),and A549(IC_(50):35.13μg/mL)cells,especially HepG2 cells.Synthetic scolopentide inhibited the proliferation of HepG2 cells(treated 6,12,and 24 h)in a concentration-dependent manner in vitro,and the inhibitory effects were the strongest at 12 h(IC_(50):208.11μg/mL).Synthetic scolopentide also inhibited the tumor volume(Vehicle vs Scolopentide,P=0.0003)and weight(Vehicle vs Scolopentide,P=0.0022)in the tumor xenograft experiment.Mechanistically,flow cytometry suggested that the apoptosis ratios of HepG2 cells after treatment with extracted scolopentide were 5.01%(0μg/mL),12.13%(10μg/mL),16.52%(20μg/mL),and 23.20%(40μg/mL).Hoechst staining revealed apoptosis in HepG2 cells after treatment with synthetic scolopentide in vitro.The CCK8 assay and morphological changes indicated that synthetic scolopentide was cytotoxic and was significantly stronger in HepG2 cells than in L02 cells.Molecular docking suggested that scolopentide tightly bound to DR4 and DR5,and the binding free energies were-10.4 kcal/mol and-7.1 kcal/mol,respectively.In subcutaneous xenograft tumors from mice,quantitative real-time polymerase chain reaction and western blotting suggested that scolopentide activated DR4 and DR5 and induced apoptosis in SMMC-7721 Liver cancer cells by promoting the expression of FADD,caspase-8 and caspase-3 through a mitochondria-independent pathway.CONCLUSION Scolopentide,an antihepatoma peptide purified from centipedes,may inspire new antihepatoma agents.Scolopentide activates DR4 and DR5 and induces apoptosis in liver cancer cells through a mitochondria-independent pathway.
基金supported by grants from theNational Natural Science Foundation of China (No.81172345 and No. 30973506)the National High Technology Research and Development Program of China (863 Program) (2006AA02A404 to Y-X.Z.)
文摘Nucleophosmin/B23 (NPM) is a universally expressed nucleolar phosphoprotein that participates in proliferation, apoptosis, ribosome assembly, and centrosome duplication; however, the role of NPM in cell cycle regulation is not well characterized. We investigated the mechanism by which NPM is involved in cell cycle regulation. NPM was knocked down using siRNA in HepG2 hepatoblastoma cells. NPM translocation following actinomycin D (ActD) treatment was investigated using immunofluorescent staining. Expression of NPM and other factors involved in cell cycle regulation was examined by Western blotting. Cell cycle distribution was measured using flow cytometry to detect 5-ethynyl-2′-deoxyuridine (EdU) incorporation. Cell proliferation was quantified by the MTT assay. Knockdown of NPM increased the percentage of HepG2 cells in S phase and led to decreased expression of P53 and P21Cip1/WAF1. S-phase arrest in HepG2 cells was significantly enhanced by ActD treatment. Furthermore, knockdown of NPM abrogated ActD-induced G2/M phase cell cycle arrest. Taken together, these data demonstrate that inhibition of NPM has a significant effect on the cell cycle.
基金National Natural Science Foundation of China(82305090)Science and Technology Commission of Shanghai Municipality(22YF1448900)Shanghai Municipal Health Commission(20234Y0168).
文摘Objective To analyze the differences in the correlation of tongue image indicators among patients with benign lung nodules and lung cancer.Methods From July 1;2020 to March 31;2022;clinical information of lung cancer patients and benign lung nodules patients was collected at the Oncology Department of Longhua Hos-pital Affiliated to Shanghai University of Traditional Chinese Medicine and the Physical Ex-amination Center of Shuguang Hospital Affiliated to Shanghai University of Traditional Chi-nese Medicine;respectively.We obtained tongue images from patients with benign lung nod-ules and lung cancer using the TFDA-1 digital tongue diagnosis instrument;and analyzed these images with the TDAS V2.0 software.The extracted indicators included color space pa-rameters in the Lab system for both the tongue body(TB)and tongue coating(TC)(TB/TC-L;TB/TC-a;and TB/TC-b);textural parameters[TB/TC-contrast(CON);TB/TC-angular second moment(ASM);TB/TC-entropy(ENT);and TB/TC-MEAN];as well as TC parameters(perAll and perPart).The bivariate correlation of TB and TC features was analyzed using Pearson’s or Spearman’s correlation analysis;and the overall correlation was analyzed using canonical correlation analysis(CCA).Results Samples from 307 patients with benign lung nodules and 276 lung cancer patients were included after excluding outliers and extreme values.Simple correlation analysis indi-cated that the correlation of TB-L with TC-L;TB-b with TC-b;and TB-b with perAll in lung cancer group was higher than that in benign nodules group.Moreover;the correlation of TB-a with TC-a;TB-a with perAll;and the texture parameters of the TB(TB-CON;TB-ASM;TB-ENT;and TB-MEAN)with the texture parameters of the TC(TC-CON;TC-ASM;TC-ENT;and TC-MEAN)in benign nodules group was higher than lung cancer group.CCA further demon-strated a strong correlation between the TB and TC parameters in lung cancer group;with the first and second pairs of typical variables in benign nodules and lung cancer groups indicat-ing correlation coefficients of 0.918 and 0.817(P<0.05);and 0.940 and 0.822(P<0.05);re-spectively.Conclusion Benign lung nodules and lung cancer patients exhibited differences in correla-tion in the L;a;and b values of the TB and TC;as well as the perAll value of the TC;and the texture parameters(TB/TC-CON;TB/TC-ASM;TB/TC-ENT;and TB/TC-MEAN)between the TB and TC.Additionally;there were differences in the overall correlation of the TB and TC be-tween the two groups.Objective tongue diagnosis indicators can effectively assist in the diag-nosis of benign lung nodules and lung cancer;thereby providing a scientific basis for the ear-ly detection;diagnosis;and treatment of lung cancer.
基金National Natural Science Foundation of China(82104738 and 82004255)Key Discipline Construction Project of High-level Traditional Chinese Medicine of the National Administration of Traditional Chinese Medicine-Traditional Chinese Medical Diagnostics(ZYYZDXK-2023069).
文摘Objective To investigate the facial spectrum and color characteristics of patients with essen-tial hypertension post administering antihypertensive drugs,establish a classification and evaluation model based on the facial colors of the enrolled patients,and perform in-depth analysis on the important characteristics of their facial spectrum.Methods From September 3,2018,to March 23,2024,participants with essential hyperten-sion(receiving antihypertensive medication treatment,hypertension group)and normal blood pressure(control group)were recruited from the Cardiology Department of Shanghai Hospital of Traditional Chinese Medicine,the Coronary Care Unit of Shanghai Tenth People's Hospital,the Physical Examination Center of Shuguang Hospital Affiliated to Shanghai Uni-versity of Traditional Chinese Medicine,and the Gaohang Community Health Service Center.This study employed the propensity score matching(PSM)method to reduce study partici-pants selection bias.Spectral information in the facial visible light spectrum of the subjects was collected using a flame spectrometer,and the spectral chromaticity values were calculat-ed using the equal-interval wavelength method.The study analyzed the differences in spec-tral reflectance across various facial regions,including the entire face,forehead,glabella,nose,jaw,left and right zygomatic regions,left and right cheek regions as well as differences in parameters within the Lab color space between the two subject groups.Feature selection was conducted using least absolute shrinkage and selection operator(LASSO)regression,fol-lowed by the application of various machine learning algorithms,including logistic regres-sion(LR),support vector machine(SVM),random forest(RF),Naïve Bayes(NB),and eX-treme Gradient Boosting(XGB).The reduced-dimensional dataset was split in a 7:3 ratio to establish a classification and assessment model for facial coloration related to primary hyper-tension.Additionally,model fusion techniques were applied to enhance the predictive power.The performance of the models was evaluated using metrics including the area under the curve(AUC)and accuracy.Shapley Additive exPlanations(SHAP)was used to interpret the outcomes of the models.Results A total of 114 participants were included in both hypertension and control groups.Reflectance analysis across the entire face and eight predefined areas revealed that the hypertensive group exhibited significantly higher reflectance of corresponding color light in the blue-violet region(P<0.05)and a lower reflectance in the red region(P<0.05)compared with control group.Analysis of Lab color space parameters across the entire face and eight predefined areas showed that hypertensive group had significantly lower a and b values than control group(P<0.05).LASSO regression analysis identified a total of 18 facial color features that were highly correlated with hypertension,including the a values of the chin and the right cheek,the reflectance at 380 nm and at 780 nm of the forehead.The results of the multi-mod-el classification showed that the RF classification model was the most effective,with an AUC of 0.74 and an accuracy of 0.77.The combined model of RF+LR+SVM outperformed a single model in their classification performance,achieving an AUC of 0.80 and an accuracy of 0.76.SHAP model visualization results indicated that the top three contributors to ideal prediction results based on the characteristics from the facial spectrum were the reflectance at 380 nm across the entire face and of the nose as well as the a value of the chin.Conclusion Within the same age group,patients with essential hypertension exhibited signif-icant and regular changes in facial color and facial spectral reflectance parameters after the administration of antihypertensive drugs.Furthermore,facial reflectance indicators,such as the overall reflectance at 380 nm and the a value of the chin,could offer valuable references for clinically assessing the drug efficacy and health status of patients with essential hyperten-sion.
文摘Last year,the doubling of the A-share market was unmatched in global capital markets.Fu- eled by appreciation of the RMB,sectors like finance and real estate as well newly revitalized areas like non- ferrous metals,the steel and chemical industries,drove market growth,creat- ing many millionaires in the process. In terms of pure performance,all of the 13 sectors classified by the CSRC had strong performance with growth
文摘The National Development and Reform Commission of China (NDRC) released on August 27 a report on the first-half operation and the second-half expectation of the coal industry.The coal industry is predicted to have a consecutive balance of sup- ply and demand this year with a less strained supply in the second half.
文摘According to customs statistics, Chinese trade imports and exports totaled US$ 1,759.32 billion from January to October this year,representing an increase of 23.5 percent over the same period last year, and nearly equal to the total amount of imports and exports from all of last year.Exports were US$ 985.84
文摘The'three most-wanted'goods,those seen as a symbol of wealth for Chinese families, has seen an evolution from specific products to general services with average values that first increased from RMB 100 to RMB 10,000,and now have moved upwards of RMB 100,000.While this measure is not the most scientific,it reflects the great changes China has undergone,and also mirrors the transformation of Chinese lifestyle.
文摘Aseries of events that occurred in October 2007,are rewrit- ing the history of China's en- ergy industry:November 3 an Energy Law Committee asked the public for suggestions on improvements;forty top experts came together to form the National Energy Expert Advisory Committee on November 6;and the National Oil Reserve Center(NORC)
文摘The Office of the National Ener- gy Leading Group announced a draft of the upcoming energy law on December 3,2007,in an effort to collect feedback from the public and the media through the Internet up to the 1 of February 2008.This marks the first time that the draft of a new energy law has been released to the public since research and study work began in 2006;the event also symbolizes a new phase for energy legislation.
