Objective:To investigate hemodynamic change of patients with cirrhosis by using Color Doppler ultrasound technique and to explore the significance of change in the content of vasoactive substances-plasma endothelin-1(...Objective:To investigate hemodynamic change of patients with cirrhosis by using Color Doppler ultrasound technique and to explore the significance of change in the content of vasoactive substances-plasma endothelin-1(ET-1)and calcitonin gene-related peptide(CGRP).Methods:A total of 178 cases with cirrhosis were regarded as study groups,and were divided into three degrees:A,B and C according to child-pugh and meanwhile 60 cases were regarded as normal control group.Portal vein and splenic vein of patients were explored by adopting Color Doppler ultrasound technique,related indexes were recorded and the blood flow as well as their ration in the two groups was calculated.Radio immunoassay was adopted to detect the content of plasma ET-1 and CGRP in both study group and contrast group.Results:Compared with the healthy cases in the contrast group,there was abnormal hemodynamics in the system of portal vein of patients with cirrhosis and the content of plasma ET-1 and CGRP was increased obviously.In the Child-Pugh liver function grades,the content of ET-1 and CGRP was increased as the degree of cirrhosis became more and more serious.There was no significant difference in the comparison between those without ascites and those in contrast group(P>0.05),the content of plasma ET-1and CGRP in patients without ascites was increased remarkably.Besides,there was positive correlation between the content of plasma ET-1 and CGRP and Dpv,Dsv and Qsv.Conclusion:Dotection of abnormal hemodynamics of portal vein and splenic vein by Color Doppler ultrasound technique can be one of the means for diagnosis of hypertension.Plasma ET-1 and CGRP of patients with cirrhosis reflect the serious degree of the damage in live function and play an important role in the formation and development of portal hypertension.展开更多
Objective:To investigate the role of epidermal growth factor(EGF) in the pathogenesis of uterine leiomyomas.Methods:Human myometrial smooth muscle cells(HM-SMCs) and smooth muscle cells of human uterine leiomyomas(HL-...Objective:To investigate the role of epidermal growth factor(EGF) in the pathogenesis of uterine leiomyomas.Methods:Human myometrial smooth muscle cells(HM-SMCs) and smooth muscle cells of human uterine leiomyomas(HL-SMCs) were separated from patients' specimens and cultured.After processed by EGF or PD98059(inhibitor of MKK/MEK) +EGF,the proliferation rate of both SMCs was detected by BrdU method and the phosphorylation level of p44/42 mitogen-activated protein kinase(MAPK) was determined by Western-blot.After different processing time by EGF,the phosphorylation levels of p44/42 MAPK and AKT and p27 expression level in both SMCs were detected by Western-blot.Results:EGF could significantly promote HL-SMCs proliferation and PD98059 could inhibit this effect(P<0.05);besides,PD98059 could inhibit the increase of the phosphorylation level of p44/42 MAPK in both SMCs induced by EGF.When the processing time by EGF was over 15 min,the phosphorylation levels of p44/42 MAPK and AKT in both SMCs decreased sharply and were close to zero:p27 expression in HM-SMCs raised significantly while the upregulation in HL-SMCs was little.Conclusions:EGF could not cause activation of EGFR because of the dephosphorylation of p44/42 MAPK and AKT in HL-SMCs,which caused p27 expression insufficiently and cell cycle dysregulation.展开更多
Objective:To discuss the liver function damage mechanism of patients with clonorchiasis by analyzing the ultrasound characteristics,liver function,change of the serum inflammatory factors and cell apoptosis factors.Me...Objective:To discuss the liver function damage mechanism of patients with clonorchiasis by analyzing the ultrasound characteristics,liver function,change of the serum inflammatory factors and cell apoptosis factors.Methods:Color Doppler ultrasound technique was adopted to detect the portal vein and blood flow change of patients with clonorchiasis;ELISA was used to determine the level of different serum inflammatory factors.The levels of serum total bilirubin,serum albumin and glutamic-pyruvic transaminase were detected by automatic biochemical analyzer.Western blot was used to determine the expression of proteins relevant to apoptosis.Results:Compared with the health control group,the trunk diameter of portal vein and the thickness of spleen,as well as the hepatic artery pulsation index of clonorchiasis patients increased obviously,the mean blood flow velocity of portal vein(P<0.05 or P<0.01) decreased.The content of total bilirubin and transaminase in plasma increased significantly,but albumin decreased(P<0.05).Levels of TNF-α,IL-6 and IFN-γ increased remarkably,and the level of every factor was significantly different among patients with Child-Pugh Ⅰ,Child-Pugh Ⅱ and Child-Pugh Ⅲ classification of liver function(P<0.05 or P<0.01).With the exacerbation of liver dysfunction,levels of TNF-α,IL-6 and IFN-γ gradually increased(P<0.05).Compared with the healthy control group,the expression quantity of apoptosis protein Fas,FasL,Bax and Caspase-3 increased significantly(P<0.05 or P<0.01),but Bcl-2 decreased(P<0.05).Conclusions:Changes of ultrasonic characteristics and liver dysfunction,caused by liver fluke infection,may be related to that both inflammatory response and apoptosis response have participated in the pathogenic process and liver damage course of clonorchiasis.展开更多
Binary digit representation of partial sums for random variables has been investigated, and a good upper bound of moments of maximum partial sums for random variables has been reduced by using this representation. As ...Binary digit representation of partial sums for random variables has been investigated, and a good upper bound of moments of maximum partial sums for random variables has been reduced by using this representation. As an applications, stability and strong law of large numbers have been discussed. Many known classical results have been refined.展开更多
Streptomyces sp.MJM3502 is a promising producer of rufomycins,which are a class of potent anti-tuberculosis lead compounds.Although the structure,activity,and mechanism of the main rufomycin 4/6 and its analogs have b...Streptomyces sp.MJM3502 is a promising producer of rufomycins,which are a class of potent anti-tuberculosis lead compounds.Although the structure,activity,and mechanism of the main rufomycin 4/6 and its analogs have been extensively studied,a significant gap remains in our understanding of the genome sequence and biosynthetic pathway of Streptomyces sp.MJM3502,and its metabolic engineering has not yet been reported.This study established the genetic manipulation platform for the strain.Using CRISPR/Cas9-based technology to in-frame insert the strong kasO^(*)p promoter upstream of the rufB and rufS genes of the rufomycin BGC,we increased rufomycin 4/6 production by 4.1-fold and 2.8-fold,respectively.Furthermore,designing recombinant strains by inserting the kasO^(*)p promoter upstream of the biosynthetic genes encoding cytochrome P450 enzymes led to new rufomycin derivatives.These findings provide the basis for enhancing the production of valuable natural compounds in Streptomyces and offer insights into the generation of novel active natural products via synthetic biology and metabolic engineering.展开更多
Some results in the law of logarithm for NA sequences are obtained, which are similar to the result of id random variables; as a corollary, a sufficient result for the bounbed law of logarithm for NA arrays is given. ...Some results in the law of logarithm for NA sequences are obtained, which are similar to the result of id random variables; as a corollary, a sufficient result for the bounbed law of logarithm for NA arrays is given. Meanwhile, A conjecture for id random variables of Gut is answered in NA setting. Under weaker moment conditions than Su and Qin’s, a stronger convergence resuit than their Theorem 1 is obtained.展开更多
Let {X（t）, t ≥ 0} be a Lévy process with EX（1） = 0 and EX^2（1） 〈 ∞. In this paper, we shall give two precise asymptotic theorems for {X（t）, t 〉 0}. By the way, we prove the corresponding conclusions f...Let {X（t）, t ≥ 0} be a Lévy process with EX（1） = 0 and EX^2（1） 〈 ∞. In this paper, we shall give two precise asymptotic theorems for {X（t）, t 〉 0}. By the way, we prove the corresponding conclusions for strictly stable processes and a general precise asymptotic proposition for sums of i.i.d. random variables.展开更多
基金supported by a grant of New Century Excellent Talents in University,Ministry of Education China(No.NCET-11-0949)
文摘Objective:To investigate hemodynamic change of patients with cirrhosis by using Color Doppler ultrasound technique and to explore the significance of change in the content of vasoactive substances-plasma endothelin-1(ET-1)and calcitonin gene-related peptide(CGRP).Methods:A total of 178 cases with cirrhosis were regarded as study groups,and were divided into three degrees:A,B and C according to child-pugh and meanwhile 60 cases were regarded as normal control group.Portal vein and splenic vein of patients were explored by adopting Color Doppler ultrasound technique,related indexes were recorded and the blood flow as well as their ration in the two groups was calculated.Radio immunoassay was adopted to detect the content of plasma ET-1 and CGRP in both study group and contrast group.Results:Compared with the healthy cases in the contrast group,there was abnormal hemodynamics in the system of portal vein of patients with cirrhosis and the content of plasma ET-1 and CGRP was increased obviously.In the Child-Pugh liver function grades,the content of ET-1 and CGRP was increased as the degree of cirrhosis became more and more serious.There was no significant difference in the comparison between those without ascites and those in contrast group(P>0.05),the content of plasma ET-1and CGRP in patients without ascites was increased remarkably.Besides,there was positive correlation between the content of plasma ET-1 and CGRP and Dpv,Dsv and Qsv.Conclusion:Dotection of abnormal hemodynamics of portal vein and splenic vein by Color Doppler ultrasound technique can be one of the means for diagnosis of hypertension.Plasma ET-1 and CGRP of patients with cirrhosis reflect the serious degree of the damage in live function and play an important role in the formation and development of portal hypertension.
基金supported by Program for New Century Excellent Talents in University(NCET-11-0949)
文摘Objective:To investigate the role of epidermal growth factor(EGF) in the pathogenesis of uterine leiomyomas.Methods:Human myometrial smooth muscle cells(HM-SMCs) and smooth muscle cells of human uterine leiomyomas(HL-SMCs) were separated from patients' specimens and cultured.After processed by EGF or PD98059(inhibitor of MKK/MEK) +EGF,the proliferation rate of both SMCs was detected by BrdU method and the phosphorylation level of p44/42 mitogen-activated protein kinase(MAPK) was determined by Western-blot.After different processing time by EGF,the phosphorylation levels of p44/42 MAPK and AKT and p27 expression level in both SMCs were detected by Western-blot.Results:EGF could significantly promote HL-SMCs proliferation and PD98059 could inhibit this effect(P<0.05);besides,PD98059 could inhibit the increase of the phosphorylation level of p44/42 MAPK in both SMCs induced by EGF.When the processing time by EGF was over 15 min,the phosphorylation levels of p44/42 MAPK and AKT in both SMCs decreased sharply and were close to zero:p27 expression in HM-SMCs raised significantly while the upregulation in HL-SMCs was little.Conclusions:EGF could not cause activation of EGFR because of the dephosphorylation of p44/42 MAPK and AKT in HL-SMCs,which caused p27 expression insufficiently and cell cycle dysregulation.
基金supported by Trans-Century Training Programme Foundation for the Talents by the Ministry of Education (NO.NCET-11-0949)
文摘Objective:To discuss the liver function damage mechanism of patients with clonorchiasis by analyzing the ultrasound characteristics,liver function,change of the serum inflammatory factors and cell apoptosis factors.Methods:Color Doppler ultrasound technique was adopted to detect the portal vein and blood flow change of patients with clonorchiasis;ELISA was used to determine the level of different serum inflammatory factors.The levels of serum total bilirubin,serum albumin and glutamic-pyruvic transaminase were detected by automatic biochemical analyzer.Western blot was used to determine the expression of proteins relevant to apoptosis.Results:Compared with the health control group,the trunk diameter of portal vein and the thickness of spleen,as well as the hepatic artery pulsation index of clonorchiasis patients increased obviously,the mean blood flow velocity of portal vein(P<0.05 or P<0.01) decreased.The content of total bilirubin and transaminase in plasma increased significantly,but albumin decreased(P<0.05).Levels of TNF-α,IL-6 and IFN-γ increased remarkably,and the level of every factor was significantly different among patients with Child-Pugh Ⅰ,Child-Pugh Ⅱ and Child-Pugh Ⅲ classification of liver function(P<0.05 or P<0.01).With the exacerbation of liver dysfunction,levels of TNF-α,IL-6 and IFN-γ gradually increased(P<0.05).Compared with the healthy control group,the expression quantity of apoptosis protein Fas,FasL,Bax and Caspase-3 increased significantly(P<0.05 or P<0.01),but Bcl-2 decreased(P<0.05).Conclusions:Changes of ultrasonic characteristics and liver dysfunction,caused by liver fluke infection,may be related to that both inflammatory response and apoptosis response have participated in the pathogenic process and liver damage course of clonorchiasis.
文摘Binary digit representation of partial sums for random variables has been investigated, and a good upper bound of moments of maximum partial sums for random variables has been reduced by using this representation. As an applications, stability and strong law of large numbers have been discussed. Many known classical results have been refined.
基金supported by grants from“Cooperative Research Program for Agriculture Science and Technology Development(Project No.PJ01319101)”Rural Development Administration,Republic of KoreaThe National Natural Science Foundation of China(Project No.31600038)+1 种基金the Key Research Development Program of the Natural Science Basic Research Plan in Shaanxi Province of China(Project No.2021NY-196)funding of their anti-TB cyclopetide research by grant U19AT142735.
文摘Streptomyces sp.MJM3502 is a promising producer of rufomycins,which are a class of potent anti-tuberculosis lead compounds.Although the structure,activity,and mechanism of the main rufomycin 4/6 and its analogs have been extensively studied,a significant gap remains in our understanding of the genome sequence and biosynthetic pathway of Streptomyces sp.MJM3502,and its metabolic engineering has not yet been reported.This study established the genetic manipulation platform for the strain.Using CRISPR/Cas9-based technology to in-frame insert the strong kasO^(*)p promoter upstream of the rufB and rufS genes of the rufomycin BGC,we increased rufomycin 4/6 production by 4.1-fold and 2.8-fold,respectively.Furthermore,designing recombinant strains by inserting the kasO^(*)p promoter upstream of the biosynthetic genes encoding cytochrome P450 enzymes led to new rufomycin derivatives.These findings provide the basis for enhancing the production of valuable natural compounds in Streptomyces and offer insights into the generation of novel active natural products via synthetic biology and metabolic engineering.
文摘Some results in the law of logarithm for NA sequences are obtained, which are similar to the result of id random variables; as a corollary, a sufficient result for the bounbed law of logarithm for NA arrays is given. Meanwhile, A conjecture for id random variables of Gut is answered in NA setting. Under weaker moment conditions than Su and Qin’s, a stronger convergence resuit than their Theorem 1 is obtained.
基金supported by the National Natural Science Foundation(Grant No.10671188)Special Foundation of USTC
文摘Let {X（t）, t ≥ 0} be a Lévy process with EX（1） = 0 and EX^2（1） 〈 ∞. In this paper, we shall give two precise asymptotic theorems for {X（t）, t 〉 0}. By the way, we prove the corresponding conclusions for strictly stable processes and a general precise asymptotic proposition for sums of i.i.d. random variables.
