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基于内皮细胞/周细胞构建大鼠视网膜血管新生体外三维模型 被引量:3
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作者 刘光辉 杨田野 +4 位作者 洪雅军 王航 潘铭东 孟春 徐朝阳 《国际眼科杂志》 CAS 北大核心 2021年第9期1520-1523,共4页
目的:构建基于视网膜微血管内皮细胞(ECs)和周细胞(RMPs)的大鼠视网膜血管新生体外三维模型。方法:分离、纯化、培养大鼠视网膜微血管内皮细胞及视网膜微血管周细胞,选用第3~7代经鉴定后的细胞用于研究,采用细胞示踪剂对细胞进行染色,... 目的:构建基于视网膜微血管内皮细胞(ECs)和周细胞(RMPs)的大鼠视网膜血管新生体外三维模型。方法:分离、纯化、培养大鼠视网膜微血管内皮细胞及视网膜微血管周细胞,选用第3~7代经鉴定后的细胞用于研究,采用细胞示踪剂对细胞进行染色,混合后按表面培养法接种于Matrigel,动态观察,并检测血管新生期间血管内皮生长因子A(VEGF-A)的表达。结果:培养12h时,RMPs被ECs招募,聚集成大小不等细胞团块;24h时,ECs/RMPs共同形成复杂的三维血管样条索网;48h时,网状结构出现明显崩解,仅保持少量不完整的简单网状结构;72h时,血管样条索网完全崩解。在三维模型发生过程中,VEGF-A的表达量升高;退化时,VEGF-A的表达量下降。结论:成功基于视网膜微血管全成分细胞ECs和RMPs构建了大鼠视网膜血管新生体外三维模型。 展开更多
关键词 视网膜微血管 内皮细胞 周细胞 血管新生 三维模型
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Interaction Between Memory Load and Experimental Design on Brain Connectivity and Network Topology 被引量:1
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作者 Heming Zhang Xin Di +3 位作者 Bart Rypma Hang Yang chun meng Bharat Biswal 《Neuroscience Bulletin》 SCIE CAS CSCD 2023年第4期631-644,共14页
The conventional approach to investigating functional connectivity in the block-designed study usually concatenates task blocks or employs residuals of task activation.While providing many insights into brain function... The conventional approach to investigating functional connectivity in the block-designed study usually concatenates task blocks or employs residuals of task activation.While providing many insights into brain functions,the block design adds more manipulation in functional network analysis that may reduce the purity of the blood oxygenation level-dependent signal.Recent studies utilized one single long run for task trials of the same condition,the so-called continuous design,to investigate functional connectivity based on task functional magnetic resonance imaging.Continuous brain activities associated with the single-task condition can be directly utilized for task-related functional connectivity assessment,which has been examined for working memory,sensory,motor,and semantic task experiments in previous research.But it remains unclear how the block and continuous design influence the assessment of task-related functional connectivity networks.This study aimed to disentangle the separable effects of block/continuous design and working memory load on task-related functional connectivity networks,by using repeated-measures analysis of variance.Across 50 young healthy adults,behavioral results of accuracy and reaction time showed a significant main effect of design as well as interaction between design and load.Imaging results revealed that the cingulo-opercular,fronto-parietal,and default model networks were associated with not only task activation,but significant main effects of design and load as well as their interaction on intra-and inter-network functional connectivity and global network topology.Moreover,a significant behavior-brain association was identified for the continuous design.This work has extended the evidence that continuous design can be used to study task-related functional connectivity and subtle brain-behavioral relationships. 展开更多
关键词 Working memory Functional connectivity Network topology Block design Continuous design Memory load
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An Overview of Privacy Preserving Schemes for Industrial Internet of Things 被引量:1
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作者 Yan Huo chun meng +1 位作者 Ruinian Li Tao Jing 《China Communications》 SCIE CSCD 2020年第10期1-18,共18页
The concept of Internet of Everything is like a revolutionary storm,bringing the whole society closer together.Internet of Things(IoT)has played a vital role in the process.With the rise of the concept of Industry 4.0... The concept of Internet of Everything is like a revolutionary storm,bringing the whole society closer together.Internet of Things(IoT)has played a vital role in the process.With the rise of the concept of Industry 4.0,intelligent transformation is taking place in the industrial field.As a new concept,an industrial IoT system has also attracted the attention of industry and academia.In an actual industrial scenario,a large number of devices will generate numerous industrial datasets.The computing efficiency of an industrial IoT system is greatly improved with the help of using either cloud computing or edge computing.However,privacy issues may seriously harmed interests of users.In this article,we summarize privacy issues in a cloud-or an edge-based industrial IoT system.The privacy analysis includes data privacy,location privacy,query and identity privacy.In addition,we also review privacy solutions when applying software defined network and blockchain under the above two systems.Next,we analyze the computational complexity and privacy protection performance of these solutions.Finally,we discuss open issues to facilitate further studies. 展开更多
关键词 privacy preserving cloud computing edge computing industrial Internet of Things
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A Study on the Relationship Between Land Finance and Housing Price in Urbanization Process: An Empirical Analysis of 182 Cities in China Based on Threshold Panel Models 被引量:3
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作者 Meiting HU Jichang DONG +2 位作者 Lijun YIN chun meng Xiuting LI 《Journal of Systems Science and Information》 CSCD 2021年第1期74-94,共21页
Scholars have a variety of theoretical explanations for housing price growth. However, few scholars have studied the internal influence mechanism among urbanization, land finance, and housing price. Based on the data ... Scholars have a variety of theoretical explanations for housing price growth. However, few scholars have studied the internal influence mechanism among urbanization, land finance, and housing price. Based on the data of 182 prefecture-level cities from 2009 to 2016, this paper studies the influence of land finance on housing price under different urbanization rate levels. The study finds that with the increase of urbanization rate, the effect of land finance on housing price presents a "U" shape.Specifically, an increase in land finance by 1% results in a corresponding increase in average housing price by 0.18%, with relatively low urbanization rate, 0.06% with medium level of urbanization rate,and 0.38% with high level of urbanization rate. 展开更多
关键词 URBANIZATION land finance housing price threshold panel model
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Development of a Rapid and Convenient Method for Sampling Airborne Virus based on Nanopar-ticle Adsorption 被引量:1
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作者 chun meng Yulin Xiong +3 位作者 Weiliang Zhuang Hang Wang Xian'ai Shi Yanghao Guo 《Nano Biomedicine & Engineering》 2010年第3期171-176,共6页
An improved aerobiological virus sampling method was developed based on adding adsorptive nanoparticles in samplers for concentrating viruses in sampling liquid buffers.The objectives of this research were to select e... An improved aerobiological virus sampling method was developed based on adding adsorptive nanoparticles in samplers for concentrating viruses in sampling liquid buffers.The objectives of this research were to select effective adsorptive materials and optimize sampling parameters for increasing recovery of airborne viruses,such as influenza A virus or respiratory syndrome virus(RSV).Three kinds of polycation nanoparticles were evaluated for direct effects on absorption and desorption of influenza virus hemagglutinin and DNA.Chitosan particles showed good performance in absorption and desorption for both influenza virus hemagglutinin and DNA.A subsequent study evaluated the effects of collection buffer,pH and sampling time on the recovery of aerosolized viruses using a method for making direct comparisons of three treatments.The results demonstrated that various components in air-sampling collection buffer,impinger model,and sampling time,independently influenced the recovery of viruses.It was shown that adsorptive samplers with air disperser had the highest levels of sensitivity and repeatability in virus sampling.Both unspecifically adsorptive chitosan particles and specifically adsorptive particles labeled specific antibody to virus significantly enhanced recovery rate of aerosolized viruses.We succeeded to sample low level different pathogen viruses in outdoor environments with the optimized sampling system. 展开更多
关键词 Aerobiological virus sampling method ADSORPTION Polycation chitosan nanoparticles Influenza virus Air disperser
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