Background:Makorin ring finger protein 3 gene(MKRN3)gene mutation is the most common genetic cause of central precocious puberty(CPP)in children.Due to the lack of ideal MKRN3-modified animal model(MKRN3-modified mice...Background:Makorin ring finger protein 3 gene(MKRN3)gene mutation is the most common genetic cause of central precocious puberty(CPP)in children.Due to the lack of ideal MKRN3-modified animal model(MKRN3-modified mice enter puberty only 4–5 days earlier than normal mice),the related research is limited.Methods:Therefore,the MKRN3-modified rabbit was developed using CRISPR(clus-tered regularly interspaced short palindromic repeats)gene editing technology.The genotype identification and phenotype evaluation of MKRN3-modified rabbits were carried out.Results:The first estrus of MKRN3-modified female rabbits was observed~27 days earlier than that of wild-type female rabbits,with a typical CPP phenotype.This study found increased gonadotropin releasing hormone(GnRH)and decreased gonadotropin inhibiting hormone(GnIH)in the hypothalamus of the CPP rabbit model with MKRN3 gene mutation.Although this study failed to fully clarify the pathogenesis of CPP caused by MKRN3 mutation,it found some differentially expressed genes and potential pathways through transcriptome sequencing.Conclusions:This study established a novel CPP model:paternal MKRN3 gene-modified rabbit.It is hoped that the establishment of this model will help researchers better understand,treat,and prevent CPP in the future.展开更多
Soil moisture(SM)plays a crucial role in the dynamics of coupled atmosphere,water,energy,and carbon cycles.In this study,the spatiotemporal distribution and variations of SM in the Mongolian Plateau were investigated ...Soil moisture(SM)plays a crucial role in the dynamics of coupled atmosphere,water,energy,and carbon cycles.In this study,the spatiotemporal distribution and variations of SM in the Mongolian Plateau were investigated for the period of 1982-2018 based on four gridded SM datasets.Taking the observed SM from 33 monitoring stations as a reference,the reliability of these four gridded products was validated,and the three-cornered hat(TCH)method was also applied to evaluate their uncertainties.Major results indicated that all these four products underestimated the SM in this region at different levels.The ERA-Interim SM performed much better than other three products,with a higher R of 0.53 and lower ubRMSE of 0.0378 m^(3)·m^(−3).Except for ECV,the surface SM exhibited a decreasing trend at−0.0004 to−0.0008 m^(3)·m^(−3)/decade in the warm season(from April to October)over the past 37 years.The greatest decreases in SM occurred in the summer season.Precipitation change plays a more important role in explaining the variations of SM than temperature reported by partial correlation analysis.The results of this study will benefit our understanding of the climate change effects and the protection of the ecological systems in this region.展开更多
Traditional Mongolian medicine(TMM)is an important part of Chinese traditional culture,which plays an important role within the medical system of China.The processing of Mongolian medicinal materials is a pharmaceutic...Traditional Mongolian medicine(TMM)is an important part of Chinese traditional culture,which plays an important role within the medical system of China.The processing of Mongolian medicinal materials is a pharmaceutical technology,which is the unique characteristics of Mongolian medicine.In this paper,the basic concepts related to the processing of Mongolian medicinal materials were introduced,and its scientific research points were put forward,in order to deeply excavate the connotation of Mongolian pharmacy and further study the processing mechanism of Mongolian medicinal materials,so as to provide important basis for the development of Chinese traditional medicine.The essence of Mongolian medicinal materials processing is to use drugs safely and dialectically to ensure the quality of Mongolian medicinal materials.The scientific research sites of Mongolian medicinal materials processing have two categories:reducing toxicity(increasing)effect and synergistic effect of excipients and processing factors.Because of the not perfect research platform of Mongolian medicinal materials and the weak processing power,the development of research of Mongolian medicinal materials is relatively slow.Therefore,there are many research breakthroughs in the interdisciplinary research on the processing of Mongolian medicinal materials,and it is expected to become a research hotspot.展开更多
基金National Natural Science Foundation of China,Grant/Award Number:82101937Guangdong Medical Science and Technology Research Fund Project,China,Grant/Award Number:B2024069Guangzhou Science and Technology Plan Project,China,Grant/Award Number:2024A04J4923 and SL2023A04J02229。
文摘Background:Makorin ring finger protein 3 gene(MKRN3)gene mutation is the most common genetic cause of central precocious puberty(CPP)in children.Due to the lack of ideal MKRN3-modified animal model(MKRN3-modified mice enter puberty only 4–5 days earlier than normal mice),the related research is limited.Methods:Therefore,the MKRN3-modified rabbit was developed using CRISPR(clus-tered regularly interspaced short palindromic repeats)gene editing technology.The genotype identification and phenotype evaluation of MKRN3-modified rabbits were carried out.Results:The first estrus of MKRN3-modified female rabbits was observed~27 days earlier than that of wild-type female rabbits,with a typical CPP phenotype.This study found increased gonadotropin releasing hormone(GnRH)and decreased gonadotropin inhibiting hormone(GnIH)in the hypothalamus of the CPP rabbit model with MKRN3 gene mutation.Although this study failed to fully clarify the pathogenesis of CPP caused by MKRN3 mutation,it found some differentially expressed genes and potential pathways through transcriptome sequencing.Conclusions:This study established a novel CPP model:paternal MKRN3 gene-modified rabbit.It is hoped that the establishment of this model will help researchers better understand,treat,and prevent CPP in the future.
基金supported by Natural Science Foundation of Inner Mongolia:[Grant Number 2020BS03042,2020BS04009]the National Natural Science Foundation of China:[Grant Number 41861014]+1 种基金the Open Project of Remote Sensing and Geographic Information System Application Laboratory,State Key Laboratory of Xinjiang Uygur Autonomous Region:[Grant Number 112/21K20190016]the Research Start-up Fund Project for Introducing Talents:[Grant Number 112/1004031904,112/5909001803].
文摘Soil moisture(SM)plays a crucial role in the dynamics of coupled atmosphere,water,energy,and carbon cycles.In this study,the spatiotemporal distribution and variations of SM in the Mongolian Plateau were investigated for the period of 1982-2018 based on four gridded SM datasets.Taking the observed SM from 33 monitoring stations as a reference,the reliability of these four gridded products was validated,and the three-cornered hat(TCH)method was also applied to evaluate their uncertainties.Major results indicated that all these four products underestimated the SM in this region at different levels.The ERA-Interim SM performed much better than other three products,with a higher R of 0.53 and lower ubRMSE of 0.0378 m^(3)·m^(−3).Except for ECV,the surface SM exhibited a decreasing trend at−0.0004 to−0.0008 m^(3)·m^(−3)/decade in the warm season(from April to October)over the past 37 years.The greatest decreases in SM occurred in the summer season.Precipitation change plays a more important role in explaining the variations of SM than temperature reported by partial correlation analysis.The results of this study will benefit our understanding of the climate change effects and the protection of the ecological systems in this region.
基金supported by National Natural Science Foundation of China (No.81560705)Inner Mongolia Natural Science Foundation of China (No.2020LH0800)
文摘Traditional Mongolian medicine(TMM)is an important part of Chinese traditional culture,which plays an important role within the medical system of China.The processing of Mongolian medicinal materials is a pharmaceutical technology,which is the unique characteristics of Mongolian medicine.In this paper,the basic concepts related to the processing of Mongolian medicinal materials were introduced,and its scientific research points were put forward,in order to deeply excavate the connotation of Mongolian pharmacy and further study the processing mechanism of Mongolian medicinal materials,so as to provide important basis for the development of Chinese traditional medicine.The essence of Mongolian medicinal materials processing is to use drugs safely and dialectically to ensure the quality of Mongolian medicinal materials.The scientific research sites of Mongolian medicinal materials processing have two categories:reducing toxicity(increasing)effect and synergistic effect of excipients and processing factors.Because of the not perfect research platform of Mongolian medicinal materials and the weak processing power,the development of research of Mongolian medicinal materials is relatively slow.Therefore,there are many research breakthroughs in the interdisciplinary research on the processing of Mongolian medicinal materials,and it is expected to become a research hotspot.
