Objective and Impact Statement:We describe an electroenzymatic mediator(EM)sensor based on an electroenzymatic assembly peak separation strategy,which can efficiently realize the simultaneous detection of 3 typical ca...Objective and Impact Statement:We describe an electroenzymatic mediator(EM)sensor based on an electroenzymatic assembly peak separation strategy,which can efficiently realize the simultaneous detection of 3 typical cardiovascular disease(CVD)metabolites in 5μl of plasma under one test.This work has substantial implications toward improving the efficiency of chronic CVD assessment.Introduction:Monitoring CVD of metabolites is strongly associated with disease risk.Independent and time-consuming detection in hospitals is unfavorable for chronic CVD management.Methods:The EM was flexibly designed by the cross-linking of electron mediators and enzymes,and 3 EM layers with different characteristics were assembled on one electrode.Electrons were transferred under tunable potential;3 metabolites were quantitatively detected by 3 peak currents that correlated with metabolite concentrations.Results:In this study,the EM sensor showed high sensitivity for the simultaneous detection of 3 metabolites with a lower limit of 0.01 mM.The linear correlation between the sensor and clinical was greater than 0.980 for 242 patients,and the consistency of risk assessment was 94.6%.Conclusion:Metabolites could be expanded by the EM,and the sensor could be a promising candidate as a home healthcare tool for CVD risk assessment.展开更多
Circulating tumor cells(CTCs)have tremendous potential to indicate disease progression and monitor therapeutic response using minimally invasive approaches.Considering the limitations of affinity strategies based on t...Circulating tumor cells(CTCs)have tremendous potential to indicate disease progression and monitor therapeutic response using minimally invasive approaches.Considering the limitations of affinity strategies based on their cost,effectiveness,and simplicity,size-based enrichment methods that involve low-cost,label-free,and relatively simple protocols have been further promoted.Nevertheless,the key challenges of these methods are clogging issues and cell aggregation,which reduce the recovery rates and purity.Inspired by the natural phenomenon that the airflow around a windmill is disturbed,in this study,a windmill-like hole array on the SU-8 membrane was designed to perturb the fluid such that cells in a fluid would be able to self-mix and that the pressure acting on cells or the membrane would be dispersed to allow a greater velocity.In addition,based on the advantages of fluid coatings,a lipid coating was used to modify the membrane surface to prevent cell aggregation and clogging of the holes.Under the optimal conditions,recovery rates of 93%and 90%were found for A549 and HeLa cells in a clinical simulation test of our platform with a CTC concentration of 20-100 cells per milliliter of blood.The white blood cell(WBC)depletion rate was 98.7%(n=15),and the CTC detection limit was less than 10 cells per milliliter of blood(n=6).Moreover,compared with conventional membrane filtration,the advantages of the proposed device for the rapid(2 mL/min)and efficient enrichment of CTCs without clogging were shown both experimentally and theoretically.Due to its advantages in the efficient,rapid,uniform,and clog-free enrichment of CTCs,our platform offers great potential for metastatic detection and therapy analyses.展开更多
Spatial monoomics has been recognized as a powerful tool for exploring life sciences.Recently,spatial multiomics has advanced considerably,which could contribute to clarifying many biological issues.Spatial monoomics ...Spatial monoomics has been recognized as a powerful tool for exploring life sciences.Recently,spatial multiomics has advanced considerably,which could contribute to clarifying many biological issues.Spatial monoomics techniques in epigenomics,genomics,transcriptomics,proteomics,and metabolomics can enhance our understanding of biological functions and cellular identities by simultaneously measuring tissue structures and biomolecule levels.Spatial monoomics technology has evolved from monoomics to spatial multiomics.Moreover,the spatial resolution,high-throughput detection capability,capture efficiency,and compatibility with various sample types of omics technology have considerably advanced.Despite the technological advances in this field,data analysis frameworks have stagnated.Current challenges include incomplete spatial monoomics data analysis pipeline,overly complex data analysis tasks,and few established spatial multiomics data analysis strategies.In this review,we systematically summarize recent developments of various spatial monoomics techniques and improvements in related data analysis pipeline.On the basis of the spatial multiomics technology,we propose a data integration strategy with cross-platform,cross-slice,and cross-modality.We summarize the potential applications of spatial monoomics technology,aiming to provide researchers and clinicians with a better understanding of how such applications have advanced.Spatial multiomics technology is expected to substantially impact biology and precision medicine through measurements of cellular tissue structures and the extraction of biomolecular features.展开更多
基金This work was supported by the National Key R&D Program of China(2021YFB3201202)the National Natural Science Foundation of China(nos.22005331,52275581,and 82372142)+3 种基金the Key Research and Development Program of Jiang su Province(nos.BE2022739,BE2020768,and SBE2022780090)the Youth Innovation Promotion Association of CAS(nos.2019322 and Y2022088)the Instrument Developing Project of the Chinese Academy of Sciences(nos.YJKYYQ20200046 and ZDKYYQ20210004)the Science and Technology Development Program of Suzhou(no.SJC2021019).
文摘Objective and Impact Statement:We describe an electroenzymatic mediator(EM)sensor based on an electroenzymatic assembly peak separation strategy,which can efficiently realize the simultaneous detection of 3 typical cardiovascular disease(CVD)metabolites in 5μl of plasma under one test.This work has substantial implications toward improving the efficiency of chronic CVD assessment.Introduction:Monitoring CVD of metabolites is strongly associated with disease risk.Independent and time-consuming detection in hospitals is unfavorable for chronic CVD management.Methods:The EM was flexibly designed by the cross-linking of electron mediators and enzymes,and 3 EM layers with different characteristics were assembled on one electrode.Electrons were transferred under tunable potential;3 metabolites were quantitatively detected by 3 peak currents that correlated with metabolite concentrations.Results:In this study,the EM sensor showed high sensitivity for the simultaneous detection of 3 metabolites with a lower limit of 0.01 mM.The linear correlation between the sensor and clinical was greater than 0.980 for 242 patients,and the consistency of risk assessment was 94.6%.Conclusion:Metabolites could be expanded by the EM,and the sensor could be a promising candidate as a home healthcare tool for CVD risk assessment.
基金This work was supported by the National Key R&D Program of China(No.2021YFC2500401)the National Natural Science Foundation of China(No.61874133,No.61901469,No.22005331)+4 种基金the Key Research and Development Program of Jiangsu Province(No.BE2018080,No.BE2019684,No.BE2020768)the Jihua Laboratory Foundation(No.X190181TD190)the Youth Innovation Promotion Association of CAS(No.2019322,No.2018360,No.Y201856)the Instrument Developing Project of the Chinese Academy of Sciences(No.YJKYYQ20190057,No.YJKYYQ20200046,No.ZDKYYQ20210004)the Science and Technology Development Program of Suzhou(No.SYG201907).
文摘Circulating tumor cells(CTCs)have tremendous potential to indicate disease progression and monitor therapeutic response using minimally invasive approaches.Considering the limitations of affinity strategies based on their cost,effectiveness,and simplicity,size-based enrichment methods that involve low-cost,label-free,and relatively simple protocols have been further promoted.Nevertheless,the key challenges of these methods are clogging issues and cell aggregation,which reduce the recovery rates and purity.Inspired by the natural phenomenon that the airflow around a windmill is disturbed,in this study,a windmill-like hole array on the SU-8 membrane was designed to perturb the fluid such that cells in a fluid would be able to self-mix and that the pressure acting on cells or the membrane would be dispersed to allow a greater velocity.In addition,based on the advantages of fluid coatings,a lipid coating was used to modify the membrane surface to prevent cell aggregation and clogging of the holes.Under the optimal conditions,recovery rates of 93%and 90%were found for A549 and HeLa cells in a clinical simulation test of our platform with a CTC concentration of 20-100 cells per milliliter of blood.The white blood cell(WBC)depletion rate was 98.7%(n=15),and the CTC detection limit was less than 10 cells per milliliter of blood(n=6).Moreover,compared with conventional membrane filtration,the advantages of the proposed device for the rapid(2 mL/min)and efficient enrichment of CTCs without clogging were shown both experimentally and theoretically.Due to its advantages in the efficient,rapid,uniform,and clog-free enrichment of CTCs,our platform offers great potential for metastatic detection and therapy analyses.
基金supported by the National Key R&D Program of China(no.2022YFF1202000)the National Natural Science Foundation of China(nos.52275581,82372142,and 82327802)+3 种基金the Key Research and Development Program of Jiangsu Province(no.BE2022739)the Youth Innovation Promotion Association of CAS(no.Y2022088)the Science and Technology Development Program of Suzhou(nos.SYG2024116 and SJC2021019)the CAS Key Laboratory of Bio-medical Diagnostics(no.A2023F001).
文摘Spatial monoomics has been recognized as a powerful tool for exploring life sciences.Recently,spatial multiomics has advanced considerably,which could contribute to clarifying many biological issues.Spatial monoomics techniques in epigenomics,genomics,transcriptomics,proteomics,and metabolomics can enhance our understanding of biological functions and cellular identities by simultaneously measuring tissue structures and biomolecule levels.Spatial monoomics technology has evolved from monoomics to spatial multiomics.Moreover,the spatial resolution,high-throughput detection capability,capture efficiency,and compatibility with various sample types of omics technology have considerably advanced.Despite the technological advances in this field,data analysis frameworks have stagnated.Current challenges include incomplete spatial monoomics data analysis pipeline,overly complex data analysis tasks,and few established spatial multiomics data analysis strategies.In this review,we systematically summarize recent developments of various spatial monoomics techniques and improvements in related data analysis pipeline.On the basis of the spatial multiomics technology,we propose a data integration strategy with cross-platform,cross-slice,and cross-modality.We summarize the potential applications of spatial monoomics technology,aiming to provide researchers and clinicians with a better understanding of how such applications have advanced.Spatial multiomics technology is expected to substantially impact biology and precision medicine through measurements of cellular tissue structures and the extraction of biomolecular features.
文摘针对沙漠、高原等降水稀少的极端天气,农业发展深受环境制约.然而,科学利用空气水与太阳能,能够有效缓解农作物产量不足的问题.本文通过Fe 3d电子空轨道与N、O孤电子对配位,形成吸水活性位点,从而构建吸湿凝胶,快速捕获空气水分子.实验发现,在15℃和60%相对湿度下,凝胶可以捕获自身重量1.16倍的水分子,且在40℃实现全脱水,能够稳定工作>60次吸附/解吸循环.高湿度环境下,凝胶的空气水捕获容量达3.5 g/g,证明了通过配位反应制备的凝胶能够快速捕获空气中的水分子,吸水效率远大于商业干燥剂,如硅胶与氯化钙.利用凝胶收集空气水,其液态水中各离子浓度达WHO饮用水标准,证明利用凝胶捕获的空气水可用于农业灌溉.利用吸湿凝胶设计农业自动补水装置,结合温度传感器、湿度传感器和光敏传感器对棚内环境进行实时监测,从而自动化控制棚内植物生长的环境.实验结果表明,利用凝胶能够将棚内的相对湿度从55%提升至88%,从而达到豌豆种子正常生长的环境,在干旱地区通过空气水自动灌溉后,豌豆苗持续生长>30 cm.
