Many landscape plants have edible,medicinal,forage,industrial and other economic value,which has not been fully exploited.Effective development and utilization of the economic value of city landscape plant,conforms to...Many landscape plants have edible,medicinal,forage,industrial and other economic value,which has not been fully exploited.Effective development and utilization of the economic value of city landscape plant,conforms to the need of sustainable development and economical society,which is of great significance to the development of urban ecological civilization construction. In this paper,the landscape plant species in Shijiazhuang City and their economic value are investigated and analyzed,the principle and scheme of economic development and utilization of landscape plants in Shijiazhuang city value are put forward,which provides the reference for full and sustainable utilization of landscape plant resources in Shijiazhuang City.展开更多
Objective:Microcirculatory disturbance is pathologically critical to acute pancreatitis(AP),which can be effectively alleviated by traditional Chinese medicine(TCM)formulas that activate blood flow.However,there has b...Objective:Microcirculatory disturbance is pathologically critical to acute pancreatitis(AP),which can be effectively alleviated by traditional Chinese medicine(TCM)formulas that activate blood flow.However,there has been no evidence-based research to date.Therefore,a well-designed systematic review and meta-analysis is necessary to elucidate the therapeutic transformative benefit of improving microcirculation during AP.This study aims to confirm the therapeutic efficacy of TCM formulas and explore the potential mechanisms underlying their effects on AP treatment.Methods:Studies from eight databases including Pubmed,Embase,Web of Science,Cochrane Library,CNKI,CBM,Wanfang,and Chinese VIP,were screened for the eligible randomized controlled trials(RCTs).The APACHEⅡscore and effectiveness rate were set as primary outcomes,while mortality rate,complications,total hospital stays,serum amylase recovery time,the time until the disappearance of abdominal pain,microcirculation indicators,and inflammation indicators were chosen as secondary outcomes.A systematic review and meta-analysis were subsequently conducted.Network pharmacology analysis was performed to analyze potential bioactive components with relevant targets of the core herbs included in the TCM formulas for activating blood flow.Results:A total of 51 RCTs(n=3721)were included.Compared with conventional western medical treatments alone,TCM groups were associated with lower APACHEⅡscore(SMD=1.36,95%CI:2.01 to 0.71,P=0.000)and higher effectiveness rate(RR:1.22,95%CI:1.18 to 1.26,P=0.000).Furthermore,the formulas for activating blood flow demonstrated significant efficacy in improving both microcirculation and inflammation indicators.Additionally,six core Chinese herbal medicines including Rhei Radix et Rhizoma with the highest frequency,Aurantii Fructus Immaturus,Paeoniae Radix Rubra,Bupleuri Radix,Salviae Miltiorrhizae Radix et Rhizoma,and Corydalis Rhizoma were filtered out from the adopted TCM formulas.Finally,166 shared targets between the six herbs and AP were identified.KEGG analysis indicated that lipid and atherosclerosis pathway is highly related to microcirculation.Conclusion:TCM formulas for activating blood flow significantly improve microcirculation and alleviate AP.Further high-quality,well-designed RCTs and deep mechanism exploration are required.展开更多
Acute pancreatitis(AP)is a life-threatening gastrointestinal disorder for which no effective pharmacological treatments are currently available.One of the pharmacological targets that merits further research is the ne...Acute pancreatitis(AP)is a life-threatening gastrointestinal disorder for which no effective pharmacological treatments are currently available.One of the pharmacological targets that merits further research is the neurokinin 1 receptor(NK1R),which is found on pancreatic acinar cells and responds to the neuropeptide substance P(SP)that participates in AP.Although a few studies have stated the involvement of SP/NK1R in neurogenic inflammation in AP development,the regulatory mechanism remains unclear.In this study,we found that following activation of NK1R by SP,b-arrestin1,a scaffold protein of NK1R,down-regulated transcription of Adss,Adsl,and Ampd in the purine nucleotide cycle,thereby inhibiting mitochondrial function through fumarate depletion.Interestingly,we identified magnolol as a new and natural NK1R inhibitor with a non-nitrogenous biphenyl core structure.It exhibited a beneficial effect on AP by restoring purine nucleotide cycle metabolic enzymes and fumarate levels.Our study not only provides new therapeutic strategies,leading compounds,and drug translation possibilities for AP,but also provides important clues for the study of downstream mechanisms driven by SP in other diseases.展开更多
Acute pancreatitis(AP)is a potentially fatal condition with no targeted treatment options.Although inhibiting xanthine oxidase(XO)in the treatment of AP has been studied in several experimental models and clinical tri...Acute pancreatitis(AP)is a potentially fatal condition with no targeted treatment options.Although inhibiting xanthine oxidase(XO)in the treatment of AP has been studied in several experimental models and clinical trials,whether XO is a target of AP and what its the main mechanism of action is remains unclear.Here,we aimed to re-evaluate whether XO is a target aggravating AP other than merely generating reactive oxygen species that trigger AP.We first revealed that XO expression and enzyme activity were significantly elevated in the serum and pancreas of necrotizing AP models.We also found that allopurinol and febuxostat,as purine-like and non-purine XO inhibitors,respectively,exhibited protective effects against pancreatic acinar cell death in vitro and pancreatic damage in vivo at different doses and treatment time points.Moreover,we observed that conditional Xdh overexpression aggravated pancreatic necrosis and severity.Further mechanism analysis showed that XO inhibition restored the hypoxia-inducible factor 1-alpha(HIF-1α)-regulated lactate dehydrogenase A(LDHA)and NOD-like receptor family pyrin domain containing 3(NLRP3)signaling pathways and reduced the enrichment of^(13)C_(6)-glucose to^(13)C_(3)-lactate.Lastly,we observed that clinical circulatory XO activity was significantly elevated in severe cases and correlated with C-reactive protein levels,while pancreatic XO and urate were also increased in severe AP patients.These results together indicated that proper inhibition of XO might be a promising therapeutic strategy for alleviating pancreatic necrosis and preventing progression of severe AP by downregulating HIF-1α-mediated LDHA and NLRP3 signaling pathways.展开更多
基金Supported by Funds for the Development of Key Disciplines in Hebei UniversitiesUniversity-level Platform Research Project of Shijiazhuang University in 2013(XJPT011)
文摘Many landscape plants have edible,medicinal,forage,industrial and other economic value,which has not been fully exploited.Effective development and utilization of the economic value of city landscape plant,conforms to the need of sustainable development and economical society,which is of great significance to the development of urban ecological civilization construction. In this paper,the landscape plant species in Shijiazhuang City and their economic value are investigated and analyzed,the principle and scheme of economic development and utilization of landscape plants in Shijiazhuang city value are put forward,which provides the reference for full and sustainable utilization of landscape plant resources in Shijiazhuang City.
基金supported by National Natural Science Foundation of China(No.82274321 to Q.X.,No.82374256 to D.D.,No.82104598 to C.H.)the Sichuan Provincial Natural Science Foundation(No.2022NSFSC0641 to Q.X.)。
文摘Objective:Microcirculatory disturbance is pathologically critical to acute pancreatitis(AP),which can be effectively alleviated by traditional Chinese medicine(TCM)formulas that activate blood flow.However,there has been no evidence-based research to date.Therefore,a well-designed systematic review and meta-analysis is necessary to elucidate the therapeutic transformative benefit of improving microcirculation during AP.This study aims to confirm the therapeutic efficacy of TCM formulas and explore the potential mechanisms underlying their effects on AP treatment.Methods:Studies from eight databases including Pubmed,Embase,Web of Science,Cochrane Library,CNKI,CBM,Wanfang,and Chinese VIP,were screened for the eligible randomized controlled trials(RCTs).The APACHEⅡscore and effectiveness rate were set as primary outcomes,while mortality rate,complications,total hospital stays,serum amylase recovery time,the time until the disappearance of abdominal pain,microcirculation indicators,and inflammation indicators were chosen as secondary outcomes.A systematic review and meta-analysis were subsequently conducted.Network pharmacology analysis was performed to analyze potential bioactive components with relevant targets of the core herbs included in the TCM formulas for activating blood flow.Results:A total of 51 RCTs(n=3721)were included.Compared with conventional western medical treatments alone,TCM groups were associated with lower APACHEⅡscore(SMD=1.36,95%CI:2.01 to 0.71,P=0.000)and higher effectiveness rate(RR:1.22,95%CI:1.18 to 1.26,P=0.000).Furthermore,the formulas for activating blood flow demonstrated significant efficacy in improving both microcirculation and inflammation indicators.Additionally,six core Chinese herbal medicines including Rhei Radix et Rhizoma with the highest frequency,Aurantii Fructus Immaturus,Paeoniae Radix Rubra,Bupleuri Radix,Salviae Miltiorrhizae Radix et Rhizoma,and Corydalis Rhizoma were filtered out from the adopted TCM formulas.Finally,166 shared targets between the six herbs and AP were identified.KEGG analysis indicated that lipid and atherosclerosis pathway is highly related to microcirculation.Conclusion:TCM formulas for activating blood flow significantly improve microcirculation and alleviate AP.Further high-quality,well-designed RCTs and deep mechanism exploration are required.
基金supported by the National Natural Science Foundation of China(No.82104598 to Chenxia Han,No.82274321 to Qing Xia,No.82170905 to Dan Du)Project of Sichuan Provincial Administration of Traditional Chinese Medicine(2023ZD04 to Qing Xia,China).
文摘Acute pancreatitis(AP)is a life-threatening gastrointestinal disorder for which no effective pharmacological treatments are currently available.One of the pharmacological targets that merits further research is the neurokinin 1 receptor(NK1R),which is found on pancreatic acinar cells and responds to the neuropeptide substance P(SP)that participates in AP.Although a few studies have stated the involvement of SP/NK1R in neurogenic inflammation in AP development,the regulatory mechanism remains unclear.In this study,we found that following activation of NK1R by SP,b-arrestin1,a scaffold protein of NK1R,down-regulated transcription of Adss,Adsl,and Ampd in the purine nucleotide cycle,thereby inhibiting mitochondrial function through fumarate depletion.Interestingly,we identified magnolol as a new and natural NK1R inhibitor with a non-nitrogenous biphenyl core structure.It exhibited a beneficial effect on AP by restoring purine nucleotide cycle metabolic enzymes and fumarate levels.Our study not only provides new therapeutic strategies,leading compounds,and drug translation possibilities for AP,but also provides important clues for the study of downstream mechanisms driven by SP in other diseases.
基金supported by the National Natural Science Foundation of China(Dan Du,82170905)the Program of Science and Technology Department of Sichuan Province(Dan Du,2023NSFSC1755,China)+2 种基金the State Key Laboratory of Bioactive Substance and Function of Natural Medicines,Institute of Materia Medica,Chinese Academy of Medical Sciences and Peking Union Medical College(Dan Du,GTZK202107,China)the 1.3.5 Project for Disciplines of Excellence,West China Hospital,Sichuan University(Qing Xia,ZYJC18005,China)the West China,Nursing Discipline Development Special Fund Project,Sichuan University(Xia Li,HXHL21060,China).
文摘Acute pancreatitis(AP)is a potentially fatal condition with no targeted treatment options.Although inhibiting xanthine oxidase(XO)in the treatment of AP has been studied in several experimental models and clinical trials,whether XO is a target of AP and what its the main mechanism of action is remains unclear.Here,we aimed to re-evaluate whether XO is a target aggravating AP other than merely generating reactive oxygen species that trigger AP.We first revealed that XO expression and enzyme activity were significantly elevated in the serum and pancreas of necrotizing AP models.We also found that allopurinol and febuxostat,as purine-like and non-purine XO inhibitors,respectively,exhibited protective effects against pancreatic acinar cell death in vitro and pancreatic damage in vivo at different doses and treatment time points.Moreover,we observed that conditional Xdh overexpression aggravated pancreatic necrosis and severity.Further mechanism analysis showed that XO inhibition restored the hypoxia-inducible factor 1-alpha(HIF-1α)-regulated lactate dehydrogenase A(LDHA)and NOD-like receptor family pyrin domain containing 3(NLRP3)signaling pathways and reduced the enrichment of^(13)C_(6)-glucose to^(13)C_(3)-lactate.Lastly,we observed that clinical circulatory XO activity was significantly elevated in severe cases and correlated with C-reactive protein levels,while pancreatic XO and urate were also increased in severe AP patients.These results together indicated that proper inhibition of XO might be a promising therapeutic strategy for alleviating pancreatic necrosis and preventing progression of severe AP by downregulating HIF-1α-mediated LDHA and NLRP3 signaling pathways.
