Melanoma is characterized by high malignancy,ranking the third among skin malignancies,and is associated with lack of specific treatment options and poor prognosis.Therefore,the development of effective therapies for ...Melanoma is characterized by high malignancy,ranking the third among skin malignancies,and is associated with lack of specific treatment options and poor prognosis.Therefore,the development of effective therapies for melanoma is imperative.A critical challenge in addressing subcutaneous disease lies in overcoming the skin barrier.In this study,we engineered a microneedle(MN)system that in-tegrates chemotherapy,photothermal therapy(PTT),and targeted therapy to enhance anti-tumor effi-cacy while effectively penetrating the skin barrier.In vitro studies have demonstrated that the MN drug delivery system(DDS)can effectively penetrate the stratum corneum of the skin,deliver therapeutics to subcutaneous tumor sites,and establish a drug reservoir at these locations to exert anti-tumor effects.Cellular experiments indicated that the engineered PTT chemotherapy-targeted MNs can be internalized by tumor cells,exhibiting enhanced cytotoxicity against them.In vivo pharmacological investigations revealed that the combination of PITT and chemotherapy delivered via this MN DDS produced synergistic anti-tumor effects,achieving a tumor inhibition rate of up to 98.15%.This in situ DDS minimizes involvement with other organs,significantly reducing chemotherapy-related side effects.In summary,the PTT chemotherapy-targeted MNs developed in this study demonstrate promising application po-tential by enhancing anti-tumor efficacy while minimizing adverse effects.展开更多
基金the Natural Science Foundation of Xiamen,China(Grant No.:3502z20227074)NaturalScience Foundation of Fujian Province,China(Grant Nos.:2022J011405, 2022J05320)+1 种基金Fujian College Student Innovation and Entrepreneurship Training Program(Program No.:202312631008)National College Student Innovation and Entrepreneurship Training Program(Program No.:202312631003).
文摘Melanoma is characterized by high malignancy,ranking the third among skin malignancies,and is associated with lack of specific treatment options and poor prognosis.Therefore,the development of effective therapies for melanoma is imperative.A critical challenge in addressing subcutaneous disease lies in overcoming the skin barrier.In this study,we engineered a microneedle(MN)system that in-tegrates chemotherapy,photothermal therapy(PTT),and targeted therapy to enhance anti-tumor effi-cacy while effectively penetrating the skin barrier.In vitro studies have demonstrated that the MN drug delivery system(DDS)can effectively penetrate the stratum corneum of the skin,deliver therapeutics to subcutaneous tumor sites,and establish a drug reservoir at these locations to exert anti-tumor effects.Cellular experiments indicated that the engineered PTT chemotherapy-targeted MNs can be internalized by tumor cells,exhibiting enhanced cytotoxicity against them.In vivo pharmacological investigations revealed that the combination of PITT and chemotherapy delivered via this MN DDS produced synergistic anti-tumor effects,achieving a tumor inhibition rate of up to 98.15%.This in situ DDS minimizes involvement with other organs,significantly reducing chemotherapy-related side effects.In summary,the PTT chemotherapy-targeted MNs developed in this study demonstrate promising application po-tential by enhancing anti-tumor efficacy while minimizing adverse effects.
