Introduction:Addressing the healthcare needs of the growing number of patients with herpes zoster(HZ)presents considerable challenges.This study examined the unmet healthcare needs and associated factors among people ...Introduction:Addressing the healthcare needs of the growing number of patients with herpes zoster(HZ)presents considerable challenges.This study examined the unmet healthcare needs and associated factors among people with HZ across 25 provincial-level administrative divisions(PLADs)and cities in China from 2019–2024.Methods:The study conducted a nationwide population-based survey using probabilityproportional-to-size sampling.The structured questionnaire incorporated validated diagnostic criteria for HZ,HZ health service utilization-related questions,and sociodemographic information.Unmet healthcare needs were categorized as unmet outpatient and hospitalization care needs.Odds ratio(OR)was estimated from multivariable logistic regression modeling to assess the associations between HZ unmet healthcare needs and sociodemographic characteristics of individuals.This study ultimately completed 21,750 adult surveys(≥16 years old)in 25 PLADs across the country.Among them,a healthcare utilization survey was conducted for patients who had developed HZ in the past five years of the survey period,totaling 813 individuals.Results:Among 813 participants with HZ,28.78%had unmet outpatient care needs,and 36.36%of those who met the hospitalization criteria had unmet inpatient needs.Older adults had significantly lower odds of unmet outpatient needs,with an OR of 0.57[95%confidence interval(CI):0.40,0.81],compared with younger adults.In addition,urban residents had higher odds of unmet inpatient needs,with an OR of 2.18(95%CI:1.00–4.75).Conclusions:The findings indicate substantial unmet healthcare needs among patients with HZ,with significant variations observed across different age groups and residential regions.This study highlights the necessity of enhancing health literacy,with particular emphasis on improving the standardized diagnosis of HZ in rural areas and the standardized treatment among working-age residents with HZ.展开更多
We report an efficient and convergent strategy for the total synthesis of UCS1025A and its diastereomer tetra-epi-UCS1025A.Ucs1025A is a representative member of the naturally occurring pyrrolizidinone polyketides,fro...We report an efficient and convergent strategy for the total synthesis of UCS1025A and its diastereomer tetra-epi-UCS1025A.Ucs1025A is a representative member of the naturally occurring pyrrolizidinone polyketides,from which members with potent antibacterial,antifungal,and anticancer activities have been identified.Our approach features a tandem carbonylative Stille cross coupling and Diels-Alder reaction to forge a key C-C bond and build the trans-decalin system.This tandem process utilizes carbon monoxide as a one-carbon linchpin to stitch a vinyl triflate and a vinylstannane together and form the desired enone moiety for the subsequent intramolecular Diels-Alder cyclization.Our synthesis also provides a versatile approach for the synthesis of other related pyrrolizidinone-containing polyketides.展开更多
Microbial cell factories(MCFs)and cell-free systems(CFSs)are generally considered as two unrelated approaches for the biosynthesis of biomolecules.In the current study,two systems were combined together for the overpr...Microbial cell factories(MCFs)and cell-free systems(CFSs)are generally considered as two unrelated approaches for the biosynthesis of biomolecules.In the current study,two systems were combined together for the overproduction of agroclavine(AC),a structurally complex ergot alkaloid.The whole biosynthetic pathway for AC was split into the early pathway and the late pathway at the point of the FAD-linked oxidoreductase EasE,which was reconstituted in an MCF(Aspergillus nidulans)and a four-enzyme CFS,respectively.The final titer of AC of this combined system is 1209 mg/L,which is the highest one that has been reported so far,to the best of our knowledge.The development of such a combined route could potentially avoid the limitations of both MCF and CFS systems,and boost the production of complex ergot alkaloids with polycyclic ring systems.展开更多
文摘Introduction:Addressing the healthcare needs of the growing number of patients with herpes zoster(HZ)presents considerable challenges.This study examined the unmet healthcare needs and associated factors among people with HZ across 25 provincial-level administrative divisions(PLADs)and cities in China from 2019–2024.Methods:The study conducted a nationwide population-based survey using probabilityproportional-to-size sampling.The structured questionnaire incorporated validated diagnostic criteria for HZ,HZ health service utilization-related questions,and sociodemographic information.Unmet healthcare needs were categorized as unmet outpatient and hospitalization care needs.Odds ratio(OR)was estimated from multivariable logistic regression modeling to assess the associations between HZ unmet healthcare needs and sociodemographic characteristics of individuals.This study ultimately completed 21,750 adult surveys(≥16 years old)in 25 PLADs across the country.Among them,a healthcare utilization survey was conducted for patients who had developed HZ in the past five years of the survey period,totaling 813 individuals.Results:Among 813 participants with HZ,28.78%had unmet outpatient care needs,and 36.36%of those who met the hospitalization criteria had unmet inpatient needs.Older adults had significantly lower odds of unmet outpatient needs,with an OR of 0.57[95%confidence interval(CI):0.40,0.81],compared with younger adults.In addition,urban residents had higher odds of unmet inpatient needs,with an OR of 2.18(95%CI:1.00–4.75).Conclusions:The findings indicate substantial unmet healthcare needs among patients with HZ,with significant variations observed across different age groups and residential regions.This study highlights the necessity of enhancing health literacy,with particular emphasis on improving the standardized diagnosis of HZ in rural areas and the standardized treatment among working-age residents with HZ.
文摘We report an efficient and convergent strategy for the total synthesis of UCS1025A and its diastereomer tetra-epi-UCS1025A.Ucs1025A is a representative member of the naturally occurring pyrrolizidinone polyketides,from which members with potent antibacterial,antifungal,and anticancer activities have been identified.Our approach features a tandem carbonylative Stille cross coupling and Diels-Alder reaction to forge a key C-C bond and build the trans-decalin system.This tandem process utilizes carbon monoxide as a one-carbon linchpin to stitch a vinyl triflate and a vinylstannane together and form the desired enone moiety for the subsequent intramolecular Diels-Alder cyclization.Our synthesis also provides a versatile approach for the synthesis of other related pyrrolizidinone-containing polyketides.
基金This study was supported by the National Key Research and Development Program of China(grant nos.2021YFC2100600,2019YFA0905100 and 2018YFA0901600)the National Natural Science Foundation of China(grant nos.31872614,32022002,21977113)+1 种基金the Youth Scientists Innovation Promotion Association of CAS(2019090)to S.S.G.,Innovative Cross Team project of Chinese Academy of Sciences,CAS(grant no.JCTD-2019-06)Shandong Provincial Natural Science Foundation(Major Basic Research Projects)(grant no.ZR2019ZD18).
文摘Microbial cell factories(MCFs)and cell-free systems(CFSs)are generally considered as two unrelated approaches for the biosynthesis of biomolecules.In the current study,two systems were combined together for the overproduction of agroclavine(AC),a structurally complex ergot alkaloid.The whole biosynthetic pathway for AC was split into the early pathway and the late pathway at the point of the FAD-linked oxidoreductase EasE,which was reconstituted in an MCF(Aspergillus nidulans)and a four-enzyme CFS,respectively.The final titer of AC of this combined system is 1209 mg/L,which is the highest one that has been reported so far,to the best of our knowledge.The development of such a combined route could potentially avoid the limitations of both MCF and CFS systems,and boost the production of complex ergot alkaloids with polycyclic ring systems.
