Oxysophoridine, a new alkaloid extracted from Sophora alopecuroides L., has been shown to have a protective effect against ischemic brain damage. In this study, a focal cerebral ischemia/reperfusion injury model was e...Oxysophoridine, a new alkaloid extracted from Sophora alopecuroides L., has been shown to have a protective effect against ischemic brain damage. In this study, a focal cerebral ischemia/reperfusion injury model was established using middle cerebral artery occlusion in mice. Both 62.5, 125, and 250 mg/kg oxysophoridine, via intraperitoneal injection, and 6 mg/kg nimodipine, via intragastric administration, were administered daily for 7 days before modeling. After 24 hours of reperfusion, mice were tested for neurological deficit, cerebral infarct size was assessed and brain tissue was collected. Results showed that oxysophoridine at 125, 250 mg/kg and 6 mg/kg nimodipine could reduce neurological deficit scores, cerebral infarct size and brain water content in mice. These results provided evidence that oxysophoridine plays a protective role in cerebral ischemia/reperfusion injury. In addition, oxysophoridine at 62.5, 125, and 250 mg/kg and 6 mg/kg nimodipine increased adenosine-triphosphate content, and decreased malondialdehyde and nitric oxide content. These compounds enhanced the activities of glutathione-peroxidase, superoxide dismutase, catalase, and lactate dehydrogenase, and decreased the activity of nitric oxide synthase Protein and mRNA expression levels of N-methyI-D-aspartate receptor subunit NR1 were markedly inhibited in the presence of 250 mg/kg oxysophoridine and 6 mg/kg nimodipine. Our experimental findings indicated that oxysophoridine has a neuroprotective effect against cerebral ischemia/reperfusion injury in mice, and that the effect may be due to its ability to inhibit oxidative stress and expression of the N-methyI-D-aspartate receptor subunit NR1.展开更多
Tropical crops play a pivotal role in ensuring global food security and driving sustainable agricultural develop-ment,particularly in regions with limited resources.However,the complexity of tropical crop genomes and ...Tropical crops play a pivotal role in ensuring global food security and driving sustainable agricultural develop-ment,particularly in regions with limited resources.However,the complexity of tropical crop genomes and the lack of integrated omics data pose significant challenges to genetic improvement and biodesign research.Tro-piCODB is a comprehensive multi-omics database designed to address these challenges by integrating high-quality genomic,variome,transcriptomic,metabolomic,and phenotypic datasets for eight economically significant tropical crops,including cassava,sugarcane,and oil palm.Advanced tools such as gene family analysis,miRNA profiling,and precision guideRNA design are seamlessly incorporated,offering unprecedented support for functional genomics and molecular breeding.TropiCODB's user-friendly interface and dynamic visualization capabilities enable researchers to efficiently explore key genetic mechanisms and design targeted breeding strategies.By leveraging innovative technologies and curated datasets,TropiCODB provides a robust foundation for advancing tropical agriculture through precision biodesign.展开更多
基金supported by the National Natural Science Foundation of China, No. 30960506, 81160524the Natural Science Foundation of Ningxia Hui Autonomous Region, No. NZ11212+1 种基金the Key Scientific Research Project of Ningxia Hui Autonomous Region Health Department, No. 2012152the Project of Ningxia Medical University, No. XM2011017
文摘Oxysophoridine, a new alkaloid extracted from Sophora alopecuroides L., has been shown to have a protective effect against ischemic brain damage. In this study, a focal cerebral ischemia/reperfusion injury model was established using middle cerebral artery occlusion in mice. Both 62.5, 125, and 250 mg/kg oxysophoridine, via intraperitoneal injection, and 6 mg/kg nimodipine, via intragastric administration, were administered daily for 7 days before modeling. After 24 hours of reperfusion, mice were tested for neurological deficit, cerebral infarct size was assessed and brain tissue was collected. Results showed that oxysophoridine at 125, 250 mg/kg and 6 mg/kg nimodipine could reduce neurological deficit scores, cerebral infarct size and brain water content in mice. These results provided evidence that oxysophoridine plays a protective role in cerebral ischemia/reperfusion injury. In addition, oxysophoridine at 62.5, 125, and 250 mg/kg and 6 mg/kg nimodipine increased adenosine-triphosphate content, and decreased malondialdehyde and nitric oxide content. These compounds enhanced the activities of glutathione-peroxidase, superoxide dismutase, catalase, and lactate dehydrogenase, and decreased the activity of nitric oxide synthase Protein and mRNA expression levels of N-methyI-D-aspartate receptor subunit NR1 were markedly inhibited in the presence of 250 mg/kg oxysophoridine and 6 mg/kg nimodipine. Our experimental findings indicated that oxysophoridine has a neuroprotective effect against cerebral ischemia/reperfusion injury in mice, and that the effect may be due to its ability to inhibit oxidative stress and expression of the N-methyI-D-aspartate receptor subunit NR1.
基金supported by Hainan Province Science and Technology Special Fund(ZDYF2023XDNY050)National Key Laboratory for Tropical Crop Breeding(NO.NKLTCB202337)Hainan Province Graduate Student Innovation Research Project(Qhys2023-268).
文摘Tropical crops play a pivotal role in ensuring global food security and driving sustainable agricultural develop-ment,particularly in regions with limited resources.However,the complexity of tropical crop genomes and the lack of integrated omics data pose significant challenges to genetic improvement and biodesign research.Tro-piCODB is a comprehensive multi-omics database designed to address these challenges by integrating high-quality genomic,variome,transcriptomic,metabolomic,and phenotypic datasets for eight economically significant tropical crops,including cassava,sugarcane,and oil palm.Advanced tools such as gene family analysis,miRNA profiling,and precision guideRNA design are seamlessly incorporated,offering unprecedented support for functional genomics and molecular breeding.TropiCODB's user-friendly interface and dynamic visualization capabilities enable researchers to efficiently explore key genetic mechanisms and design targeted breeding strategies.By leveraging innovative technologies and curated datasets,TropiCODB provides a robust foundation for advancing tropical agriculture through precision biodesign.
