Hexavalent chromium Cr(Ⅵ),as a well-established carcinogen,contributes to tumorigenesis for many human cancers,especially respiratory and digestive tumors.However,the potential function and relevant mechanism of Cr(...Hexavalent chromium Cr(Ⅵ),as a well-established carcinogen,contributes to tumorigenesis for many human cancers,especially respiratory and digestive tumors.However,the potential function and relevant mechanism of Cr(Ⅵ)on the initiation of esophageal carcinogenesis are largely unknown.Here,immortalized human esophageal epithelial cells(HEECs)were induced to be malignantly transformed cells,termed HEEC-Cr(Ⅵ)cells,via chronic exposure to Cr(Ⅵ),which simulates the progress of esophageal tumorigenesis.In vitro and in vivo experiments demonstrated that HEEC-Cr(Ⅵ)cells obtain the ability of anchorage-independent growth,greater proliferative capacity,cancer stem cell properties,and the capacity to form subcutaneous xenografts in BALB/c nude mice when compared to their parental cells,HEECs.Additionally,HEEC-Cr(Ⅵ)cells exhibited weakened cell motility and enhanced cell adhesion.Interestingly,HEECs with acute exposure to Cr(Ⅵ)failed to display those malignant phenotypes of HEEC-Cr(Ⅵ)cells,suggesting that Cr(Ⅵ)-induced malignant transformation,but not Cr(Ⅵ)itself,is the cause for the tumor characteristics of HEEC-Cr(Ⅵ)cells.Mechanistically,chronic exposure to Cr(Ⅵ)induced abnormal activation of Notch signaling,which is crucial to maintaining the capacity for malignant proliferation and stemness of HEEC-Cr(Ⅵ)cells.As expected,N-[N-(3,5-difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester(DAPT),an inhibitor for the Notch pathway,drastically attenuated cancerous phenotypes of HEEC-Cr(Ⅵ)cells.In conclusion,our study clarified the molecular mechanism underlying Cr(Ⅵ)-induced esophageal tumorigenesis,which provides novel insights for further basic research and clinical therapeutic strategies about Cr(Ⅵ)-associated esophageal cancer.展开更多
The fundamental purpose of ideological and political education in colleges and universities is to inspire and enhance young students’ability to correctly understand and transform the world.College students born after...The fundamental purpose of ideological and political education in colleges and universities is to inspire and enhance young students’ability to correctly understand and transform the world.College students born after the 1990s are in a period of social transformation in China.The open and pluralistic society influences the formation of their moral,life,world,and value outlooks.A healthy young student needs both a healthy body and a healthy mind to adapt to social development and changes.By applying their psychological knowledge,the authors actively carry out mental health education for various problems troubling students,boldly innovate the working ideas of student management,and improve work efficiency.Practice has proved that mental health education is an effective approach to ideological and political education in colleges and universities.It is of great significance to use mental health education to expand and enrich the connotation of ideological and political work in colleges and universities.展开更多
This paper analyzes the characteristics of Interact space and confrontation, discussed on the main technology of network space attack and defense confrontation. The paper presents the realization scheme of network spa...This paper analyzes the characteristics of Interact space and confrontation, discussed on the main technology of network space attack and defense confrontation. The paper presents the realization scheme of network space attack defense confrontation system, and analyzes its feasibility. The technology and the system can provide technical support for the system in the network space of our country development, and safeguard security of network space in China, promote the development of the network space security industry of China, it plays an important role and significance to speed up China＇ s independent controllable security products development.展开更多
A novel series of polybenzazoles with rigid-rod benzoxazole cycle and soft methylene segment was designed and synthesized via solution condensation poly-merizations from 4,6-diamino-l,3-benzenediol dipho-sphate,tereph...A novel series of polybenzazoles with rigid-rod benzoxazole cycle and soft methylene segment was designed and synthesized via solution condensation poly-merizations from 4,6-diamino-l,3-benzenediol dipho-sphate,terephthalic acid and aliphatic dicarboxylic acid.The structures of polybenzazoles were characterized by means of FT-IR,1H NMR and Wide-angle X-ray diffrac-tion(WAXRD).All the polymers show excellent thermal stability and the TdS was above 471℃,The intrinsic vis-cosities[η]of the polymers ranged from 0.8 to 0.9.The UV-Vis absorption peaks of the polymers in MSA were blue-shifted from 429 nm for PBO to 291 nm for PBOC7,and the Stokes shifts in PL spectra enlarged.展开更多
基金supported by the Special Construction Project Fund for Taishan Mountain Scholars of Shandong Provincethe Jinan Medicine Research Program+1 种基金the Nurturing and Development Fund from The Second Hospital of Shandong University(No.2022YP62)the Shandong Provincial Natural Science Foundation for Young Scholars(No.ZR2022QH285),China.
文摘Hexavalent chromium Cr(Ⅵ),as a well-established carcinogen,contributes to tumorigenesis for many human cancers,especially respiratory and digestive tumors.However,the potential function and relevant mechanism of Cr(Ⅵ)on the initiation of esophageal carcinogenesis are largely unknown.Here,immortalized human esophageal epithelial cells(HEECs)were induced to be malignantly transformed cells,termed HEEC-Cr(Ⅵ)cells,via chronic exposure to Cr(Ⅵ),which simulates the progress of esophageal tumorigenesis.In vitro and in vivo experiments demonstrated that HEEC-Cr(Ⅵ)cells obtain the ability of anchorage-independent growth,greater proliferative capacity,cancer stem cell properties,and the capacity to form subcutaneous xenografts in BALB/c nude mice when compared to their parental cells,HEECs.Additionally,HEEC-Cr(Ⅵ)cells exhibited weakened cell motility and enhanced cell adhesion.Interestingly,HEECs with acute exposure to Cr(Ⅵ)failed to display those malignant phenotypes of HEEC-Cr(Ⅵ)cells,suggesting that Cr(Ⅵ)-induced malignant transformation,but not Cr(Ⅵ)itself,is the cause for the tumor characteristics of HEEC-Cr(Ⅵ)cells.Mechanistically,chronic exposure to Cr(Ⅵ)induced abnormal activation of Notch signaling,which is crucial to maintaining the capacity for malignant proliferation and stemness of HEEC-Cr(Ⅵ)cells.As expected,N-[N-(3,5-difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester(DAPT),an inhibitor for the Notch pathway,drastically attenuated cancerous phenotypes of HEEC-Cr(Ⅵ)cells.In conclusion,our study clarified the molecular mechanism underlying Cr(Ⅵ)-induced esophageal tumorigenesis,which provides novel insights for further basic research and clinical therapeutic strategies about Cr(Ⅵ)-associated esophageal cancer.
基金2022 Provincial Teaching Research Project of Hubei Undergraduate Universities“Innovation and Practice of Mental Health Education for Applied College Students from the Perspective of‘Three-All Education’”(2022530)2023 Provincial Teaching Reform Research Project of Hubei Undergraduate Universities“Research on the Construction and Teaching Reform of‘Energy-Saving and Environmental Protection’Landscape Architecture Major under the Background of National Strategic Emerging Industries Development”(2023584)2023 Special Funded General Project of Hubei Education Science Planning“Research on the Linkage Mechanism of‘Enrollment-Cultivation-Employment’for Pilot Majors in Local Universities under the Background of a Quality Power”(2023ZB010)。
文摘The fundamental purpose of ideological and political education in colleges and universities is to inspire and enhance young students’ability to correctly understand and transform the world.College students born after the 1990s are in a period of social transformation in China.The open and pluralistic society influences the formation of their moral,life,world,and value outlooks.A healthy young student needs both a healthy body and a healthy mind to adapt to social development and changes.By applying their psychological knowledge,the authors actively carry out mental health education for various problems troubling students,boldly innovate the working ideas of student management,and improve work efficiency.Practice has proved that mental health education is an effective approach to ideological and political education in colleges and universities.It is of great significance to use mental health education to expand and enrich the connotation of ideological and political work in colleges and universities.
文摘This paper analyzes the characteristics of Interact space and confrontation, discussed on the main technology of network space attack and defense confrontation. The paper presents the realization scheme of network space attack defense confrontation system, and analyzes its feasibility. The technology and the system can provide technical support for the system in the network space of our country development, and safeguard security of network space in China, promote the development of the network space security industry of China, it plays an important role and significance to speed up China＇ s independent controllable security products development.
文摘A novel series of polybenzazoles with rigid-rod benzoxazole cycle and soft methylene segment was designed and synthesized via solution condensation poly-merizations from 4,6-diamino-l,3-benzenediol dipho-sphate,terephthalic acid and aliphatic dicarboxylic acid.The structures of polybenzazoles were characterized by means of FT-IR,1H NMR and Wide-angle X-ray diffrac-tion(WAXRD).All the polymers show excellent thermal stability and the TdS was above 471℃,The intrinsic vis-cosities[η]of the polymers ranged from 0.8 to 0.9.The UV-Vis absorption peaks of the polymers in MSA were blue-shifted from 429 nm for PBO to 291 nm for PBOC7,and the Stokes shifts in PL spectra enlarged.
