[Objectives]This study was conducted to explore the physiological mechanism of flower color variation in the white mutants of Anthurium andeaeanum.[Methods]The seven white mutants of'Alabama'and'Turenza...[Objectives]This study was conducted to explore the physiological mechanism of flower color variation in the white mutants of Anthurium andeaeanum.[Methods]The seven white mutants of'Alabama'and'Turenza'were used as materials to analyze the pigment types,flavonoid types and content and anthocyanin content in the wild type and mutants.[Results]The white spathe mainly contained flavonoids,flavonols,dihydroflavonols and dihydroflavonols;the white mutants of'Alabama'had a higher total flavonoid content than the wild type,while the white mutants of'Turenza'showed an opposite trend;and the spathe of the wild type had the highest anthocyanin content,and the pink part of the two-color mutant or the spathe of the binary color mutant contained trace anthocyanins,while no anthocyanins were detected in the white part of the mutants.[Conclusions]The main cause of the white mutants of A.andeaeanum is related to anthocyanin metabolism.展开更多
[Objectives] This study was conducted to investigate the effect of paclobutrazol （PBZ） on heat tolerance of dahlia.[Methods] A dahlia variety Danbanhuang was selected as the experimental material in this study. Afte...[Objectives] This study was conducted to investigate the effect of paclobutrazol （PBZ） on heat tolerance of dahlia.[Methods] A dahlia variety Danbanhuang was selected as the experimental material in this study. After 100, 200, 300 or 400 mg/L paclobutrazol was sprayed evenly to the leaves of dahlia seedlings, they were cultured in an incubator at high temperature （35 ℃/30 ℃, day/night） for two days, and then transferred to an incubator at normal temperature （25 ℃/20 ℃, day/night）. The physiological and biochemical parameters of the dahlia seedlings were measured before exposure to high temperature （D0）, after exposure to high temperature for two days （D2） and exposure to normal temperature for one day （R1）.[Results] Compared to the negative control, foliar application of paclobutrazol decreased the content of MDA, increased the contents of chlorophyll, proline, soluble protein, and the activity of SOD, POD and CAT in dahlia plants under heat stress. The contents of MDA, soluble protein and proline in PBZ treated dahlia plants increased when they were subject to high temperature stress （35 ℃/30 ℃, day/night）, and then decreased when the temperature returned to normal （25 ℃/20 ℃, day/night）, and CAT activity decreased at high temperature, and then increased at normal temperature, while the activity of SOD and POD kept rising during the entire experimental period.[Conclusions] Foliar application of paclobutrazol can alleviate the adverse effect caused by high temperature to dahlia plants, and the experimental data provide a theoretical basis for the application of paclobutrazol in dahlia cultivation in South China.展开更多
Objectives] This study was conducted to investigate the regulatory mechanism of ethylene on DpXTH gene expression in dahlia （ Dahlia pinnata Cav.） flower petals.[Methods] Dahlia flower petals were treated with ethep...Objectives] This study was conducted to investigate the regulatory mechanism of ethylene on DpXTH gene expression in dahlia （ Dahlia pinnata Cav.） flower petals.[Methods] Dahlia flower petals were treated with ethephon （ETH） and 1-methylcyclopropene （1-MCP） at different flower development stages and for different durations in vivo . Then, the relative expression of DpXTH1 and DpXTH2 in flower samples in different treatments was quantified with real-time PCR, using β-actin as a reference gene.[Results] The expression of DpXTH1 and DpXTH2 in ETH- or 1-MCP-treated dahlia flower petals increased at first, and decreased subsequently with increasing duration of treatment. The relative expression of both DpXTH1 and DpXTH2 in ETH-treated samples reached the maximum level at 12 h. Moreover, the relative expression of both DpXTH1 and DpXTH2 in dahlia flower petals treated with ETH at early stage of flowering was higher than that of the samples treated at other flowering stages; in 1-MCP-treated samples, it was higher at full-flowering stage than at other stages. The maximum relative expression levels of DpXTH1 and DpXTH2 in ETH- and 1-MCP-treated samples were all higher than those of control.[Conclusions] Exogenous ethylene can accelerate the opening of dahlia flowers, and 1-MCP can inhibit this process, so they can be used to regulate development of dahlia flowers.展开更多
In order to understand the mechanism of spathe color variation in Anthurium andraeanum at the protein level,the leaves,inflorescences and spathes of the wild type and two mutants of A.andraeanum‘Madural’were used as...In order to understand the mechanism of spathe color variation in Anthurium andraeanum at the protein level,the leaves,inflorescences and spathes of the wild type and two mutants of A.andraeanum‘Madural’were used as research objects in which the differential expression of proteins related to flower color mutants was analyzed by one-dimensional electrophoresis and mass spectrometry(1-DE/MS).The 1-DE patterns showed that the protein components expressed highly in spathes were mainly concentrated in the molecular weight range of 20-42 kD,and differential bands were detected between the wild type and the mutant,while no significant differences were detected in the leaf and inflorescence proteins.According to the results of mass spectrometry analysis of the differential bands,21 known functional proteins involved in life processes such as glucose metabolism,resistance,cytoskeleton,gene regulation and signal transduction were identified.It showed that in addition to the influences from anthocyanidins,the spathe color variation of A.andraeanum‘Madural’is also regulated by a variety of metabolic pathway-related proteins.展开更多
基金Supported by the Applied Basic Research Program of Suzhou(SNG201605)Kunshan Ecological Agricultural Science and Technology Project(KN1614)
文摘[Objectives]This study was conducted to explore the physiological mechanism of flower color variation in the white mutants of Anthurium andeaeanum.[Methods]The seven white mutants of'Alabama'and'Turenza'were used as materials to analyze the pigment types,flavonoid types and content and anthocyanin content in the wild type and mutants.[Results]The white spathe mainly contained flavonoids,flavonols,dihydroflavonols and dihydroflavonols;the white mutants of'Alabama'had a higher total flavonoid content than the wild type,while the white mutants of'Turenza'showed an opposite trend;and the spathe of the wild type had the highest anthocyanin content,and the pink part of the two-color mutant or the spathe of the binary color mutant contained trace anthocyanins,while no anthocyanins were detected in the white part of the mutants.[Conclusions]The main cause of the white mutants of A.andeaeanum is related to anthocyanin metabolism.
基金Supported by Applied Basic Research Project of Suzhou City(SYN201405)
文摘[Objectives] This study was conducted to investigate the effect of paclobutrazol （PBZ） on heat tolerance of dahlia.[Methods] A dahlia variety Danbanhuang was selected as the experimental material in this study. After 100, 200, 300 or 400 mg/L paclobutrazol was sprayed evenly to the leaves of dahlia seedlings, they were cultured in an incubator at high temperature （35 ℃/30 ℃, day/night） for two days, and then transferred to an incubator at normal temperature （25 ℃/20 ℃, day/night）. The physiological and biochemical parameters of the dahlia seedlings were measured before exposure to high temperature （D0）, after exposure to high temperature for two days （D2） and exposure to normal temperature for one day （R1）.[Results] Compared to the negative control, foliar application of paclobutrazol decreased the content of MDA, increased the contents of chlorophyll, proline, soluble protein, and the activity of SOD, POD and CAT in dahlia plants under heat stress. The contents of MDA, soluble protein and proline in PBZ treated dahlia plants increased when they were subject to high temperature stress （35 ℃/30 ℃, day/night）, and then decreased when the temperature returned to normal （25 ℃/20 ℃, day/night）, and CAT activity decreased at high temperature, and then increased at normal temperature, while the activity of SOD and POD kept rising during the entire experimental period.[Conclusions] Foliar application of paclobutrazol can alleviate the adverse effect caused by high temperature to dahlia plants, and the experimental data provide a theoretical basis for the application of paclobutrazol in dahlia cultivation in South China.
基金Supported by Applied Basic Research Project of Suzhou City(SYN201405)
文摘Objectives] This study was conducted to investigate the regulatory mechanism of ethylene on DpXTH gene expression in dahlia （ Dahlia pinnata Cav.） flower petals.[Methods] Dahlia flower petals were treated with ethephon （ETH） and 1-methylcyclopropene （1-MCP） at different flower development stages and for different durations in vivo . Then, the relative expression of DpXTH1 and DpXTH2 in flower samples in different treatments was quantified with real-time PCR, using β-actin as a reference gene.[Results] The expression of DpXTH1 and DpXTH2 in ETH- or 1-MCP-treated dahlia flower petals increased at first, and decreased subsequently with increasing duration of treatment. The relative expression of both DpXTH1 and DpXTH2 in ETH-treated samples reached the maximum level at 12 h. Moreover, the relative expression of both DpXTH1 and DpXTH2 in dahlia flower petals treated with ETH at early stage of flowering was higher than that of the samples treated at other flowering stages; in 1-MCP-treated samples, it was higher at full-flowering stage than at other stages. The maximum relative expression levels of DpXTH1 and DpXTH2 in ETH- and 1-MCP-treated samples were all higher than those of control.[Conclusions] Exogenous ethylene can accelerate the opening of dahlia flowers, and 1-MCP can inhibit this process, so they can be used to regulate development of dahlia flowers.
基金Supported by the Applied Basic Research Program of Suzhou(SNG201605)Kunshan Ecological Agricultural Science and Technology Project(KN1614)
文摘In order to understand the mechanism of spathe color variation in Anthurium andraeanum at the protein level,the leaves,inflorescences and spathes of the wild type and two mutants of A.andraeanum‘Madural’were used as research objects in which the differential expression of proteins related to flower color mutants was analyzed by one-dimensional electrophoresis and mass spectrometry(1-DE/MS).The 1-DE patterns showed that the protein components expressed highly in spathes were mainly concentrated in the molecular weight range of 20-42 kD,and differential bands were detected between the wild type and the mutant,while no significant differences were detected in the leaf and inflorescence proteins.According to the results of mass spectrometry analysis of the differential bands,21 known functional proteins involved in life processes such as glucose metabolism,resistance,cytoskeleton,gene regulation and signal transduction were identified.It showed that in addition to the influences from anthocyanidins,the spathe color variation of A.andraeanum‘Madural’is also regulated by a variety of metabolic pathway-related proteins.
