The co-chaperone DnaJ plays an important role in protein folding and regulation of various physiological activities, and participates in several pathological processes. DnaJ has been extensively studied in many specie...The co-chaperone DnaJ plays an important role in protein folding and regulation of various physiological activities, and participates in several pathological processes. DnaJ has been extensively studied in many species including humans,drosophila, mushrooms, tomatoes, and Arabidopsis. However, few studies have examined the role of DnaJ in wheat(Triticum aestivum), and the interaction mechanism between TaDnaJs and plant viruses. Here, we identified 236 TaDnaJs and performed a comprehensive genome-wide analysis of conserved domains, gene structure and protein motifs, chromosomal positions and duplication relationships, and cis-acting elements. We grouped these Ta Dna Js according to their domains, and randomly selected six genes from the groups for tissue-specific analysis, and expression profiles analysis under hormone stress, and 17 genes for plant virus infection stress. In qRT-PCR, we found that among the 17 TaDnaJ genes tested, 16 genes were up-regulated after wheat yellow mosaic virus(WYMV) infection, indicating that the TaDnaJ family is involved in plant defense response. Subsequent yeast two-hybrid assays verified the WYMV NIa, NIb and 7 KD proteins interacted with TaDJC(TraesCS7 A02 G506000), which had the most significant changes in gene expression levels after WYMV infection.Insights into the molecular mechanisms of Ta Dna J-mediated stress tolerance and sensitivity could inform different strategies designed to improve crop resistance to abiotic and biotic stress. This study provides a basis for future investigation of the TaDnaJ family and plant defense mechanisms.展开更多
Second—order nonlinear optical(NLO)polymeric matedals have been extensively studied for more than one decade because of their potential applications in integrated optical signal processing⋯.To optimize the comprehens...Second—order nonlinear optical(NLO)polymeric matedals have been extensively studied for more than one decade because of their potential applications in integrated optical signal processing⋯.To optimize the comprehensive properties of this type of materials,several polymer systems,such as vip—host polymers,side.chain polymers,main—chain polymers an d crosslinked polymers combined with NLO chromophores in diferent architecture have been investigated Among them,high chromophore density an d improved orientation stability are obtainable in polymer systems with the majority of chromophores。展开更多
目的比较TruSeq^(®)Exome与NimbleGen SeqCap EZ Human Exome两种外显子捕获试剂在脑瘫患儿中的捕获性能差异,为临床遗传学研究和诊断提供技术选择依据。方法纳入48例散发脑瘫患儿外周血样本,分别采用TruSeq(DNA探针)和NimbleGen(...目的比较TruSeq^(®)Exome与NimbleGen SeqCap EZ Human Exome两种外显子捕获试剂在脑瘫患儿中的捕获性能差异,为临床遗传学研究和诊断提供技术选择依据。方法纳入48例散发脑瘫患儿外周血样本,分别采用TruSeq(DNA探针)和NimbleGen(RNA探针)构建外显子组文库,经Illumina HiSeq 2000平台测序。通过生物信息学分析评估比对率、目标区域覆盖度、变异检出一致性等指标,并基于脑瘫相关基因集(2293个基因)分析捕获性能的临床相关性,采用配对t检验进行统计学分析(显著性阈值α=0.05)。结果NimbleGen和TruSeq两种外显子组捕获试剂盒在基础数据质量(比对率、插入片段长度)和GC含量上差异无统计学意义。然而,在关键性能指标上呈现互补特征,NimbleGen在特定深度覆盖上表现更优(1×覆盖率,P=1.84×10^(-5);20×覆盖率,P=1.49×10^(-20));而TruSeq则展现出更高的Indel检测灵敏度(TruSeq vs.NimbleGen:11371±1689 vs.11274±1670,P=3.24×10^(-7))和罕见变异捕获能力(TruSeq vs.NimbleGen:3164±766 vs.3072±774,P=1.20×10^(-4)),并成功检出所有11个阳性致病变异(包括NimbleGen漏检的2例)。结论TruSeq凭借更优的变异检出率更适合临床诊断场景,而NimbleGen的覆盖稳定性可能有利于研究性项目。展开更多
基金supported by the National Key R&D Program of China(2018YFD0200507,2017YFD-0201701,and 2018YFD0200408)the National Natural Science Foundation of China(31901954)+3 种基金the Natural Science Foundation of Ningbo City,China(2019A610415 and 2019A610410)the National Key Project for Research on Transgenic Biology,China(2016ZX08002-001)the China Modern Agricultural Industry Technology System of MOF and MARA(CARS-03)the K.C.Wong Magna Funding in Ningbo University,China。
文摘The co-chaperone DnaJ plays an important role in protein folding and regulation of various physiological activities, and participates in several pathological processes. DnaJ has been extensively studied in many species including humans,drosophila, mushrooms, tomatoes, and Arabidopsis. However, few studies have examined the role of DnaJ in wheat(Triticum aestivum), and the interaction mechanism between TaDnaJs and plant viruses. Here, we identified 236 TaDnaJs and performed a comprehensive genome-wide analysis of conserved domains, gene structure and protein motifs, chromosomal positions and duplication relationships, and cis-acting elements. We grouped these Ta Dna Js according to their domains, and randomly selected six genes from the groups for tissue-specific analysis, and expression profiles analysis under hormone stress, and 17 genes for plant virus infection stress. In qRT-PCR, we found that among the 17 TaDnaJ genes tested, 16 genes were up-regulated after wheat yellow mosaic virus(WYMV) infection, indicating that the TaDnaJ family is involved in plant defense response. Subsequent yeast two-hybrid assays verified the WYMV NIa, NIb and 7 KD proteins interacted with TaDJC(TraesCS7 A02 G506000), which had the most significant changes in gene expression levels after WYMV infection.Insights into the molecular mechanisms of Ta Dna J-mediated stress tolerance and sensitivity could inform different strategies designed to improve crop resistance to abiotic and biotic stress. This study provides a basis for future investigation of the TaDnaJ family and plant defense mechanisms.
基金the National Science Foundation of China(No.29774018)for Financial Support
文摘Second—order nonlinear optical(NLO)polymeric matedals have been extensively studied for more than one decade because of their potential applications in integrated optical signal processing⋯.To optimize the comprehensive properties of this type of materials,several polymer systems,such as vip—host polymers,side.chain polymers,main—chain polymers an d crosslinked polymers combined with NLO chromophores in diferent architecture have been investigated Among them,high chromophore density an d improved orientation stability are obtainable in polymer systems with the majority of chromophores。
