OBJECTIVE:To observe the analgesic effects of Tuina on neuropathic pain(NPP)and the underlying mechanisms.METHODS:Forty-eight Sprague-Dawley(SD)rats were assigned by random into three treatment groups:sham,chronic con...OBJECTIVE:To observe the analgesic effects of Tuina on neuropathic pain(NPP)and the underlying mechanisms.METHODS:Forty-eight Sprague-Dawley(SD)rats were assigned by random into three treatment groups:sham,chronic constriction injury(CCI),and Tuina.Each group contained sixteen rats.CCI model was generated by ligating the right sciatic nerve.Behavioral changes of CCI were assessed by the paw withdrawal threshold(PWT)and paw withdrawal latency(PWL).In addition,biochemical techniques such as immunofluorescence staining,enzyme-linked immunosorbent assay(ELISA)and Western blotting were used to profile levels of microglia activation and inflammatory factors in the spinal dorsal horn(SDH)of rats.Tuina(clockwise pressing and rubbing)was performed at Chengshan(BL57)to observe the analgesic effects on CCI rats and the underlying mechanisms.RESULTS:Rats with CCI experienced significant reduction in the PWT and PWL of the right hind paw relative to CCI group at day 3.Tuina treatment rescued this situation significantly on days 10 and 14.Besides,Iba-1,microglia M1 receptor CD68,tumor necrosis factor-α(TNF-α),and interleukin-1β(IL-1β)were higher in the right SDH for CCI group compared to the sham group on day 14.As expected,Tuina partially downregulated the CCI-induced overexpressed Iba-1,CD68,TNF-α,and IL-1βin the SDH of CCI model.CONCLUSION:Tuina induces a time-dependent cumulative analgesic effect in CCI rats by inhibiting the activation of microglia and the secretion of IL-1βand TNF-αin SDH.展开更多
目的探讨以针刀为主的综合治疗方案治疗后外侧型颈椎间盘突出症的临床疗效。方法选择2020年1月—2022年1月泉州市正骨医院针灸推拿科收治的82例后外侧型颈椎间盘突出症患者为研究对象,给予针刀结合手法、药物、牵引等综合疗法治疗。记...目的探讨以针刀为主的综合治疗方案治疗后外侧型颈椎间盘突出症的临床疗效。方法选择2020年1月—2022年1月泉州市正骨医院针灸推拿科收治的82例后外侧型颈椎间盘突出症患者为研究对象,给予针刀结合手法、药物、牵引等综合疗法治疗。记录临床疗效、治疗前后的疼痛情况、颈椎功能障碍情况、生活质量、不良反应。结果(1)痊愈20例(24.2%),显效36例(43.9%),有效19例(23.2%),无效7例(8.5%),总体有效率为91.5%。(2)治疗前,患者平均视觉模拟评分法(visual analogue scale,VAS)评分为(5.268±0.969)分;治疗后,VAS评分为(2.317±0.910)分,差异有统计学意义(P<0.05)。(3)治疗前,颈椎功能障碍指数量表(neck disability index,NDI)评分为(434.965±4.182)分;治疗后,NDI评分为(12.122±4.448)分,差异有统计学意义(P<0.05)。(4)治疗前,患者平均生活质量调查问卷(European organization for research and treatment of cancer quality of life questionnaire core30 items,QLQ-C30)评分为(50.622±10.426)分;治疗后,QLQ-C30评分为(82.136±3.915)分,差异有统计学意义(P<0.05)。(5)治疗期间1例患者因针刀对颈椎的刺激而发生血肿,不良反应发生率为1.22%。结论针刀为主综合治疗后外侧型颈椎间盘突出症的临床疗效肯定,能有效缓解疼痛,改善患者颈椎功能障碍,是治疗此疾病的一种较理想的疗法。展开更多
基金National Natural Science Foundation of China:Study on the Mechanism of Tuina Inhibiting the Spinal Cord Central Sensitization of Neuropathic Pain through Regulates Interleukin-10/β-Endorphin Pathway in Spinal Microglia(No.82205302)Study on the Mechanism of Peripheral and Central Pain in Lumbar disc herniation with Nerve Injury and the Analgesia Mechanism of Tuina(No.82030121)+1 种基金Shanghai Sailing Program:Study on the Analgesic Mechanism of Tuina Inhibiting Spinal Cord Central Sensitization based on Microglia and Related Pro-inflammatory Factors(20YF1450900)Science Foundation of Yueyang Hospital of Integrated Traditional Chinese and Western Medicine:Study on the Mechanism of Tuina Analgesia based on Spinal Cord Microglia and Inflammatory Factors(2021yygq03)。
文摘OBJECTIVE:To observe the analgesic effects of Tuina on neuropathic pain(NPP)and the underlying mechanisms.METHODS:Forty-eight Sprague-Dawley(SD)rats were assigned by random into three treatment groups:sham,chronic constriction injury(CCI),and Tuina.Each group contained sixteen rats.CCI model was generated by ligating the right sciatic nerve.Behavioral changes of CCI were assessed by the paw withdrawal threshold(PWT)and paw withdrawal latency(PWL).In addition,biochemical techniques such as immunofluorescence staining,enzyme-linked immunosorbent assay(ELISA)and Western blotting were used to profile levels of microglia activation and inflammatory factors in the spinal dorsal horn(SDH)of rats.Tuina(clockwise pressing and rubbing)was performed at Chengshan(BL57)to observe the analgesic effects on CCI rats and the underlying mechanisms.RESULTS:Rats with CCI experienced significant reduction in the PWT and PWL of the right hind paw relative to CCI group at day 3.Tuina treatment rescued this situation significantly on days 10 and 14.Besides,Iba-1,microglia M1 receptor CD68,tumor necrosis factor-α(TNF-α),and interleukin-1β(IL-1β)were higher in the right SDH for CCI group compared to the sham group on day 14.As expected,Tuina partially downregulated the CCI-induced overexpressed Iba-1,CD68,TNF-α,and IL-1βin the SDH of CCI model.CONCLUSION:Tuina induces a time-dependent cumulative analgesic effect in CCI rats by inhibiting the activation of microglia and the secretion of IL-1βand TNF-αin SDH.
文摘目的探讨以针刀为主的综合治疗方案治疗后外侧型颈椎间盘突出症的临床疗效。方法选择2020年1月—2022年1月泉州市正骨医院针灸推拿科收治的82例后外侧型颈椎间盘突出症患者为研究对象,给予针刀结合手法、药物、牵引等综合疗法治疗。记录临床疗效、治疗前后的疼痛情况、颈椎功能障碍情况、生活质量、不良反应。结果(1)痊愈20例(24.2%),显效36例(43.9%),有效19例(23.2%),无效7例(8.5%),总体有效率为91.5%。(2)治疗前,患者平均视觉模拟评分法(visual analogue scale,VAS)评分为(5.268±0.969)分;治疗后,VAS评分为(2.317±0.910)分,差异有统计学意义(P<0.05)。(3)治疗前,颈椎功能障碍指数量表(neck disability index,NDI)评分为(434.965±4.182)分;治疗后,NDI评分为(12.122±4.448)分,差异有统计学意义(P<0.05)。(4)治疗前,患者平均生活质量调查问卷(European organization for research and treatment of cancer quality of life questionnaire core30 items,QLQ-C30)评分为(50.622±10.426)分;治疗后,QLQ-C30评分为(82.136±3.915)分,差异有统计学意义(P<0.05)。(5)治疗期间1例患者因针刀对颈椎的刺激而发生血肿,不良反应发生率为1.22%。结论针刀为主综合治疗后外侧型颈椎间盘突出症的临床疗效肯定,能有效缓解疼痛,改善患者颈椎功能障碍,是治疗此疾病的一种较理想的疗法。
