The Fringe Projection Profilometry(FPP)system with a single exposure time or a single projection intensity is limited by the dynamic range of the camera,which can lead to overexposure and underexposure of the image,re...The Fringe Projection Profilometry(FPP)system with a single exposure time or a single projection intensity is limited by the dynamic range of the camera,which can lead to overexposure and underexposure of the image,resulting in point cloud loss or reduced accuracy.To address this issue,unlike the pixel modulation method of projectors,we utilize the characteristics of color projectors where the intensity of the three-channel LED can be controlled independently.We propose a method for separating the projector's three-channel light intensity,combined with a color camera,to achieve single exposure and multi-intensity image acquisition.Further,the crosstalk coefficient is applied to predict the three-channel reflectance of the measured object.By integrating clustering and channel mapping,we establish a pixel-level mapping model between the projector's three-channel current and the camera's three-channel image intensity,which realizes the optimal projection current prediction and the high dynamic range(HDR)image acquisition.The proposed method allows for high-precision three-dimensional(3D)data acquisition of HDR scenes with a single exposure.The effectiveness of this method has been validated through experiments with standard planes and standard steps,showing a significant reduction in mean absolute error(44.6%)compared to existing singleexposure HDR methods.Additionally,the number of images required for acquisition is significantly reduced(by 70.8%)compared to multi-exposure fusion methods.This proposed method has great potential in various FPP-related fields.展开更多
A systematic understanding of the mechanism in the rectification and capacitance of nanochannels and their regulation with the electrolyte concentration and electrical bias is pivotal for its wide applications to nano...A systematic understanding of the mechanism in the rectification and capacitance of nanochannels and their regulation with the electrolyte concentration and electrical bias is pivotal for its wide applications to nanofluidic electronics,ion separation,energy storage,and molecule sensing.Single unipolar and bipolar cylindrical nanochannels through polymer film were fabricated using single ion bombardment and track etching.Cyclic voltammetry results show that the bipolar nanochannel switches from rectification to capacitance as the electrolyte concentration decreases.Electrochemical impedance spectroscopy revealed that the capacitive impedance fraction in the bipolar nanochannel is regulated by electrolyte concentration and voltage.The switch from rectification to capacitance in the polymer nanochannel is well explained through a fluidic p-n junction model with a variable ion depletion layer regulated by the applied bias voltage,which is supported by the multi-physics simulation using Poisson-Nernst-Planck and Navier-Stokes solution.This work provides a mechanistic insight into the ionic current rectification and ionic capacitance in complex ionic nanochannels and paves the way for biomimetic nanofluidic electronics design.展开更多
Objective:Patient-derived xenograft(PDX)models have shown great promise in preclinical and translational applications,but their consistency with primary tumors in phenotypic,genetic,and pharmacodynamic heterogeneity h...Objective:Patient-derived xenograft(PDX)models have shown great promise in preclinical and translational applications,but their consistency with primary tumors in phenotypic,genetic,and pharmacodynamic heterogeneity has not been well-studied.This study aimed to establish a PDX repository for non-small cell lung cancer(NSCLC)and to further elucidate whether it could preserve the heterogeneity within and between tumors in patients.Methods:A total of 75 surgically resected NSCLC specimens were implanted into immunodeficient NOD/SCID mice.Based on the successful establishment of the NSCLC PDX model,we compared the expressions of vimentin,Ki67,EGFR,and PD-L1 proteins between cancer tissues and PDX models using hematoxylin and eosin staining and immunohistochemical staining.In addition,we detected whole gene expression profiling between primary tumors and PDX generations.We also performed whole exome sequencing(WES)analysis in 17 first generation xenografts to further assess whether PDXs retained the patient heterogeneities.Finally,paclitaxel,cisplatin,doxorubicin,atezolizumab,afatininb,and AZD4547 were used to evaluate the responses of PDX models to the standard-of-care agents.Results:A large collection of serially transplantable PDX models for NSCLC were successfully developed.The histology and pathological immunohistochemistry of PDX xenografts were consistent with the patients’tumor samples.WES and RNA-seq further confirmed that PDX accurately replicated the molecular heterogeneities of primary tumors.Similar to clinical patients,PDX models responded differentially to the standard-of-care treatment,including chemo-,targeted-and immuno-therapeutics.Conclusions:Our established PDX models of NSCLC faithfully reproduced the molecular,histopathological,and therapeutic characteristics,as well as the corresponding tumor heterogeneities,which provides a clinically relevant platform for drug screening,biomarker discovery,and translational research.展开更多
文摘The Fringe Projection Profilometry(FPP)system with a single exposure time or a single projection intensity is limited by the dynamic range of the camera,which can lead to overexposure and underexposure of the image,resulting in point cloud loss or reduced accuracy.To address this issue,unlike the pixel modulation method of projectors,we utilize the characteristics of color projectors where the intensity of the three-channel LED can be controlled independently.We propose a method for separating the projector's three-channel light intensity,combined with a color camera,to achieve single exposure and multi-intensity image acquisition.Further,the crosstalk coefficient is applied to predict the three-channel reflectance of the measured object.By integrating clustering and channel mapping,we establish a pixel-level mapping model between the projector's three-channel current and the camera's three-channel image intensity,which realizes the optimal projection current prediction and the high dynamic range(HDR)image acquisition.The proposed method allows for high-precision three-dimensional(3D)data acquisition of HDR scenes with a single exposure.The effectiveness of this method has been validated through experiments with standard planes and standard steps,showing a significant reduction in mean absolute error(44.6%)compared to existing singleexposure HDR methods.Additionally,the number of images required for acquisition is significantly reduced(by 70.8%)compared to multi-exposure fusion methods.This proposed method has great potential in various FPP-related fields.
基金supported by the National Key R&D Program of China(Grant No.2021YFA1601400)the National Natural Science Foundation of China(Grant Nos.12241201,1197283,12375287,and U1632271).
文摘A systematic understanding of the mechanism in the rectification and capacitance of nanochannels and their regulation with the electrolyte concentration and electrical bias is pivotal for its wide applications to nanofluidic electronics,ion separation,energy storage,and molecule sensing.Single unipolar and bipolar cylindrical nanochannels through polymer film were fabricated using single ion bombardment and track etching.Cyclic voltammetry results show that the bipolar nanochannel switches from rectification to capacitance as the electrolyte concentration decreases.Electrochemical impedance spectroscopy revealed that the capacitive impedance fraction in the bipolar nanochannel is regulated by electrolyte concentration and voltage.The switch from rectification to capacitance in the polymer nanochannel is well explained through a fluidic p-n junction model with a variable ion depletion layer regulated by the applied bias voltage,which is supported by the multi-physics simulation using Poisson-Nernst-Planck and Navier-Stokes solution.This work provides a mechanistic insight into the ionic current rectification and ionic capacitance in complex ionic nanochannels and paves the way for biomimetic nanofluidic electronics design.
基金supported by the National Natural Science Foundation of China(Grant Nos.81101143,81572617,and 81630101)the Sichuan Province Science and Technology Support Program(Grant Nos.2019JDRC0019 and 2018SZ0009)+2 种基金1.3.5 project for disciplines of excellence,West China Hospital,Sichuan University(Grant No.ZYJC18026)The Science and Technology Project of the Health Planning Committee of Sichuan(Grant No.19PJ242)Chengdu science and technology Support Program(Grant No.2019-YFYF-00090-SN)。
文摘Objective:Patient-derived xenograft(PDX)models have shown great promise in preclinical and translational applications,but their consistency with primary tumors in phenotypic,genetic,and pharmacodynamic heterogeneity has not been well-studied.This study aimed to establish a PDX repository for non-small cell lung cancer(NSCLC)and to further elucidate whether it could preserve the heterogeneity within and between tumors in patients.Methods:A total of 75 surgically resected NSCLC specimens were implanted into immunodeficient NOD/SCID mice.Based on the successful establishment of the NSCLC PDX model,we compared the expressions of vimentin,Ki67,EGFR,and PD-L1 proteins between cancer tissues and PDX models using hematoxylin and eosin staining and immunohistochemical staining.In addition,we detected whole gene expression profiling between primary tumors and PDX generations.We also performed whole exome sequencing(WES)analysis in 17 first generation xenografts to further assess whether PDXs retained the patient heterogeneities.Finally,paclitaxel,cisplatin,doxorubicin,atezolizumab,afatininb,and AZD4547 were used to evaluate the responses of PDX models to the standard-of-care agents.Results:A large collection of serially transplantable PDX models for NSCLC were successfully developed.The histology and pathological immunohistochemistry of PDX xenografts were consistent with the patients’tumor samples.WES and RNA-seq further confirmed that PDX accurately replicated the molecular heterogeneities of primary tumors.Similar to clinical patients,PDX models responded differentially to the standard-of-care treatment,including chemo-,targeted-and immuno-therapeutics.Conclusions:Our established PDX models of NSCLC faithfully reproduced the molecular,histopathological,and therapeutic characteristics,as well as the corresponding tumor heterogeneities,which provides a clinically relevant platform for drug screening,biomarker discovery,and translational research.
