Sika deer are known to prefer oak leaves,which are rich in tannins and toxic to most mammals;however,the genetic mechanisms underlying their unique ability to adapt to living in the jungle are still unclear.In identif...Sika deer are known to prefer oak leaves,which are rich in tannins and toxic to most mammals;however,the genetic mechanisms underlying their unique ability to adapt to living in the jungle are still unclear.In identifying the mechanism responsible for the tolerance of a highly toxic diet,we have made a major advancement by explaining the genome of sika deer.We generated the first high-quality,chromosome-level genome assembly of sika deer and measured the correlation between tannin intake and RNA expression in 15 tissues through 180 experiments.Comparative genome analyses showed that the UGT and CYP gene families are functionally involved in the adaptation of sika deer to high-tannin food,especially the expansion of the UGT family 2 subfamily B of UGT genes.The first chromosome-level assembly and genetic characterization of the tolerance to a highly toxic diet suggest that the sika deer genome may serve as an essential resource for understanding evolutionary events and tannin adaptation.Our study provides a paradigm of comparative expressive genomics that can be applied to the study of unique biological features in non-model animals.展开更多
文摘目的观察运动疗法配合肌效贴治疗膝骨关节炎(knee osteoarthritis,KOA)的疗效。方法选取2019年1月至2021年12月就诊于阿克苏地区第一人民医院康复科的61例KOA患者作为研究对象,随机分为试验组30例、对照组31例。对照组采用针灸推拿治疗,试验组在对照组的基础上采用运动疗法配合肌效贴治疗,治疗2个疗程,比较两组患者治疗前、治疗1周后、治疗2周后的临床症状评分、视觉模拟量表(visual analogue scale,VAS)评分、西安大略麦克马斯特大学骨关节炎指数(Western Ontario and McMaster University osteoarthritis index,WOMAC)评分、Lysholm膝关节评分。结果两组患者治疗后的临床症状评分、VAS评分、WOMAC评分、Lysholm评分比较,差异有统计学意义(P<0.05);且随着治疗时间的延长,试验组患者的临床症状评分、VAS评分、WOMAC评分降低幅度大于对照组,Lysholm评分升高幅度大于对照组(P<0.05);试验组的总有效率为90.00%,对照组的总有效率74.19%,两组比较差异有统计学意义(P<0.05)。结论运动疗法结合肌效贴治疗膝骨关节炎可显著降低临床症状评分、VAS评分、WOMAC评分,增加Lysholm评分,在改善患者的临床症状、膝关节功能和疼痛方面优于针灸推拿治疗。
基金This work was supported by the National Key R&D Program of China(Grant No.2018YFD0502204)the Agricultural Science and Technology Innovation Program of China(Grant No.CAAS-ASTIP-2019-ISAPS)+1 种基金the Special Animal Genetic Resources Platform of National Scientific and Technical Infrastructure Center(Grant No.NSTIC TZDWZYK2019)the Sika deer Genome Project of China(Grant No.20140309016YY).
文摘Sika deer are known to prefer oak leaves,which are rich in tannins and toxic to most mammals;however,the genetic mechanisms underlying their unique ability to adapt to living in the jungle are still unclear.In identifying the mechanism responsible for the tolerance of a highly toxic diet,we have made a major advancement by explaining the genome of sika deer.We generated the first high-quality,chromosome-level genome assembly of sika deer and measured the correlation between tannin intake and RNA expression in 15 tissues through 180 experiments.Comparative genome analyses showed that the UGT and CYP gene families are functionally involved in the adaptation of sika deer to high-tannin food,especially the expansion of the UGT family 2 subfamily B of UGT genes.The first chromosome-level assembly and genetic characterization of the tolerance to a highly toxic diet suggest that the sika deer genome may serve as an essential resource for understanding evolutionary events and tannin adaptation.Our study provides a paradigm of comparative expressive genomics that can be applied to the study of unique biological features in non-model animals.
