目的探讨生长阻滞和DNA损伤诱导蛋白45(growth arrest and DNA damage induced protein 45,Gadd45)A和Gadd45G在前列腺癌(prostate cancer,PCa)组织中的表达及其与临床病理特征和预后的关系。方法选取2020年1月~2022年1月期间沧州市人...目的探讨生长阻滞和DNA损伤诱导蛋白45(growth arrest and DNA damage induced protein 45,Gadd45)A和Gadd45G在前列腺癌(prostate cancer,PCa)组织中的表达及其与临床病理特征和预后的关系。方法选取2020年1月~2022年1月期间沧州市人民医院收治的80例PCa患者的癌组织作为PCa组,癌旁组织作为对照组,行前列腺根治术后随访3年,并根据术后复发情况将其分为复发组(n=31)和未复发组(n=49)。收集PCa患者临床病理特征资料;采用蛋白质免疫印迹法检测两组Gadd45A、Gadd45G表达情况,并分析其表达水平与患者临床病理特征的关系;绘制受试者工作特征(receiver operating characteristic,ROC)曲线评估Gadd45A、Gadd45G对PCa患者术后复发预测价值;Cox回归分析影响PCa患者术后复发的风险因素。结果PCa组Gadd45A和Gadd45G表达水平均明显低于对照组(0.79±0.16 vs.1.00±0.26,0.60±0.14 vs.0.98±0.23,P<0.05),且其低表达与PSA水平升高、TNM分期进展、淋巴结转移显著相关(χ2=10.570、9.642、9.657,P<0.05);复发PCa患者Gadd45A、Gadd45G表达水平明显低于未复发PCa患者(0.64±0.11 vs.0.89±0.20,0.47±0.10 vs.0.68±0.16,P<0.05),且Gadd45A、Gadd45G单独及联合预测PCa患者术后复发的曲线下面积(area under the curve,AUC)分别为0.824、0.813、0.922,联合优于单独预测(Z二者联合-Gadd45A=2.384、P=0.017,Z二者联合-Gadd45G=2.777,P=0.006);TNM分期[风险比(hazard ratio,HR)=2.038,95%可信区间(confidence interval,CI)1.161~3.577]、淋巴结转移(HR=1.802,95%CI1.093~2.970)、PSA水平(HR=1.736,95%CI 1.047~2.878)及Gadd45A(HR=0.925,95%CI 0.872~0.981)和Gadd45G(HR=0.766,95%CI 0.610~0.962)是PCa患者术后复发的影响因素(P<0.05)。结论在PCa患者组织中,Gadd45A和Gadd45G表达水平降低,其表达水平与PSA水平、TNM分期、淋巴结转移有关,且二者对PCa患者术后复发的预测效能较高,具有一定的临床价值。展开更多
A green,renewable composite was designed and fabricated based on self-assembly of cyclodextrin metal-organic framework(CD-MOF)on graphene oxide(GO).Then,the GO@CD-MOF was embedded in 0.45μm PTFE membrane to produce a...A green,renewable composite was designed and fabricated based on self-assembly of cyclodextrin metal-organic framework(CD-MOF)on graphene oxide(GO).Then,the GO@CD-MOF was embedded in 0.45μm PTFE membrane to produce a dual-functional membrane which could carry out sample enrichment by capturing naringin molecules.The membrane filter was further improved by investigating the effects of the experimental parameters including amount of GO@CD-MOF,enrichment time and elution solvent on enrichment efficiency of naringin.Further,the present method had been successfully applied to citrus sample and obtained satisfied recovery value(79.7%-100.3%).Moreover,the extraction of naringin can be achieved for 2 min,and GO@CD-MOF loaded membrane can be reused at least for 5 times.The results demonstrate that the fabrication of the novel filter membrane based on GO@CD-MOF is a fast,simple and reliable,and possesses great potential in the determination of naringin from real samples by dual-function of separation and enrichment.展开更多
Phosphoenolpyruvate carboxylase（PEPC;EC 4.1.1.31） catalyses phosphoenolpyruvate（PEP） to yield oxaloacetate,which is involved in protein biosynthesis.Pyruvate kinase（PK;EC 2.7.1.40） catalyzes PEP to yield pyruvat...Phosphoenolpyruvate carboxylase（PEPC;EC 4.1.1.31） catalyses phosphoenolpyruvate（PEP） to yield oxaloacetate,which is involved in protein biosynthesis.Pyruvate kinase（PK;EC 2.7.1.40） catalyzes PEP to yield pyruvate,which is involved in fatty acid synthesis.In this study,five PEPC genes（AhPEPC1,AhPEPC2,AhPEPC3,AhPEPC4,and AhPEPC5） from peanut have been cloned.Using a quantitative real-time RT-PCR approach,the expression pattern of each gene was monitored during the seed development of four peanut varieties（E11,Hebeigaoyou,Naihan 1,and Huayu 26）.It was found that these five genes shared similar expression behaviors over the developmental stages of E11 with high expression levels at 30 and 40 d after pegging（DAP）;whereas these five genes showed irregular expression patterns during the seed development of Hebeigaoyou.In Naihan 1 and Huayu 26,the expression levels of the five genes remained relatively high in the first stage.The PEPC activity was monitored during the seed development of four peanut varieties and seed oil content was also characterized during whole period of seed development.The PEPC activity followed the oil accumulation pattern during the early stages of development but they showed a significantly negative correlation thereafter.These results suggested that PEPC may play an important role in lipid accumulation during the seed development of four peanut varieties tested.展开更多
Objective To investigate the mechanisms underlying ozone-induced inactivation of poliovirus type 1(PV1). Methods We used cell culture, long‐overlapping RT‐PCR, and spot hybridization assays to verify and accurately ...Objective To investigate the mechanisms underlying ozone-induced inactivation of poliovirus type 1(PV1). Methods We used cell culture, long‐overlapping RT‐PCR, and spot hybridization assays to verify and accurately locate the sites of action of ozone that cause PV1 inactivation. We also employed recombinant viral genome RNA infection models to confirm our observations. Results Our results indicated that ozone inactivated PV1 primarily by disrupting the 5′-non‐coding region(5′‐NCR) of the PV1 genome. Further study revealed that ozone specifically damaged the 80‐124 nucleotide(nt) region in the 5′-NCR. Recombinant viral genome RNA infection models confirmed that PV1 lacking this region was non‐infectious. Conclusion In this study, we not only elucidated the mechanisms by which ozone induces PV1 inactivation but also determined that the 80‐124 nt region in the 5′‐NCR is targeted by ozone to achieve this inactivation.展开更多
文摘目的探讨生长阻滞和DNA损伤诱导蛋白45(growth arrest and DNA damage induced protein 45,Gadd45)A和Gadd45G在前列腺癌(prostate cancer,PCa)组织中的表达及其与临床病理特征和预后的关系。方法选取2020年1月~2022年1月期间沧州市人民医院收治的80例PCa患者的癌组织作为PCa组,癌旁组织作为对照组,行前列腺根治术后随访3年,并根据术后复发情况将其分为复发组(n=31)和未复发组(n=49)。收集PCa患者临床病理特征资料;采用蛋白质免疫印迹法检测两组Gadd45A、Gadd45G表达情况,并分析其表达水平与患者临床病理特征的关系;绘制受试者工作特征(receiver operating characteristic,ROC)曲线评估Gadd45A、Gadd45G对PCa患者术后复发预测价值;Cox回归分析影响PCa患者术后复发的风险因素。结果PCa组Gadd45A和Gadd45G表达水平均明显低于对照组(0.79±0.16 vs.1.00±0.26,0.60±0.14 vs.0.98±0.23,P<0.05),且其低表达与PSA水平升高、TNM分期进展、淋巴结转移显著相关(χ2=10.570、9.642、9.657,P<0.05);复发PCa患者Gadd45A、Gadd45G表达水平明显低于未复发PCa患者(0.64±0.11 vs.0.89±0.20,0.47±0.10 vs.0.68±0.16,P<0.05),且Gadd45A、Gadd45G单独及联合预测PCa患者术后复发的曲线下面积(area under the curve,AUC)分别为0.824、0.813、0.922,联合优于单独预测(Z二者联合-Gadd45A=2.384、P=0.017,Z二者联合-Gadd45G=2.777,P=0.006);TNM分期[风险比(hazard ratio,HR)=2.038,95%可信区间(confidence interval,CI)1.161~3.577]、淋巴结转移(HR=1.802,95%CI1.093~2.970)、PSA水平(HR=1.736,95%CI 1.047~2.878)及Gadd45A(HR=0.925,95%CI 0.872~0.981)和Gadd45G(HR=0.766,95%CI 0.610~0.962)是PCa患者术后复发的影响因素(P<0.05)。结论在PCa患者组织中,Gadd45A和Gadd45G表达水平降低,其表达水平与PSA水平、TNM分期、淋巴结转移有关,且二者对PCa患者术后复发的预测效能较高,具有一定的临床价值。
基金the Project of Ningxia Key R&D Plan(Nos.2022BEG03070,2020BEB04009,2019BFH02014)Ningxia Scientific and Technological Innovation Team of Industrial Water Treatment and RecyclingNational Natural Science Foundation of China(No.22006079)。
文摘A green,renewable composite was designed and fabricated based on self-assembly of cyclodextrin metal-organic framework(CD-MOF)on graphene oxide(GO).Then,the GO@CD-MOF was embedded in 0.45μm PTFE membrane to produce a dual-functional membrane which could carry out sample enrichment by capturing naringin molecules.The membrane filter was further improved by investigating the effects of the experimental parameters including amount of GO@CD-MOF,enrichment time and elution solvent on enrichment efficiency of naringin.Further,the present method had been successfully applied to citrus sample and obtained satisfied recovery value(79.7%-100.3%).Moreover,the extraction of naringin can be achieved for 2 min,and GO@CD-MOF loaded membrane can be reused at least for 5 times.The results demonstrate that the fabrication of the novel filter membrane based on GO@CD-MOF is a fast,simple and reliable,and possesses great potential in the determination of naringin from real samples by dual-function of separation and enrichment.
基金supported by the China Agriculture Research System (CARS-14)the National Natural Science Foundation of China (31000728,31100205)+2 种基金the Natural Science Fundation of Shangdong Province,China(ZR2009DQ004,ZR2011CQ036)the Promotive Research Fund for Young and Middle-Aged Scientisits of Shandong Province,China (BS2010NY023)the Qingdao Municipal Science and Technology Plan Project,China (11-2-4-9-(3)-jch,11-2-3-26-nsh)
文摘Phosphoenolpyruvate carboxylase（PEPC;EC 4.1.1.31） catalyses phosphoenolpyruvate（PEP） to yield oxaloacetate,which is involved in protein biosynthesis.Pyruvate kinase（PK;EC 2.7.1.40） catalyzes PEP to yield pyruvate,which is involved in fatty acid synthesis.In this study,five PEPC genes（AhPEPC1,AhPEPC2,AhPEPC3,AhPEPC4,and AhPEPC5） from peanut have been cloned.Using a quantitative real-time RT-PCR approach,the expression pattern of each gene was monitored during the seed development of four peanut varieties（E11,Hebeigaoyou,Naihan 1,and Huayu 26）.It was found that these five genes shared similar expression behaviors over the developmental stages of E11 with high expression levels at 30 and 40 d after pegging（DAP）;whereas these five genes showed irregular expression patterns during the seed development of Hebeigaoyou.In Naihan 1 and Huayu 26,the expression levels of the five genes remained relatively high in the first stage.The PEPC activity was monitored during the seed development of four peanut varieties and seed oil content was also characterized during whole period of seed development.The PEPC activity followed the oil accumulation pattern during the early stages of development but they showed a significantly negative correlation thereafter.These results suggested that PEPC may play an important role in lipid accumulation during the seed development of four peanut varieties tested.
基金supported by the National Key R&D Program of China [2017YFC1601200,2018YFC1602500]Tianjin Science and Technology Support Program [16YFZCSF00340]
文摘Objective To investigate the mechanisms underlying ozone-induced inactivation of poliovirus type 1(PV1). Methods We used cell culture, long‐overlapping RT‐PCR, and spot hybridization assays to verify and accurately locate the sites of action of ozone that cause PV1 inactivation. We also employed recombinant viral genome RNA infection models to confirm our observations. Results Our results indicated that ozone inactivated PV1 primarily by disrupting the 5′-non‐coding region(5′‐NCR) of the PV1 genome. Further study revealed that ozone specifically damaged the 80‐124 nucleotide(nt) region in the 5′-NCR. Recombinant viral genome RNA infection models confirmed that PV1 lacking this region was non‐infectious. Conclusion In this study, we not only elucidated the mechanisms by which ozone induces PV1 inactivation but also determined that the 80‐124 nt region in the 5′‐NCR is targeted by ozone to achieve this inactivation.
