目的探讨生长阻滞和DNA损伤诱导蛋白45(growth arrest and DNA damage induced protein 45,Gadd45)A和Gadd45G在前列腺癌(prostate cancer,PCa)组织中的表达及其与临床病理特征和预后的关系。方法选取2020年1月~2022年1月期间沧州市人...目的探讨生长阻滞和DNA损伤诱导蛋白45(growth arrest and DNA damage induced protein 45,Gadd45)A和Gadd45G在前列腺癌(prostate cancer,PCa)组织中的表达及其与临床病理特征和预后的关系。方法选取2020年1月~2022年1月期间沧州市人民医院收治的80例PCa患者的癌组织作为PCa组,癌旁组织作为对照组,行前列腺根治术后随访3年,并根据术后复发情况将其分为复发组(n=31)和未复发组(n=49)。收集PCa患者临床病理特征资料;采用蛋白质免疫印迹法检测两组Gadd45A、Gadd45G表达情况,并分析其表达水平与患者临床病理特征的关系;绘制受试者工作特征(receiver operating characteristic,ROC)曲线评估Gadd45A、Gadd45G对PCa患者术后复发预测价值;Cox回归分析影响PCa患者术后复发的风险因素。结果PCa组Gadd45A和Gadd45G表达水平均明显低于对照组(0.79±0.16 vs.1.00±0.26,0.60±0.14 vs.0.98±0.23,P<0.05),且其低表达与PSA水平升高、TNM分期进展、淋巴结转移显著相关(χ2=10.570、9.642、9.657,P<0.05);复发PCa患者Gadd45A、Gadd45G表达水平明显低于未复发PCa患者(0.64±0.11 vs.0.89±0.20,0.47±0.10 vs.0.68±0.16,P<0.05),且Gadd45A、Gadd45G单独及联合预测PCa患者术后复发的曲线下面积(area under the curve,AUC)分别为0.824、0.813、0.922,联合优于单独预测(Z二者联合-Gadd45A=2.384、P=0.017,Z二者联合-Gadd45G=2.777,P=0.006);TNM分期[风险比(hazard ratio,HR)=2.038,95%可信区间(confidence interval,CI)1.161~3.577]、淋巴结转移(HR=1.802,95%CI1.093~2.970)、PSA水平(HR=1.736,95%CI 1.047~2.878)及Gadd45A(HR=0.925,95%CI 0.872~0.981)和Gadd45G(HR=0.766,95%CI 0.610~0.962)是PCa患者术后复发的影响因素(P<0.05)。结论在PCa患者组织中,Gadd45A和Gadd45G表达水平降低,其表达水平与PSA水平、TNM分期、淋巴结转移有关,且二者对PCa患者术后复发的预测效能较高,具有一定的临床价值。展开更多
A green,renewable composite was designed and fabricated based on self-assembly of cyclodextrin metal-organic framework(CD-MOF)on graphene oxide(GO).Then,the GO@CD-MOF was embedded in 0.45μm PTFE membrane to produce a...A green,renewable composite was designed and fabricated based on self-assembly of cyclodextrin metal-organic framework(CD-MOF)on graphene oxide(GO).Then,the GO@CD-MOF was embedded in 0.45μm PTFE membrane to produce a dual-functional membrane which could carry out sample enrichment by capturing naringin molecules.The membrane filter was further improved by investigating the effects of the experimental parameters including amount of GO@CD-MOF,enrichment time and elution solvent on enrichment efficiency of naringin.Further,the present method had been successfully applied to citrus sample and obtained satisfied recovery value(79.7%-100.3%).Moreover,the extraction of naringin can be achieved for 2 min,and GO@CD-MOF loaded membrane can be reused at least for 5 times.The results demonstrate that the fabrication of the novel filter membrane based on GO@CD-MOF is a fast,simple and reliable,and possesses great potential in the determination of naringin from real samples by dual-function of separation and enrichment.展开更多
文摘目的探讨生长阻滞和DNA损伤诱导蛋白45(growth arrest and DNA damage induced protein 45,Gadd45)A和Gadd45G在前列腺癌(prostate cancer,PCa)组织中的表达及其与临床病理特征和预后的关系。方法选取2020年1月~2022年1月期间沧州市人民医院收治的80例PCa患者的癌组织作为PCa组,癌旁组织作为对照组,行前列腺根治术后随访3年,并根据术后复发情况将其分为复发组(n=31)和未复发组(n=49)。收集PCa患者临床病理特征资料;采用蛋白质免疫印迹法检测两组Gadd45A、Gadd45G表达情况,并分析其表达水平与患者临床病理特征的关系;绘制受试者工作特征(receiver operating characteristic,ROC)曲线评估Gadd45A、Gadd45G对PCa患者术后复发预测价值;Cox回归分析影响PCa患者术后复发的风险因素。结果PCa组Gadd45A和Gadd45G表达水平均明显低于对照组(0.79±0.16 vs.1.00±0.26,0.60±0.14 vs.0.98±0.23,P<0.05),且其低表达与PSA水平升高、TNM分期进展、淋巴结转移显著相关(χ2=10.570、9.642、9.657,P<0.05);复发PCa患者Gadd45A、Gadd45G表达水平明显低于未复发PCa患者(0.64±0.11 vs.0.89±0.20,0.47±0.10 vs.0.68±0.16,P<0.05),且Gadd45A、Gadd45G单独及联合预测PCa患者术后复发的曲线下面积(area under the curve,AUC)分别为0.824、0.813、0.922,联合优于单独预测(Z二者联合-Gadd45A=2.384、P=0.017,Z二者联合-Gadd45G=2.777,P=0.006);TNM分期[风险比(hazard ratio,HR)=2.038,95%可信区间(confidence interval,CI)1.161~3.577]、淋巴结转移(HR=1.802,95%CI1.093~2.970)、PSA水平(HR=1.736,95%CI 1.047~2.878)及Gadd45A(HR=0.925,95%CI 0.872~0.981)和Gadd45G(HR=0.766,95%CI 0.610~0.962)是PCa患者术后复发的影响因素(P<0.05)。结论在PCa患者组织中,Gadd45A和Gadd45G表达水平降低,其表达水平与PSA水平、TNM分期、淋巴结转移有关,且二者对PCa患者术后复发的预测效能较高,具有一定的临床价值。
基金the Project of Ningxia Key R&D Plan(Nos.2022BEG03070,2020BEB04009,2019BFH02014)Ningxia Scientific and Technological Innovation Team of Industrial Water Treatment and RecyclingNational Natural Science Foundation of China(No.22006079)。
文摘A green,renewable composite was designed and fabricated based on self-assembly of cyclodextrin metal-organic framework(CD-MOF)on graphene oxide(GO).Then,the GO@CD-MOF was embedded in 0.45μm PTFE membrane to produce a dual-functional membrane which could carry out sample enrichment by capturing naringin molecules.The membrane filter was further improved by investigating the effects of the experimental parameters including amount of GO@CD-MOF,enrichment time and elution solvent on enrichment efficiency of naringin.Further,the present method had been successfully applied to citrus sample and obtained satisfied recovery value(79.7%-100.3%).Moreover,the extraction of naringin can be achieved for 2 min,and GO@CD-MOF loaded membrane can be reused at least for 5 times.The results demonstrate that the fabrication of the novel filter membrane based on GO@CD-MOF is a fast,simple and reliable,and possesses great potential in the determination of naringin from real samples by dual-function of separation and enrichment.
