The influence of fly ash on the fresh properties, mechanical properties and carbonation properties were studied in this paper. The performance of a kind of curing agent which was applied to the hardened concrete surfa...The influence of fly ash on the fresh properties, mechanical properties and carbonation properties were studied in this paper. The performance of a kind of curing agent which was applied to the hardened concrete surface was evaluated. Incorporating large volume of fly ash will risk the concrete carbonation. The curing agent could prevent the concrete carbonation, and the mechanism was explained.展开更多
Taizhou Yangtze River Highway Bridge is a large span suspension bridge with three pylons. The elastic cables are installed to connect the steel tower and the steel box girder. The constraints can increase the safety c...Taizhou Yangtze River Highway Bridge is a large span suspension bridge with three pylons. The elastic cables are installed to connect the steel tower and the steel box girder. The constraints can increase the safety coefficient of the middle saddle, and improve the stress conditions of the middle pylon and decrease the deflection in the middle of the main girder, as well as the longitudinal displacement of the main girder caused by live loads. The anchorage boxes of the elastic cable are installed in the wind fairing outside the vertical web plate of the box girder. Two anchor boxes form a pair and are arranged parallelly. Eight anchor boxes are installed in the bridge. In this paper, the design scheme and the technical difficulties in manufacturing are briefly discussed with the precision control techniques.展开更多
OBJECTIVE To research the effect of naringenin(NAR) on LPS-induced dopaminergic neurons damage and its potential mechanism.METHODS Rats were randomly divided into the following six groups(n=10):control(0.9% NaCl),NAR ...OBJECTIVE To research the effect of naringenin(NAR) on LPS-induced dopaminergic neurons damage and its potential mechanism.METHODS Rats were randomly divided into the following six groups(n=10):control(0.9% NaCl),NAR alone(100 mg·kg-1),LPS(5 μg),LPS+NAR(50 mg·kg-1) and LPS+NAR(100 mg·kg-1).Rats were received a single LPS unilateral injection into the SN pars compacts,after seven daily intragastric administration of NAR,rats′ behavior was analyzed by rotarod test.Then,the expression of TH,IBA-1 and NLRP3 inflammasome were analyzed by Western blotting and immunofluorescence.In vitro experiments,BV-2 cel s were treated with different doses of NAR,and 1 h later,LPS(1 g·L^(-1)) was added to the medium for 24 h,then collect the culture medium and protein for later experiments.The production of IL-1β and IL-18 in culture medium were tested by ELISA,and the production of NO was detected by Griess reagent.The expression of IBA-1,NLRP3 and p-caspase 1 were detected by Western blotting.MN9 D cells were co-cultured with BV2 cells to mimic the animal experiments.MTT assay was used to analyzed the viability of MN9 D cells,and the expression of TH was detected by Western blotting.RESULTS NAR(100 mg · kg-1) could significantly improve the time of rats on the rotating(116.73 s vs 185.45 s,P<0.05).The result of the pathological analysis also suggested that NAR could decrease the activation of microglia as well as the expression of NLRP3 Inflammasome.In addition,NAR also could suppress the expression of pro-inflammatory factor levels,such as IL-1β(P<0.05),IL-18(P<0.05),and the protection of NAR could be inhibited by siR NA NLRP3.Moreover,an in vitro co-culture system with BV2 and MN9 D cells wasused to find the protection of NAR must via microglia,while there is no effect of NAR were directly added to MN9 D cells.CONCLUSION NAR protection of LPS-induced dopaminergic neurons damage might be through mediating NLRP3 inflammasome.展开更多
Measuring system is the core part of a space vehicle, and its integrated test is the important means of conducting system-level verification. For the integrated test items and flow, the solution featuring the automati...Measuring system is the core part of a space vehicle, and its integrated test is the important means of conducting system-level verification. For the integrated test items and flow, the solution featuring the automatic test method, the combination of different test items, and the electronic data record is proposed in this paper. Applying the above-mentioned solution, the test efficiency could be improved by more than 30% and the safety of test operation could be also exalted. At the same time, the solution has good universality and could provide reference for other similar integrated tests.展开更多
It is a big topic on sleep research in China,current sleep research in China will be introduced from basic and clinic research in the paper.Basic researches Current sleep research in China began with the study on hybe...It is a big topic on sleep research in China,current sleep research in China will be introduced from basic and clinic research in the paper.Basic researches Current sleep research in China began with the study on hybernation using urchin at Peking University in the early 1950s.During 1960-2017,lots of agencies such as Shanghai Institute of Physiology of Academia Sinica,Institute of Psychology of Academia Sinica,Henan Medical University have accumulated large investigative and experimental data on sleep,but the most of those data focused on the descriptive researches of animal assortment(feline,dog,goat,mouce,rat and macque)and sleep classicification[1].展开更多
基金Key Prograns for Science and Technology Development of Chinese Transportation Industry( No. 2008-353-332-140)
文摘The influence of fly ash on the fresh properties, mechanical properties and carbonation properties were studied in this paper. The performance of a kind of curing agent which was applied to the hardened concrete surface was evaluated. Incorporating large volume of fly ash will risk the concrete carbonation. The curing agent could prevent the concrete carbonation, and the mechanism was explained.
基金National Science and Technology Support Program of China ( No. 2009BAG15B02)
文摘Taizhou Yangtze River Highway Bridge is a large span suspension bridge with three pylons. The elastic cables are installed to connect the steel tower and the steel box girder. The constraints can increase the safety coefficient of the middle saddle, and improve the stress conditions of the middle pylon and decrease the deflection in the middle of the main girder, as well as the longitudinal displacement of the main girder caused by live loads. The anchorage boxes of the elastic cable are installed in the wind fairing outside the vertical web plate of the box girder. Two anchor boxes form a pair and are arranged parallelly. Eight anchor boxes are installed in the bridge. In this paper, the design scheme and the technical difficulties in manufacturing are briefly discussed with the precision control techniques.
基金National Natural Science Foundation of China(8146055681760658).
文摘OBJECTIVE To research the effect of naringenin(NAR) on LPS-induced dopaminergic neurons damage and its potential mechanism.METHODS Rats were randomly divided into the following six groups(n=10):control(0.9% NaCl),NAR alone(100 mg·kg-1),LPS(5 μg),LPS+NAR(50 mg·kg-1) and LPS+NAR(100 mg·kg-1).Rats were received a single LPS unilateral injection into the SN pars compacts,after seven daily intragastric administration of NAR,rats′ behavior was analyzed by rotarod test.Then,the expression of TH,IBA-1 and NLRP3 inflammasome were analyzed by Western blotting and immunofluorescence.In vitro experiments,BV-2 cel s were treated with different doses of NAR,and 1 h later,LPS(1 g·L^(-1)) was added to the medium for 24 h,then collect the culture medium and protein for later experiments.The production of IL-1β and IL-18 in culture medium were tested by ELISA,and the production of NO was detected by Griess reagent.The expression of IBA-1,NLRP3 and p-caspase 1 were detected by Western blotting.MN9 D cells were co-cultured with BV2 cells to mimic the animal experiments.MTT assay was used to analyzed the viability of MN9 D cells,and the expression of TH was detected by Western blotting.RESULTS NAR(100 mg · kg-1) could significantly improve the time of rats on the rotating(116.73 s vs 185.45 s,P<0.05).The result of the pathological analysis also suggested that NAR could decrease the activation of microglia as well as the expression of NLRP3 Inflammasome.In addition,NAR also could suppress the expression of pro-inflammatory factor levels,such as IL-1β(P<0.05),IL-18(P<0.05),and the protection of NAR could be inhibited by siR NA NLRP3.Moreover,an in vitro co-culture system with BV2 and MN9 D cells wasused to find the protection of NAR must via microglia,while there is no effect of NAR were directly added to MN9 D cells.CONCLUSION NAR protection of LPS-induced dopaminergic neurons damage might be through mediating NLRP3 inflammasome.
文摘Measuring system is the core part of a space vehicle, and its integrated test is the important means of conducting system-level verification. For the integrated test items and flow, the solution featuring the automatic test method, the combination of different test items, and the electronic data record is proposed in this paper. Applying the above-mentioned solution, the test efficiency could be improved by more than 30% and the safety of test operation could be also exalted. At the same time, the solution has good universality and could provide reference for other similar integrated tests.
文摘It is a big topic on sleep research in China,current sleep research in China will be introduced from basic and clinic research in the paper.Basic researches Current sleep research in China began with the study on hybernation using urchin at Peking University in the early 1950s.During 1960-2017,lots of agencies such as Shanghai Institute of Physiology of Academia Sinica,Institute of Psychology of Academia Sinica,Henan Medical University have accumulated large investigative and experimental data on sleep,but the most of those data focused on the descriptive researches of animal assortment(feline,dog,goat,mouce,rat and macque)and sleep classicification[1].
