Ciliated microeukaryotes(ciliates)are distinguished by their cilia,which are rich in tubulin.We developed a method for tubulin staining in ciliate cells that involves using live-cell tubulin-staining dyes instead of a...Ciliated microeukaryotes(ciliates)are distinguished by their cilia,which are rich in tubulin.We developed a method for tubulin staining in ciliate cells that involves using live-cell tubulin-staining dyes instead of antibodies thereby streamlining the staining process,which is effective across diverse ciliate lineages.Moreover,our method allows integration with immunofluorescence staining using antibodies when needed.The potential applications of this technique extend to cell biology and ciliate morphological and ecological studies.展开更多
基金supported by the Third Xinjiang Scientific Expedition Program(2021xjkk0204,2021xjkk0604)Science&Technology Fundamental Resources Investigation Program(2022FY100400)+2 种基金Background Resources Survey in Shennongjia National Park(SNJNP2022008)Hubei Provincial Key Laboratory for Conservation Biology of Shennongjia Snub-nosed Monkeys(SNJGKL2022008)Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation,Beibu Gulf University(2020KA05).
文摘Ciliated microeukaryotes(ciliates)are distinguished by their cilia,which are rich in tubulin.We developed a method for tubulin staining in ciliate cells that involves using live-cell tubulin-staining dyes instead of antibodies thereby streamlining the staining process,which is effective across diverse ciliate lineages.Moreover,our method allows integration with immunofluorescence staining using antibodies when needed.The potential applications of this technique extend to cell biology and ciliate morphological and ecological studies.
