Zeolitic imidazole framework-8(ZIF-8)particles,composed of zinc ions(Zn^(2+))and 2-methylimidazolate,were used as carriers for incorporating iron oxide(Fe_(3)O_(4))nanoparticles,resulting in Fe_(3)O_(4)@ZIF-8 particle...Zeolitic imidazole framework-8(ZIF-8)particles,composed of zinc ions(Zn^(2+))and 2-methylimidazolate,were used as carriers for incorporating iron oxide(Fe_(3)O_(4))nanoparticles,resulting in Fe_(3)O_(4)@ZIF-8 particles.Due to the toxicity of Zn^(2+)to cell membranes,liposomes were employed to reduce this toxicity.Fluorescent dyes were loaded into ZIF-8 or Fe_(3)O_(4)@ZIF-8 nanoparticles as the mock drugs to facilitate tracking during cellular studies.The encapsulation efficiency of fluorescein(Flu)and nile red(NiR)in the MOFs was calculated to be around40%-60%.A burst release of Flu was observed under acidic conditions within 30 min,while natural PBS was significantly release in 6 h.The release kinetic of the whole platform was fixed as the Higuchi equation which referred to diffusion release.Liposome coating significantly decreased the toxicity of the MOFs,as evidenced by an increase in IC_(50)values from approximately 30 to 120μg/mL.The LDH release from L929 cells was confirmed when particles were used at exceeding 100μg/mL.The cellular uptake of the liposome-coated dye-loaded MOFs was confirmed after 3 hour-incubation.These findings suggested that liposome-coated MOFs could be served as an alternative carrier in biomedical engineering field.展开更多
基金the fundamental fund from the University of Phayao(FF67-1848/2567)the Unit of Excellence on Advanced Nanomaterials which was supported by the Thailand Science Research+1 种基金Innovation Fund and the University of Phayaosupported by Chiang Mai University。
文摘Zeolitic imidazole framework-8(ZIF-8)particles,composed of zinc ions(Zn^(2+))and 2-methylimidazolate,were used as carriers for incorporating iron oxide(Fe_(3)O_(4))nanoparticles,resulting in Fe_(3)O_(4)@ZIF-8 particles.Due to the toxicity of Zn^(2+)to cell membranes,liposomes were employed to reduce this toxicity.Fluorescent dyes were loaded into ZIF-8 or Fe_(3)O_(4)@ZIF-8 nanoparticles as the mock drugs to facilitate tracking during cellular studies.The encapsulation efficiency of fluorescein(Flu)and nile red(NiR)in the MOFs was calculated to be around40%-60%.A burst release of Flu was observed under acidic conditions within 30 min,while natural PBS was significantly release in 6 h.The release kinetic of the whole platform was fixed as the Higuchi equation which referred to diffusion release.Liposome coating significantly decreased the toxicity of the MOFs,as evidenced by an increase in IC_(50)values from approximately 30 to 120μg/mL.The LDH release from L929 cells was confirmed when particles were used at exceeding 100μg/mL.The cellular uptake of the liposome-coated dye-loaded MOFs was confirmed after 3 hour-incubation.These findings suggested that liposome-coated MOFs could be served as an alternative carrier in biomedical engineering field.
