This study investigated antibiotics in pig feed and drinking water and their excretion together with manure and wastewater at a typical pig farm in Thailand.Among the 21 antibiotics analyzed,tetracyclines,macrolides,b...This study investigated antibiotics in pig feed and drinking water and their excretion together with manure and wastewater at a typical pig farm in Thailand.Among the 21 antibiotics analyzed,tetracyclines,macrolides,b-lactams,aminoglycosides,quinolones,sulfonamides,pleuromutilin,and florfenicol classes,were found as active ingredients in feed and drinking water and excreted together with pig wastes.Among them,doxycycline,tilmicosin,and amoxicillin were detected higher concentrations in wastewater(12.9e459.1 mg L^(-1)),sediment(0.074e0.62 mg kg^(-1)),and pig manure(0.45e4.1 mg kg^(-1)).During wastewater treatment,those major antibiotics were remaining in pond water and settled sludge up to 79.5 mg L-1 and 24.2 mg kg^(-1),respectively.The normalized excretion mass of total antibiotics was determined as 11.2 mg head-1 day-1,contributed mainly from doxycycline(5.9 mg head-1 day-1),tilmicosin(2.8 mg head-1 day-1),and amoxicillin(2.2 mg head-1 day-1).Based on total pig production in Thailand,the excreted mass of antibiotics was estimated at 79.3 tons year-1.展开更多
Currently,antimicrobial drug resistance is a problem that deserves thoughtful consideration.Especially in the swine production industry,many pig farms tend to release wastewater into natural water sources,which villag...Currently,antimicrobial drug resistance is a problem that deserves thoughtful consideration.Especially in the swine production industry,many pig farms tend to release wastewater into natural water sources,which villagers use for their livelihood.This study aimed to demonstrate the occurrence of phenotypic and genotypic resistance characteristics of E.coli,as well as antibiotic residues in manure and wastewater on swine farms in Prachinburi Province,Thailand.Samples were collected in 2020 and 2021 during the rainy and summer seasons.The results of a questionnaire showed that farmers used antibiotics for disease treatment and prevention at a ratio of 2/4(50%).The most common antibiotic used was amoxicillin(4/4,100%),followed by Enrofloxacin(75%)and colistin(25%).The analysis of antibiotic residue in wastewater by Liquid Chromatography-Mass Spectrometry(LCMS,LC-MS)during the rainy season revealed that amoxicillin(54%)was the highest,followed by florfenicol(14%)and tiamulin(13%).While oxytetracycline(59%),amoxicillin(20%),and florfenicol(11%)were the highest in the summer.The study of Risk Quotient(RQ)indicated that water resources present a risk of antibiotic contamination by sulfonamides,trimethoprim,b-lactam,quinolones,and tetracycline at high levels(SRQ>1)during both seasons.The minimal inhibitory concentrations(MICs)were determined by an antibiotic susceptibility test(AST),analyzed by microdilution technique,and VITEK®2 Compact.The test result indicated that a total of 143 isolates were found in manure(45/143),pre-treatment(51/143),and final effluent(posttreatment,47/143).ESBL-positive resistance was detected at 13%.However,an immensely high percentage of antimicrobial resistance was found for ampicillin(90%),whereas imipenem did not show any drug resistance(0%).The average MIC value towards colistin in all samples was 2.71 mg/ml,while mcr1 was not found in any samples.Furthermore,MDR was expressed in E.coli at as high as 76.22%(109/143).The highest MDR pattern detected was AMP-TETeFFCeSXT.The PCR technique detected that the diversity and abundance of AMR genes were not significantly different from animal manure and wastewater at the swine farms.The bla-TEM was found more frequently than bla-PS.While the tetracycline group(tetA,tetB)and aminoglycoside(aadA1,aadA2,aadB)represented a major proportion,respectively.The PFGE study revealed the possibility of similar genetic morphology from the different isolates found in this study.展开更多
The removal efficacies of 19 antibiotics in different hospital wastewater treatment processes(WWTP)were investigated at various sizes of 60 hospitals across Thailand.The results showed slight differences in the total ...The removal efficacies of 19 antibiotics in different hospital wastewater treatment processes(WWTP)were investigated at various sizes of 60 hospitals across Thailand.The results showed slight differences in the total antibiotic removals(79e84%)among WWTP sizes.High antibiotics loading to all WWTP were amoxicillin,ciprofloxacin,tetracycline ampicillin,and norfloxacin,respectively.Amoxicillin and ampicillin were eliminated highly(90e99%)in every size of WWTP,while various efficacies showed for other antibiotics(0e93%).According to process comparison,activated sludge(CAS),oxidation ditch(OD),and sequencing batch reactor(SBR)systems showed similar removals of overall antibiotics(P>0.05).The attached growth systems such as submerged aerated fixed-film(SAFF)demonstrated relatively lower antibiotic removals.The natural processes such as aerated lagoon(AL),facultative pond(FP),and constructed wetland(CW)presented substantial biodegradation of some recalcitrant antibiotics such as fluoroquinolones,sulfamethoxazole,and trimethoprim.Post-chlorination provided additional elimination of some antibiotics such as amoxicillin and ampicillin.Finally,the top-five of antibiotics released into the water environment via discharged effluent are amoxicillin,tetracycline,ampicillin,ciprofloxacin,and imipenem,respectively,which mainly from the suspended growth systems.展开更多
基金the Office of National Higher Education Science Research and Innovation Policy Council via the Program Management Unit for Human Resource&Institutional Development,Research and Innovation(PMU-B),grant number B16F630088.
文摘This study investigated antibiotics in pig feed and drinking water and their excretion together with manure and wastewater at a typical pig farm in Thailand.Among the 21 antibiotics analyzed,tetracyclines,macrolides,b-lactams,aminoglycosides,quinolones,sulfonamides,pleuromutilin,and florfenicol classes,were found as active ingredients in feed and drinking water and excreted together with pig wastes.Among them,doxycycline,tilmicosin,and amoxicillin were detected higher concentrations in wastewater(12.9e459.1 mg L^(-1)),sediment(0.074e0.62 mg kg^(-1)),and pig manure(0.45e4.1 mg kg^(-1)).During wastewater treatment,those major antibiotics were remaining in pond water and settled sludge up to 79.5 mg L-1 and 24.2 mg kg^(-1),respectively.The normalized excretion mass of total antibiotics was determined as 11.2 mg head-1 day-1,contributed mainly from doxycycline(5.9 mg head-1 day-1),tilmicosin(2.8 mg head-1 day-1),and amoxicillin(2.2 mg head-1 day-1).Based on total pig production in Thailand,the excreted mass of antibiotics was estimated at 79.3 tons year-1.
文摘Currently,antimicrobial drug resistance is a problem that deserves thoughtful consideration.Especially in the swine production industry,many pig farms tend to release wastewater into natural water sources,which villagers use for their livelihood.This study aimed to demonstrate the occurrence of phenotypic and genotypic resistance characteristics of E.coli,as well as antibiotic residues in manure and wastewater on swine farms in Prachinburi Province,Thailand.Samples were collected in 2020 and 2021 during the rainy and summer seasons.The results of a questionnaire showed that farmers used antibiotics for disease treatment and prevention at a ratio of 2/4(50%).The most common antibiotic used was amoxicillin(4/4,100%),followed by Enrofloxacin(75%)and colistin(25%).The analysis of antibiotic residue in wastewater by Liquid Chromatography-Mass Spectrometry(LCMS,LC-MS)during the rainy season revealed that amoxicillin(54%)was the highest,followed by florfenicol(14%)and tiamulin(13%).While oxytetracycline(59%),amoxicillin(20%),and florfenicol(11%)were the highest in the summer.The study of Risk Quotient(RQ)indicated that water resources present a risk of antibiotic contamination by sulfonamides,trimethoprim,b-lactam,quinolones,and tetracycline at high levels(SRQ>1)during both seasons.The minimal inhibitory concentrations(MICs)were determined by an antibiotic susceptibility test(AST),analyzed by microdilution technique,and VITEK®2 Compact.The test result indicated that a total of 143 isolates were found in manure(45/143),pre-treatment(51/143),and final effluent(posttreatment,47/143).ESBL-positive resistance was detected at 13%.However,an immensely high percentage of antimicrobial resistance was found for ampicillin(90%),whereas imipenem did not show any drug resistance(0%).The average MIC value towards colistin in all samples was 2.71 mg/ml,while mcr1 was not found in any samples.Furthermore,MDR was expressed in E.coli at as high as 76.22%(109/143).The highest MDR pattern detected was AMP-TETeFFCeSXT.The PCR technique detected that the diversity and abundance of AMR genes were not significantly different from animal manure and wastewater at the swine farms.The bla-TEM was found more frequently than bla-PS.While the tetracycline group(tetA,tetB)and aminoglycoside(aadA1,aadA2,aadB)represented a major proportion,respectively.The PFGE study revealed the possibility of similar genetic morphology from the different isolates found in this study.
文摘The removal efficacies of 19 antibiotics in different hospital wastewater treatment processes(WWTP)were investigated at various sizes of 60 hospitals across Thailand.The results showed slight differences in the total antibiotic removals(79e84%)among WWTP sizes.High antibiotics loading to all WWTP were amoxicillin,ciprofloxacin,tetracycline ampicillin,and norfloxacin,respectively.Amoxicillin and ampicillin were eliminated highly(90e99%)in every size of WWTP,while various efficacies showed for other antibiotics(0e93%).According to process comparison,activated sludge(CAS),oxidation ditch(OD),and sequencing batch reactor(SBR)systems showed similar removals of overall antibiotics(P>0.05).The attached growth systems such as submerged aerated fixed-film(SAFF)demonstrated relatively lower antibiotic removals.The natural processes such as aerated lagoon(AL),facultative pond(FP),and constructed wetland(CW)presented substantial biodegradation of some recalcitrant antibiotics such as fluoroquinolones,sulfamethoxazole,and trimethoprim.Post-chlorination provided additional elimination of some antibiotics such as amoxicillin and ampicillin.Finally,the top-five of antibiotics released into the water environment via discharged effluent are amoxicillin,tetracycline,ampicillin,ciprofloxacin,and imipenem,respectively,which mainly from the suspended growth systems.
