The development of oleogels has received increasing attention due to their health benefits and potential to replace saturated or trans fats.This work reports a preparation of sinapine-protein oleogels using a capillar...The development of oleogels has received increasing attention due to their health benefits and potential to replace saturated or trans fats.This work reports a preparation of sinapine-protein oleogels using a capillary suspension method,comparing the effect of different concentrations of sinapine and the three proteins on the stability of the system.The results show that the incorporation of 4-8μg/mL of sinapine promoted the formation of capillary bridges between protein particles.The oil constraint capacity of WPI-oleogels is improved by about 20%due to the addition of sinapine.The structural integrity of the oleogels was highly dependent on the protein hydrophilic capacity and the concentration of secondary fluid-sinapine.In addition,the stabilized oleogels prepared by sinapine had high energy storage modulus and thixotropic recovery,indicating the stabilizing effect of sinapine.Thus,the interfacially adsorbed particles and the polymer gel network jointly contribute to the dense structure of the oleogels.That the preparation of sinapine-protein oleogels based on capillary suspension has potential applications in foods with adjustable rheological and textural properties.展开更多
This study employed a Caco-2 intestinal model to evaluate the impact of soybean oil and its fatty acid constituents(oleic acid(OA),linoleic acid(LA),and linolenic acid(LNA))on the intestinal absorption,transport,and t...This study employed a Caco-2 intestinal model to evaluate the impact of soybean oil and its fatty acid constituents(oleic acid(OA),linoleic acid(LA),and linolenic acid(LNA))on the intestinal absorption,transport,and toxicity of benzo[a]pyrene(BaP)and benzo[b]fluoranthene(BbF).Key findings demonstrated that dietary lipids significantly enhanced the bioavailability of polycyclic aromatic hydrocarbons(PAHs)through synergistic active-passive transport mechanisms.Specifically,oil carriers increased BaP absorption by 14-17%and BbF absorption by 12-15%compared to free PAHs.Lipid-carried PAHs induced concentration-dependent cytotox-icity characterized by progressive lipid accumulation,elevated oxidative stress,mitochondrial membrane po-tential collapse,and apoptosis.The carrier-mediated enhancement followed a consistent potency gradient:free PAHs<OA<LNA<LA<oil carriers,implying the synergistic effect of multiple fatty acids in the oil carrier.These findings establish a pathological cycle of lipid-enhanced PAH absorption→intestinal barrier damage→further PAH accumulation,providing the first experimental analysis of dietary lipid-mediated PAH bioavailability and direct evidence for assessing PAH exposure risks in lipid-rich foods.展开更多
基金supported by the Natural Science Foundation of Jiangsu Province(BK20230585)the National Natural Science Foundation of China(NSFC,Grant No.32302006)+2 种基金the Natural Science Foundation of Jiangsu Province(BK20220586)the National Natural Science Foundation of China(NSFC,Grant No.32201976)the highlevel talent research start-up fund of Yangzhou University(137012624).
文摘The development of oleogels has received increasing attention due to their health benefits and potential to replace saturated or trans fats.This work reports a preparation of sinapine-protein oleogels using a capillary suspension method,comparing the effect of different concentrations of sinapine and the three proteins on the stability of the system.The results show that the incorporation of 4-8μg/mL of sinapine promoted the formation of capillary bridges between protein particles.The oil constraint capacity of WPI-oleogels is improved by about 20%due to the addition of sinapine.The structural integrity of the oleogels was highly dependent on the protein hydrophilic capacity and the concentration of secondary fluid-sinapine.In addition,the stabilized oleogels prepared by sinapine had high energy storage modulus and thixotropic recovery,indicating the stabilizing effect of sinapine.Thus,the interfacially adsorbed particles and the polymer gel network jointly contribute to the dense structure of the oleogels.That the preparation of sinapine-protein oleogels based on capillary suspension has potential applications in foods with adjustable rheological and textural properties.
基金supported by the Program of the National Natural Science Foundation of China(32572706,32101899,32272448,31972039,and 31201379).
文摘This study employed a Caco-2 intestinal model to evaluate the impact of soybean oil and its fatty acid constituents(oleic acid(OA),linoleic acid(LA),and linolenic acid(LNA))on the intestinal absorption,transport,and toxicity of benzo[a]pyrene(BaP)and benzo[b]fluoranthene(BbF).Key findings demonstrated that dietary lipids significantly enhanced the bioavailability of polycyclic aromatic hydrocarbons(PAHs)through synergistic active-passive transport mechanisms.Specifically,oil carriers increased BaP absorption by 14-17%and BbF absorption by 12-15%compared to free PAHs.Lipid-carried PAHs induced concentration-dependent cytotox-icity characterized by progressive lipid accumulation,elevated oxidative stress,mitochondrial membrane po-tential collapse,and apoptosis.The carrier-mediated enhancement followed a consistent potency gradient:free PAHs<OA<LNA<LA<oil carriers,implying the synergistic effect of multiple fatty acids in the oil carrier.These findings establish a pathological cycle of lipid-enhanced PAH absorption→intestinal barrier damage→further PAH accumulation,providing the first experimental analysis of dietary lipid-mediated PAH bioavailability and direct evidence for assessing PAH exposure risks in lipid-rich foods.
