In this study, we conducted a clinical analysis of lymphocyte subtypes in 268 patients with Parkinson＇s disease （PD） to assess their clinical impact as a potential marker of advanced PD in Chinese patients. The par...In this study, we conducted a clinical analysis of lymphocyte subtypes in 268 patients with Parkinson＇s disease （PD） to assess their clinical impact as a potential marker of advanced PD in Chinese patients. The participants comprised 268 sporadic PD patients and 268 healthy controls. The numbers of natural killer （NK） cells and CD3＋, CD3＋CD4＋, CD3＋CD8＋, and CD19＋ lympho- cytes from peripheral blood were determined by immunos- taining and flow cytometric analysis and the percentages of these CD＋ T cells were calculated. The ratio of regulatory T （Treg）/helper T 17 （Thl7） lymphocytes from 64 PD patients and 46 controls was determined by flow cytometric analysis. The results showed that the percentage of NK cells was higher in advanced PD patients than in controls （22.92% ± 10.08% versus 19.76% ±10.09%, P= 0.006）, while CD3＋ T cells are decreased （62.93% ±9.27% versus 65.75% ± 9.13%, P = 0.005）. The percentage of CD19＋ B cells in male patients was lower （P = 0.021） than in female patients, whereas NK cells were increased （P 〈 0.0001）. The scores on the Unified Parkinson＇s Disease Rating Scale （UPDRS） and the Non-Motor Symptoms Scale in late-onset PD patients were significantly higher than those in earlyonset patients （P = 0.024 and P = 0.007, respectively）. The percentage of CD19＋ B cells in patients with UPDRS scores 〉24 was lower than in those with scores 〈24 （10.17% ± 4.19% versus 12.22% ± 5.39%, P = 0.009）. In addition, the Treg/Th17 ratio in female patients was higher than that in female controls （13.88 ± 6.32 versus 9.94 ±4.06, P = 0.042）. These results suggest that the percentages of NK cells, CD3＋ T cells, and CD19＋ B cells along with the Treg/Th17 ratio in peripheral blood may be used to predict the risk of PD in Chinese individuals and provide fresh avenues for novel diagnostic biomarkers and therapeutic designs.展开更多
It is conducive to the sustainable development of human beings in karst regions to research the mechanism of karst rocky desertification(KRD)expansion.Whether the large-scale KRD in southwestern China is caused by cli...It is conducive to the sustainable development of human beings in karst regions to research the mechanism of karst rocky desertification(KRD)expansion.Whether the large-scale KRD in southwestern China is caused by climate change or human activities is still controversial.In this study,the evolution of the KRD in southwestern China over the past 2000 years was reconstructed through the high-precisionδ^(13)C record of stalagmites from Shijiangjun(SJJ)Cave,Guizhou Province,China.Theδ^(13)C of the stalagmites from SJJ Cave exhibited heavy values from the Medieval Warm Period(MWP)to the Little Ice Age(LIA).Furthermore,theδ^(13)C records of other stalagmites and tufa from southwestern China also showed the same significant heavy trend.Because the stalagmiteδ^(13)C could record the change of ecological environment,it indicated that the consistent change of the stalagmitesδ^(13)C may record the process of KRD expansion in the karst regions of southwestern China.During the MWP,the stronger Asian summer monsoon and the northward movement of the rain belt led to a dry period in southwestern China and a wet period in northern China.In contrast,it was wet in southwestern China and dry in northern China during the LIA.In addition,after the Jing-Kang event(JK event,AD1127)occurred at the end of the Northern Song dynasty,the political and economic center of China migrated to southern China for the first time,which changed the population distribution pattern of larger population in the north and smaller population in the south.Therefore,the expansion of KRD in southwestern China was exacerbated in the MWP due to the change of climate in southwestern China,the migration of a large number of people,wars,the large-scale reclamation of arable land,and the cultivation of large areas of crops.展开更多
基金supported by the National Key R&D Program of China (2016YFC1306600)the National Natural Science Foundation of China (81471292, U1603281, U1503222, 81430021)+2 种基金the Science Foundation of Guangdong Province, China (2015A030311021)a Technology Project of Guangzhou Municipality, China (201504281820463)a Science and Technology Planning Project of Guangdong Province, China (2016A050502025)
文摘In this study, we conducted a clinical analysis of lymphocyte subtypes in 268 patients with Parkinson＇s disease （PD） to assess their clinical impact as a potential marker of advanced PD in Chinese patients. The participants comprised 268 sporadic PD patients and 268 healthy controls. The numbers of natural killer （NK） cells and CD3＋, CD3＋CD4＋, CD3＋CD8＋, and CD19＋ lympho- cytes from peripheral blood were determined by immunos- taining and flow cytometric analysis and the percentages of these CD＋ T cells were calculated. The ratio of regulatory T （Treg）/helper T 17 （Thl7） lymphocytes from 64 PD patients and 46 controls was determined by flow cytometric analysis. The results showed that the percentage of NK cells was higher in advanced PD patients than in controls （22.92% ± 10.08% versus 19.76% ±10.09%, P= 0.006）, while CD3＋ T cells are decreased （62.93% ±9.27% versus 65.75% ± 9.13%, P = 0.005）. The percentage of CD19＋ B cells in male patients was lower （P = 0.021） than in female patients, whereas NK cells were increased （P 〈 0.0001）. The scores on the Unified Parkinson＇s Disease Rating Scale （UPDRS） and the Non-Motor Symptoms Scale in late-onset PD patients were significantly higher than those in earlyonset patients （P = 0.024 and P = 0.007, respectively）. The percentage of CD19＋ B cells in patients with UPDRS scores 〉24 was lower than in those with scores 〈24 （10.17% ± 4.19% versus 12.22% ± 5.39%, P = 0.009）. In addition, the Treg/Th17 ratio in female patients was higher than that in female controls （13.88 ± 6.32 versus 9.94 ±4.06, P = 0.042）. These results suggest that the percentages of NK cells, CD3＋ T cells, and CD19＋ B cells along with the Treg/Th17 ratio in peripheral blood may be used to predict the risk of PD in Chinese individuals and provide fresh avenues for novel diagnostic biomarkers and therapeutic designs.
基金supported by the National Natural Science Foundation of China(Grant Nos.41772170,42011530078)the Fundamental Research Funds for the Central Universities,China(Grant Nos.XDJK2017A010,XDJK2020D005)to Li T Y+2 种基金the Chongqing Municipal Science and Technology Commission Fellowship Fund(Grant Nos.cstc2019yszx-jcyjX0002,cstc2020yszxjcyjX0006)to Yuan D X,and the Open Project of Guangxi Key Science and Technology Innovation Base on Karst Dynamics(Grant No.KDL&Guangxi 202003)to Li J Y.230Th dating at the High-precision Mass Spectrometry and Environment Change Laboratory(HISPEC),Taiwan University,Chinawas supported by the Science Vanguard Research Program of the Ministry of Science and Technology(Grant No.108-2119-M-002-012)the Higher Education Sprout Project of the Ministry of Education,Taiwan,China(Grant No.108L901001)to Shen C C.This research was also Supported by the China Scholarship Council(CSC)(Grant No.202006990068)to Chen C C.
文摘It is conducive to the sustainable development of human beings in karst regions to research the mechanism of karst rocky desertification(KRD)expansion.Whether the large-scale KRD in southwestern China is caused by climate change or human activities is still controversial.In this study,the evolution of the KRD in southwestern China over the past 2000 years was reconstructed through the high-precisionδ^(13)C record of stalagmites from Shijiangjun(SJJ)Cave,Guizhou Province,China.Theδ^(13)C of the stalagmites from SJJ Cave exhibited heavy values from the Medieval Warm Period(MWP)to the Little Ice Age(LIA).Furthermore,theδ^(13)C records of other stalagmites and tufa from southwestern China also showed the same significant heavy trend.Because the stalagmiteδ^(13)C could record the change of ecological environment,it indicated that the consistent change of the stalagmitesδ^(13)C may record the process of KRD expansion in the karst regions of southwestern China.During the MWP,the stronger Asian summer monsoon and the northward movement of the rain belt led to a dry period in southwestern China and a wet period in northern China.In contrast,it was wet in southwestern China and dry in northern China during the LIA.In addition,after the Jing-Kang event(JK event,AD1127)occurred at the end of the Northern Song dynasty,the political and economic center of China migrated to southern China for the first time,which changed the population distribution pattern of larger population in the north and smaller population in the south.Therefore,the expansion of KRD in southwestern China was exacerbated in the MWP due to the change of climate in southwestern China,the migration of a large number of people,wars,the large-scale reclamation of arable land,and the cultivation of large areas of crops.
