Grapevine powdery mildew is caused by Erysiphe necator,which seriously harms grape production in the world.Stilbene synthase makes phytoalexins that contribute to the resistance of grapevine against powdery mildew.A n...Grapevine powdery mildew is caused by Erysiphe necator,which seriously harms grape production in the world.Stilbene synthase makes phytoalexins that contribute to the resistance of grapevine against powdery mildew.A novel VqNSTS3 was identified and cloned from Chinese wild Vitis quinquangularis accession Danfeng-2.The novel VqNSTS3 was transferred into susceptible‘Thompson Seedless’by Agrobacterium-mediated transformation.The transgenic plants showed resistance to the disease and activated other resistance-related genes.VqNSTS3 expression in grapevine is regulated by VqWRKY33,and which binds to TTGACC in the VqNSTS3 promoter.Furthermore,VqWRKY33 was phosphorylated by VqMAPK3/VqMAPK6 and thus led to enhanced signal transduction and increased VqNSTS3 expression.ProVqNSTS3::VqNSTS3-GFP of transgenic VqNSTS3 in Arabidopsis thaliana was observed to move to and wrap the pathogen’s haustoria and block invasion by Golovinomyces cichoracearum.These results demonstrate that stilbene accumulation of novel VqNSTS3 of the Chinese wild Vitis quinquangularis accession Danfeng-2 prevented pathogen invasion and enhanced resistance to powdery mildew.Therefore,VqNSTS3 can be used in generating powdery mildew-resistant grapevines.展开更多
Erysiphe necator is a destructive fungal pathogen that compromises grapevine yield and quality,leading to substantial economic losses.Therefore,elucidating host resistance mechanisms is essential.In this study,we iden...Erysiphe necator is a destructive fungal pathogen that compromises grapevine yield and quality,leading to substantial economic losses.Therefore,elucidating host resistance mechanisms is essential.In this study,we identified an ethylene response factor,VqERF1B,that exhibits sustained high expression during E.necator infection in Chinese wild grape Vitis quinquangularis accession ‘Danfeng-2'.Transient overexpression of VqERF1B in grape leaves enhanced resistance to E.necator by elevating transcript levels of pathogenesis-related(PR) genes,including PR1,PR2,PR5,and PR10.Conversely,silencing VqERF1B resulted in increased susceptibility.Moreover,transgenic Arabidopsis lines stably overexpressing VqERF1B exhibited enhanced resistance to powdery mildew,associated with elevated PR gene expression and increased accumulation of reactive oxygen species(ROS).A series of assays identified VqMAPK3,a phosphorylated mitogen-activated protein kinase,as a direct interactor of VqERF1B.Furthermore,VqERF1B was shown to bind directly to the promoters of VqPRs,thereby activating their transcription.Notably,the VqMAPK3-VqERF1B complex exhibited greater transactivation activity on VqPR promoters than VqERF1B alone,indicating that VqMAPK3 positively modulates VqERF1Bmediated transcription of PR genes.This work advances understanding of the molecular basis of grape resistance to E.necator and provides a foundation for molecular breeding strategies.展开更多
基金The research was funded by the National Natural Science Foundation of China(grant no.32272667).
文摘Grapevine powdery mildew is caused by Erysiphe necator,which seriously harms grape production in the world.Stilbene synthase makes phytoalexins that contribute to the resistance of grapevine against powdery mildew.A novel VqNSTS3 was identified and cloned from Chinese wild Vitis quinquangularis accession Danfeng-2.The novel VqNSTS3 was transferred into susceptible‘Thompson Seedless’by Agrobacterium-mediated transformation.The transgenic plants showed resistance to the disease and activated other resistance-related genes.VqNSTS3 expression in grapevine is regulated by VqWRKY33,and which binds to TTGACC in the VqNSTS3 promoter.Furthermore,VqWRKY33 was phosphorylated by VqMAPK3/VqMAPK6 and thus led to enhanced signal transduction and increased VqNSTS3 expression.ProVqNSTS3::VqNSTS3-GFP of transgenic VqNSTS3 in Arabidopsis thaliana was observed to move to and wrap the pathogen’s haustoria and block invasion by Golovinomyces cichoracearum.These results demonstrate that stilbene accumulation of novel VqNSTS3 of the Chinese wild Vitis quinquangularis accession Danfeng-2 prevented pathogen invasion and enhanced resistance to powdery mildew.Therefore,VqNSTS3 can be used in generating powdery mildew-resistant grapevines.
基金This study is granted by the National Natural Science Foundation of China(52209055,52379041,and 32272667)the Yunnan Fundamental Research Projects,China (202401AU070197,202501AW070013,202501BC070015,and 202501AT070377)+3 种基金the Yunnan Education Department Project,China (2024J0079)the Kunming University of Science and Technology Talent Development Project,China (KKZ3202423161)the Yunnan Key Laboratory of Efficient Utilization of Agricultural Water Resources and Intelligent Control,China (202449CE340014)the Yunnan Intelligent Water-Fertilizer-Pesticide Integration Technology and Equipment Innovation Team,China (202505AS350025)。
文摘Erysiphe necator is a destructive fungal pathogen that compromises grapevine yield and quality,leading to substantial economic losses.Therefore,elucidating host resistance mechanisms is essential.In this study,we identified an ethylene response factor,VqERF1B,that exhibits sustained high expression during E.necator infection in Chinese wild grape Vitis quinquangularis accession ‘Danfeng-2'.Transient overexpression of VqERF1B in grape leaves enhanced resistance to E.necator by elevating transcript levels of pathogenesis-related(PR) genes,including PR1,PR2,PR5,and PR10.Conversely,silencing VqERF1B resulted in increased susceptibility.Moreover,transgenic Arabidopsis lines stably overexpressing VqERF1B exhibited enhanced resistance to powdery mildew,associated with elevated PR gene expression and increased accumulation of reactive oxygen species(ROS).A series of assays identified VqMAPK3,a phosphorylated mitogen-activated protein kinase,as a direct interactor of VqERF1B.Furthermore,VqERF1B was shown to bind directly to the promoters of VqPRs,thereby activating their transcription.Notably,the VqMAPK3-VqERF1B complex exhibited greater transactivation activity on VqPR promoters than VqERF1B alone,indicating that VqMAPK3 positively modulates VqERF1Bmediated transcription of PR genes.This work advances understanding of the molecular basis of grape resistance to E.necator and provides a foundation for molecular breeding strategies.
