In anurans,advertisement calls(ACs)are an essential form of intraspecific communication.This study evaluates geographical variation in the ACs of Leptobrachella ventripunctata in the Guizhou Plateau,southwestern China...In anurans,advertisement calls(ACs)are an essential form of intraspecific communication.This study evaluates geographical variation in the ACs of Leptobrachella ventripunctata in the Guizhou Plateau,southwestern China,and explores correlations between call characteristics,body size,and environmental factors.ACs are simple calls of L.ventripunctata,and apparent differences were observed in the ACs among different geographical populations of L.ventripunctata.The Call duration(CD)revealed a significant positive correlation with altitude and a significant negative correlation with temperature and humidity.Moreover,the Dominant frequency(DF)exhibited a significant negative correlation with altitude and the habitat closure degree and a significant positive correlation with temperature.These variations in ACs between different geographical populations of L.ventripunctata may critically impact the adaptive evolution of species,and the calls may also be relevant for environmental selection.展开更多
Mineralization has found widespread use in the fabrication of composite biomaterials for hard tissue regeneration.The current mineralization processes are mainly carried out in neutral aqueous solutions of biomineral ...Mineralization has found widespread use in the fabrication of composite biomaterials for hard tissue regeneration.The current mineralization processes are mainly carried out in neutral aqueous solutions of biomineral counter-ions(a pair of cation and anion that form the corresponding minerals at certain conditions),which are stable only at very low concentrations.This typically results in inefficient mineralization and weak control over biomineral formation.Here,we find that,in the organic solvent glycerol,a variety of biomineral counter-ions(e.g.,Ca/PO_(4),Ca/CO_(3),Ca/SO_(4),Mg/PO_(4),or Fe/OH)corresponding to distinct biominerals at significantly high concentrations(up to hundreds-fold greater than those of simulated body fluid(SBF))are able to form translucent and stable solutions(mineralizing solution of highly concentrated counter-ions(MSCIs)),and mineralization can be triggered upon them with external solvents(e.g.,water or ethanol).Furthermore,with pristine bacterial cellulose(BC)membrane as a model,we demonstrate an effective and controllable mineralization performance of MSCIs on organic substrates.This approach not only forms the homogeneous biominerals on the BC fibers and in the interspaces,but also provides regulations over mineralization rate,mineral content,phase,and dopants.The resulting mineralized BC membranes(MBCs)exhibit high cytocompatibility and favor the proliferation of rat bone marrow mesenchymal stem cells(rBMSC).Following this,we prepare a mineralized bone suture(MBS)from MBC for non-weight bearing bone fixation,which then is tested on a rabbit median sternotomy model.It shows firm fixation of the rabbit sternum without causing discernible toxicity or inflammatory response.This study,by extending the mineralization to the organic solution system of highly concentrated counter-ions,develops a promising strategy to design and build targeted mineral-based composites.展开更多
基金supported by the National Natural Science Foundation of China(32060307 and 31860610)Guizhou Provincial Science and Technology Planning Project[[2021]500].
文摘In anurans,advertisement calls(ACs)are an essential form of intraspecific communication.This study evaluates geographical variation in the ACs of Leptobrachella ventripunctata in the Guizhou Plateau,southwestern China,and explores correlations between call characteristics,body size,and environmental factors.ACs are simple calls of L.ventripunctata,and apparent differences were observed in the ACs among different geographical populations of L.ventripunctata.The Call duration(CD)revealed a significant positive correlation with altitude and a significant negative correlation with temperature and humidity.Moreover,the Dominant frequency(DF)exhibited a significant negative correlation with altitude and the habitat closure degree and a significant positive correlation with temperature.These variations in ACs between different geographical populations of L.ventripunctata may critically impact the adaptive evolution of species,and the calls may also be relevant for environmental selection.
基金supported by the National Key R&D Program of China(No.2022YFE0123500)the National Natural Science Foundation of China(Nos.52272304 and 31771081)Science and Technology Commission of Shanghai Municipality(Nos.21ZR1449700,22S31903300,and 22S31900100).
文摘Mineralization has found widespread use in the fabrication of composite biomaterials for hard tissue regeneration.The current mineralization processes are mainly carried out in neutral aqueous solutions of biomineral counter-ions(a pair of cation and anion that form the corresponding minerals at certain conditions),which are stable only at very low concentrations.This typically results in inefficient mineralization and weak control over biomineral formation.Here,we find that,in the organic solvent glycerol,a variety of biomineral counter-ions(e.g.,Ca/PO_(4),Ca/CO_(3),Ca/SO_(4),Mg/PO_(4),or Fe/OH)corresponding to distinct biominerals at significantly high concentrations(up to hundreds-fold greater than those of simulated body fluid(SBF))are able to form translucent and stable solutions(mineralizing solution of highly concentrated counter-ions(MSCIs)),and mineralization can be triggered upon them with external solvents(e.g.,water or ethanol).Furthermore,with pristine bacterial cellulose(BC)membrane as a model,we demonstrate an effective and controllable mineralization performance of MSCIs on organic substrates.This approach not only forms the homogeneous biominerals on the BC fibers and in the interspaces,but also provides regulations over mineralization rate,mineral content,phase,and dopants.The resulting mineralized BC membranes(MBCs)exhibit high cytocompatibility and favor the proliferation of rat bone marrow mesenchymal stem cells(rBMSC).Following this,we prepare a mineralized bone suture(MBS)from MBC for non-weight bearing bone fixation,which then is tested on a rabbit median sternotomy model.It shows firm fixation of the rabbit sternum without causing discernible toxicity or inflammatory response.This study,by extending the mineralization to the organic solution system of highly concentrated counter-ions,develops a promising strategy to design and build targeted mineral-based composites.
