Protein nanotubes(PNTs)can be regarded as two-dimensional(2D)lattices with p1 or p2 symmetry rolled into tubes.However,attempts to re-assemble their building blocks into stable 2D nanomaterials often fail.Here,startin...Protein nanotubes(PNTs)can be regarded as two-dimensional(2D)lattices with p1 or p2 symmetry rolled into tubes.However,attempts to re-assemble their building blocks into stable 2D nanomaterials often fail.Here,starting from two baculoviral capsid proteins,we screened protein variants for the in vitro assembly of various nanotubes and nanosheets.These high-order assemblies were structurally characterized by cryo-electron microscopy techniques.Interfacial analysis of three groups of PNTs revealed that helical heterogeneity is largely the result of the redundancy of p2 symmetry-related contacting interfaces.The assembled nanosheets showed similar interfacial networks to their nanotubular counterparts.In addition,foreign macromolecules could be efficiently displayed on the sizecontrollable double-layered nanosheets.This study sheds light on the rational design of flexible nanosheets,and it also provides novel 2D protein scaffolds for developing biocompatible materials.展开更多
Oasis stability in the lower reaches of the Tarim River was assessed at the landscape scale（including changes in landscape pattern and land use in the oasis） and at the regional scale（including the cold-island effe...Oasis stability in the lower reaches of the Tarim River was assessed at the landscape scale（including changes in landscape pattern and land use in the oasis） and at the regional scale（including the cold-island effect and NDVI of the oasis） based on the methods of landscape ecology,GIS and Remote Sensing（RS）.Thematic Mapping（TM） remote sensing images from 1990,2000 and 2009 were employed along with the related meteorological and hydrological data pertaining to the reclamation area of the oasis in the lower reaches of the Tarim River.The results indicated that landscape heterogeneity and oasis complexity increased between 1990 and 2000.The comprehensive index of the degree of land use,the average index of the cold-island effect,NDVI value and oasis stability all decreased.However,the change trends in the indices referred to above were opposite between 2000 and 2009,and oasis stability was enhanced to some extent.展开更多
BACKGROUND The selection criteria for Barcelona Clinic Liver Cancer(BCLC)intermediate-stage hepatocellular carcinoma(HCC)patients who would truly benefit from liver resection(LR)remain undefined.AIM To identify BCLC-B...BACKGROUND The selection criteria for Barcelona Clinic Liver Cancer(BCLC)intermediate-stage hepatocellular carcinoma(HCC)patients who would truly benefit from liver resection(LR)remain undefined.AIM To identify BCLC-B HCC patients more suitable for LR.METHODS We included patients undergoing curative LR for BCLC stage A or B multinodular HCC(MNHCC)and stratified BCLC-B patients by the sum of tumor size and number(N+S).Overall survival(OS),recurrence-free survival(RFS),recurrence-to-death survival(RTDS),recurrence patterns,and treatments after recurrence in BCLC-B patients in each subgroup were compared with those in BCLC-A patients.RESULTS In total,143 patients who underwent curative LR for MNHCC with BCLC-A(n=25)or BCLC-B(n=118)were retrospectively analyzed.According to the N+S,patients with BCLC-B HCC were divided into two subgroups:BCLC-B1(N+S≤10,n=83)and BCLC-B2(N+S>10,n=35).Compared with BCLC-B2 patients,those with BCLC-B1 had a better OS(5-year OS rate:67.4%vs 33.6%;P<0.001),which was comparable to that in BCLC-A patients(5-year OS rate:67.4%vs 74.1%;P=0.250),and a better RFS(median RFS:19 mo vs 7 mo;P<0.001),which was worse than that in BCLC-A patients(median RFS:19 mo vs 48 mo;P=0.022).Further analysis of patients who developed recurrence showed that both BCLC-B1 and BCLC-A patients had better RTDS(median RTDS:Not reached vs 49 mo;P=0.599),while the RTDS in BCLC-B2 patients was worse(median RTDS:16 mo vs not reached,P<0.001;16 mo vs 49 mo,P=0.042).The recurrence patterns were similar between BCLC-B1 and BCLC-A patients,but BCLC-B2 patients had a shorter recurrence time and a higher proportion of patients had recurrence with macrovascular invasion and/or extrahepatic metastasis,both of which were independent risk factors for RTDS.CONCLUSION BCLC-B HCC patients undergoing hepatectomy with N+S≤10 had mild recurrence patterns and excellent OS similar to those in BCLC-A MNHCC patients,and LR should be considered in these patients.展开更多
BACKGROUND Gallbladder perforation is a serious complication of acute cholecystitis.Such perforation is a rare but life-threatening situation that can lead to the formation and rupture of liver hematomas.Here,we repor...BACKGROUND Gallbladder perforation is a serious complication of acute cholecystitis.Such perforation is a rare but life-threatening situation that can lead to the formation and rupture of liver hematomas.Here,we report a case of a ruptured intrahepatic hematoma caused by intrahepatic gallbladder perforation,and we present a literature review.CASE SUMMARY A 70-year-old male was admitted to the hospital with a complaint of right upper quadrant abdominal pain,flustering and dizziness.The preoperative diagnosis was a ruptured malignant liver tumor,and the patient’s medical images and increased level of carbohydrate antigen-199 suggested that the gallbladder had been invaded.However,the tumor was proven to be a liver hematoma secondary to gallbladder perforation after surgery.The patient was discharged uneventfully on the fifteenth postoperative day.CONCLUSION Intrahepatic gallbladder perforation is difficult to diagnose preoperatively.Radiological examinations play a crucial role in the diagnosis but only for partial cases.Early diagnosis and appropriate surgery are key to managing this rare condition.展开更多
[Objective]This study aimed to determine physiological and biochemical indices of Hailan chickens at 20 weeks of age.[Method]T,P,BT,CT,ESR,systolic pressure,diastolic pressure,RBC,HCT,MCV,RDW,HGB,MCH,MCHC,PLT,MPV,WBC,...[Objective]This study aimed to determine physiological and biochemical indices of Hailan chickens at 20 weeks of age.[Method]T,P,BT,CT,ESR,systolic pressure,diastolic pressure,RBC,HCT,MCV,RDW,HGB,MCH,MCHC,PLT,MPV,WBC,N,E,B,L,M,TP,Alb,AST,ALT,Glu and TG and CHO of 20 weeks-old male and female Hailan chickens were determined with various methods.[Result]According to the determined blood physiological and biochemical indices,RBC,HGB,Glu and ALB in male chickens were higher compared with female chickens,exhibiting extremely significant differences（P〈0.01）;B,M and TP in male chickens were significantly higher compared with female chickens（P〈0.05）;other physiological and biochemical indices exhibited no significant differences between males and females（P〉0.05）.[Conclusion]Physiological and biochemical indices of 20 weeks-old Hailan chickens varied between males and females,which were generally higher in male chickens than in female chickens.展开更多
The prevalence of quinolone antibiotics as emerging contaminants in wastewater necessitates urgent remediation strategies due to their recalcitrant nature and ecological risks.This study demonstrates an innovative Ag-...The prevalence of quinolone antibiotics as emerging contaminants in wastewater necessitates urgent remediation strategies due to their recalcitrant nature and ecological risks.This study demonstrates an innovative Ag-nZVI/BC composite synthesized through silver-modified nZVI immobilized on coconut shell biochar,which effectively activates H_(2)O_(2)for levofloxacin(LVF)degradation.The composite’s exceptional performance(91.2%LVF removal at 0.3 g/L dosage)stems from its 238 m^(2)/g specific surface area and abundant oxygen-containing functionalities that facilitate electron transfer.Systematic parameter optimization revealed pH 3.0,10 mmol/L H_(2)O_(2),and 0.3 g/L catalyst as optimal conditions,while coexisting anions showed differential inhibition effects.Radical quenching experiments coupled with EPR spectroscopy confirmed·OH and·O_(2)^(−)as dominant reactive species.Through LC-MS analysis,we identified three primary degradation pathways involving piperazine ring cleavage and defluorination.Notably,eco-toxicity assessment using ECOSAR indicated 62.7%reduction in acute aquatic toxicity after treatment.The regenerable catalyst maintained 83.4%efficiency after five cycles,demonstrating a sustainable approach for antibiotic-contaminated wastewater remediation.展开更多
基金supported by the National Key Research and Development Program of China(2022YFC2303300 to S.C.)the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB0490000 to S.C.)+1 种基金the Youth Innovation Promotion Association of the Chinese Academy of Sciences(2022341 to G.R.)the Major Project of the Guangzhou National Laboratory(GZNL2024A01010 to G.R.).
文摘Protein nanotubes(PNTs)can be regarded as two-dimensional(2D)lattices with p1 or p2 symmetry rolled into tubes.However,attempts to re-assemble their building blocks into stable 2D nanomaterials often fail.Here,starting from two baculoviral capsid proteins,we screened protein variants for the in vitro assembly of various nanotubes and nanosheets.These high-order assemblies were structurally characterized by cryo-electron microscopy techniques.Interfacial analysis of three groups of PNTs revealed that helical heterogeneity is largely the result of the redundancy of p2 symmetry-related contacting interfaces.The assembled nanosheets showed similar interfacial networks to their nanotubular counterparts.In addition,foreign macromolecules could be efficiently displayed on the sizecontrollable double-layered nanosheets.This study sheds light on the rational design of flexible nanosheets,and it also provides novel 2D protein scaffolds for developing biocompatible materials.
基金supported by the National Basic Research Program of China (2010CB951003)National Natural Science Foundation of China (40901061)+1 种基金Key Topics in Innovation Engineering of the Chinese Academy of Sciences (KZCX2-YW-Q10-3-4)Western Talent Cultivation Plan(XBBS200907)
文摘Oasis stability in the lower reaches of the Tarim River was assessed at the landscape scale（including changes in landscape pattern and land use in the oasis） and at the regional scale（including the cold-island effect and NDVI of the oasis） based on the methods of landscape ecology,GIS and Remote Sensing（RS）.Thematic Mapping（TM） remote sensing images from 1990,2000 and 2009 were employed along with the related meteorological and hydrological data pertaining to the reclamation area of the oasis in the lower reaches of the Tarim River.The results indicated that landscape heterogeneity and oasis complexity increased between 1990 and 2000.The comprehensive index of the degree of land use,the average index of the cold-island effect,NDVI value and oasis stability all decreased.However,the change trends in the indices referred to above were opposite between 2000 and 2009,and oasis stability was enhanced to some extent.
基金approved by the Ethics Committee of the Tongji Hospital of Tongji Medical College of Huazhong University of Science and Technology(Approval No.TJIRB20210918).
文摘BACKGROUND The selection criteria for Barcelona Clinic Liver Cancer(BCLC)intermediate-stage hepatocellular carcinoma(HCC)patients who would truly benefit from liver resection(LR)remain undefined.AIM To identify BCLC-B HCC patients more suitable for LR.METHODS We included patients undergoing curative LR for BCLC stage A or B multinodular HCC(MNHCC)and stratified BCLC-B patients by the sum of tumor size and number(N+S).Overall survival(OS),recurrence-free survival(RFS),recurrence-to-death survival(RTDS),recurrence patterns,and treatments after recurrence in BCLC-B patients in each subgroup were compared with those in BCLC-A patients.RESULTS In total,143 patients who underwent curative LR for MNHCC with BCLC-A(n=25)or BCLC-B(n=118)were retrospectively analyzed.According to the N+S,patients with BCLC-B HCC were divided into two subgroups:BCLC-B1(N+S≤10,n=83)and BCLC-B2(N+S>10,n=35).Compared with BCLC-B2 patients,those with BCLC-B1 had a better OS(5-year OS rate:67.4%vs 33.6%;P<0.001),which was comparable to that in BCLC-A patients(5-year OS rate:67.4%vs 74.1%;P=0.250),and a better RFS(median RFS:19 mo vs 7 mo;P<0.001),which was worse than that in BCLC-A patients(median RFS:19 mo vs 48 mo;P=0.022).Further analysis of patients who developed recurrence showed that both BCLC-B1 and BCLC-A patients had better RTDS(median RTDS:Not reached vs 49 mo;P=0.599),while the RTDS in BCLC-B2 patients was worse(median RTDS:16 mo vs not reached,P<0.001;16 mo vs 49 mo,P=0.042).The recurrence patterns were similar between BCLC-B1 and BCLC-A patients,but BCLC-B2 patients had a shorter recurrence time and a higher proportion of patients had recurrence with macrovascular invasion and/or extrahepatic metastasis,both of which were independent risk factors for RTDS.CONCLUSION BCLC-B HCC patients undergoing hepatectomy with N+S≤10 had mild recurrence patterns and excellent OS similar to those in BCLC-A MNHCC patients,and LR should be considered in these patients.
文摘BACKGROUND Gallbladder perforation is a serious complication of acute cholecystitis.Such perforation is a rare but life-threatening situation that can lead to the formation and rupture of liver hematomas.Here,we report a case of a ruptured intrahepatic hematoma caused by intrahepatic gallbladder perforation,and we present a literature review.CASE SUMMARY A 70-year-old male was admitted to the hospital with a complaint of right upper quadrant abdominal pain,flustering and dizziness.The preoperative diagnosis was a ruptured malignant liver tumor,and the patient’s medical images and increased level of carbohydrate antigen-199 suggested that the gallbladder had been invaded.However,the tumor was proven to be a liver hematoma secondary to gallbladder perforation after surgery.The patient was discharged uneventfully on the fifteenth postoperative day.CONCLUSION Intrahepatic gallbladder perforation is difficult to diagnose preoperatively.Radiological examinations play a crucial role in the diagnosis but only for partial cases.Early diagnosis and appropriate surgery are key to managing this rare condition.
文摘[Objective]This study aimed to determine physiological and biochemical indices of Hailan chickens at 20 weeks of age.[Method]T,P,BT,CT,ESR,systolic pressure,diastolic pressure,RBC,HCT,MCV,RDW,HGB,MCH,MCHC,PLT,MPV,WBC,N,E,B,L,M,TP,Alb,AST,ALT,Glu and TG and CHO of 20 weeks-old male and female Hailan chickens were determined with various methods.[Result]According to the determined blood physiological and biochemical indices,RBC,HGB,Glu and ALB in male chickens were higher compared with female chickens,exhibiting extremely significant differences（P〈0.01）;B,M and TP in male chickens were significantly higher compared with female chickens（P〈0.05）;other physiological and biochemical indices exhibited no significant differences between males and females（P〉0.05）.[Conclusion]Physiological and biochemical indices of 20 weeks-old Hailan chickens varied between males and females,which were generally higher in male chickens than in female chickens.
基金funding sources by the National Natural Science Foundation of China(No.52100071).
文摘The prevalence of quinolone antibiotics as emerging contaminants in wastewater necessitates urgent remediation strategies due to their recalcitrant nature and ecological risks.This study demonstrates an innovative Ag-nZVI/BC composite synthesized through silver-modified nZVI immobilized on coconut shell biochar,which effectively activates H_(2)O_(2)for levofloxacin(LVF)degradation.The composite’s exceptional performance(91.2%LVF removal at 0.3 g/L dosage)stems from its 238 m^(2)/g specific surface area and abundant oxygen-containing functionalities that facilitate electron transfer.Systematic parameter optimization revealed pH 3.0,10 mmol/L H_(2)O_(2),and 0.3 g/L catalyst as optimal conditions,while coexisting anions showed differential inhibition effects.Radical quenching experiments coupled with EPR spectroscopy confirmed·OH and·O_(2)^(−)as dominant reactive species.Through LC-MS analysis,we identified three primary degradation pathways involving piperazine ring cleavage and defluorination.Notably,eco-toxicity assessment using ECOSAR indicated 62.7%reduction in acute aquatic toxicity after treatment.The regenerable catalyst maintained 83.4%efficiency after five cycles,demonstrating a sustainable approach for antibiotic-contaminated wastewater remediation.
