Leaf thickness in rice critically influences photosynthetic efficiency and yield,yet its genetic basis remains poorly understood,with few functional genes previously characterized.In this study,we employ a pangenome-w...Leaf thickness in rice critically influences photosynthetic efficiency and yield,yet its genetic basis remains poorly understood,with few functional genes previously characterized.In this study,we employ a pangenome-wide association study(Pan-GWAS)on 302 diverse rice accessions from southern China,identifying 49 quantitative trait loci(QTLs)associated with leaf thickness.The most significant locus,qLT9,is fine-mapped to a 79-kb region on chromosome 9.Transcriptomic and genomic sequence analyses identify LOC_Os09g33480,which encodes a protein belonging to Multiple Organellar RNA Editing Factor family,as the key candidate gene.Overexpression and complementation transgenic experiments confirm LOC_Os09g33480(OsLT9)as the functional gene underlying qLT9,demonstrating a 24-bp Indel in its promoter correlates with the expression levels and leaf thickness.Notably,OsLT9 overexpression lines show not only thicker leaf,but also significantly enhanced photosynthetic efficiency and grain yield,establishing a link between leaf thickness modulation and yield enhancement.Population genomic analyses indicate strong selection for OsLT9 during domestication and breeding,with modern cultivars favoring thick leaf haplotype of OsLT9.This study establishes OsLT9 as a key regulator controlling leaf thickness in rice,and provides a valuable genetic resource for molecular breeding of high-yielding rice through optimization of plant architecture.展开更多
Dear Editor,Theanine(N-ethyl-γ-L-glutamine)is a characteristic amino acid primarily found in tea leaves(Camellia sinensis).It is derived from pyruvate through three enzymatic steps involving alanine aminotransferase(...Dear Editor,Theanine(N-ethyl-γ-L-glutamine)is a characteristic amino acid primarily found in tea leaves(Camellia sinensis).It is derived from pyruvate through three enzymatic steps involving alanine aminotransferase(AlaAT),alanine decarboxylase(AlaDC),and theanine synthetase(TS)(She et al.,2022;Luo and He,2025).Theanine has attracted widespread attention due to its tasteenhancing properties and potential health benefits,including relaxation and cognitive enhancement(Li et al.,2022),however,its limited natural production,coupled with increasing demand,has seriously restricted its broad application.展开更多
基金supported by the National Natural Science Foundation of China(32301845)GuangDong Basic and Applied Basic Research Foundation(2022A1515012339)+3 种基金the National Key R&D Program of China(2024YFD1200801)Seed industry revitalization project of special fund for rural revitalization strategy in Guangdong Province(2024-NPY-00-001)Modern Seed Industry Innovation Capacity Enhancement Progject of Guangdong Academy of Agricultural Sciences,Elite Rice Plan of GDRRI(2023YG01)Guangdong Key Laboratory of Rice Science and Technology(2023B1212060042).
文摘Leaf thickness in rice critically influences photosynthetic efficiency and yield,yet its genetic basis remains poorly understood,with few functional genes previously characterized.In this study,we employ a pangenome-wide association study(Pan-GWAS)on 302 diverse rice accessions from southern China,identifying 49 quantitative trait loci(QTLs)associated with leaf thickness.The most significant locus,qLT9,is fine-mapped to a 79-kb region on chromosome 9.Transcriptomic and genomic sequence analyses identify LOC_Os09g33480,which encodes a protein belonging to Multiple Organellar RNA Editing Factor family,as the key candidate gene.Overexpression and complementation transgenic experiments confirm LOC_Os09g33480(OsLT9)as the functional gene underlying qLT9,demonstrating a 24-bp Indel in its promoter correlates with the expression levels and leaf thickness.Notably,OsLT9 overexpression lines show not only thicker leaf,but also significantly enhanced photosynthetic efficiency and grain yield,establishing a link between leaf thickness modulation and yield enhancement.Population genomic analyses indicate strong selection for OsLT9 during domestication and breeding,with modern cultivars favoring thick leaf haplotype of OsLT9.This study establishes OsLT9 as a key regulator controlling leaf thickness in rice,and provides a valuable genetic resource for molecular breeding of high-yielding rice through optimization of plant architecture.
基金supported by the Key Areas Research Projects of Guangdong Province(2022B0202060002)the Incubation Program for the Science and Technology Development of Chinese Medicine Guangdong Laboratory(HQL2024PZ028)+4 种基金the Guangdong Basic and Applied Basic Research Foundation(2024A1515012120)the Guangzhou Basic and Applied Basic Research Foundation(2023A04J00359)the Special Foundation for Introduction of Scientific Talents of GDAAS(R2021YJ-YB3015,R2022YJ-YB1003,R2022PY-QY001,and R2021YJ-XD001)the Elite Rice Plan of GDRRI(2022YG01,2022YG02,and 2025YG02)the Guangdong Key Laboratory of Rice Science and Technology(2023B1212060042).
文摘Dear Editor,Theanine(N-ethyl-γ-L-glutamine)is a characteristic amino acid primarily found in tea leaves(Camellia sinensis).It is derived from pyruvate through three enzymatic steps involving alanine aminotransferase(AlaAT),alanine decarboxylase(AlaDC),and theanine synthetase(TS)(She et al.,2022;Luo and He,2025).Theanine has attracted widespread attention due to its tasteenhancing properties and potential health benefits,including relaxation and cognitive enhancement(Li et al.,2022),however,its limited natural production,coupled with increasing demand,has seriously restricted its broad application.
