The economic value of Fuzhuan brick tea(FBT)is closely related to the sexual development of Golden-flower fungus,yet the regulatory mechanism of sexual development in the fungus remains unclear.This study identified a...The economic value of Fuzhuan brick tea(FBT)is closely related to the sexual development of Golden-flower fungus,yet the regulatory mechanism of sexual development in the fungus remains unclear.This study identified a novel mating-type(MAT)gene AcMAT1-1-2 in Golden-flower fungus.The results showed that the deletion of AcMAT1-1-2 resulted in the minification of cleistothecium by 29.2%,the irregularity of colony morphology,the darkening of colony color,the highest number of ascomycetes and conidiophores unit area were each reduced by 16.6%;the interaction between AcMAT1-1-2 and AcMAT1-1-1 proteins;the AcMAT1-1-2 gene interacts with other related pathways by regulating the Biosynthesis of secondary metabolites pathway,which in turn regulates the mutant strains of AcMAT1-1-2 emerged new metabolites;the main differential metabolites have pyridines and derivatives,isoquinolines and derivatives,diazines,which may contribute to the formation of FBT quality and flavor,as well as anti-inflammatory,antioxidant and hypoglycemic lipid health benefits.The results revealed the regulation of AcMAT1-1-2 on the phenotype,sporulation and metabolism of sexual development,which provided a theoretical basis for better application of Golden-flower fungus fermentation in FBT.展开更多
基金National Natural Science Foundation of China(31860022)The Key Research and Development Program of Hunan Province(2024JK2150)。
文摘The economic value of Fuzhuan brick tea(FBT)is closely related to the sexual development of Golden-flower fungus,yet the regulatory mechanism of sexual development in the fungus remains unclear.This study identified a novel mating-type(MAT)gene AcMAT1-1-2 in Golden-flower fungus.The results showed that the deletion of AcMAT1-1-2 resulted in the minification of cleistothecium by 29.2%,the irregularity of colony morphology,the darkening of colony color,the highest number of ascomycetes and conidiophores unit area were each reduced by 16.6%;the interaction between AcMAT1-1-2 and AcMAT1-1-1 proteins;the AcMAT1-1-2 gene interacts with other related pathways by regulating the Biosynthesis of secondary metabolites pathway,which in turn regulates the mutant strains of AcMAT1-1-2 emerged new metabolites;the main differential metabolites have pyridines and derivatives,isoquinolines and derivatives,diazines,which may contribute to the formation of FBT quality and flavor,as well as anti-inflammatory,antioxidant and hypoglycemic lipid health benefits.The results revealed the regulation of AcMAT1-1-2 on the phenotype,sporulation and metabolism of sexual development,which provided a theoretical basis for better application of Golden-flower fungus fermentation in FBT.
