Nematodes play an important role in ecosystems;however,very little is known about their assembly processes and the factors influencing them.We studied nematode communities in bulk soils from three Asian mountain ecosy...Nematodes play an important role in ecosystems;however,very little is known about their assembly processes and the factors influencing them.We studied nematode communities in bulk soils from three Asian mountain ecosystems to determine the assembly processes of free-living nematode metacommunities and their driving factors.On each mountain,elevations span a range of climatic conditions with the potential to reveal assembly processes that predominate across multiple biomes.A phylogenetic null modeling framework was used to analyze 18S rRNA gene amplicons to quantify various assembly processes.We found that phylogenetic turnover between nematode communities on all mountains was dominated by stochastic processes,with“undominated processes”being the most predominant stochastic factor.Elevation has a significant impact on the relative importance of deterministic and stochastic processes.A variety of climatic and edaphic variables significantly influenced the variations in community assembly processes with elevation,even though their impacts were not consistent between the mountains.Overall,our results indicate that free-living nematode metacommunities in a wide range of environments are largely structured by stochastic processes rather than by niche-based deterministic processes,suggesting that metacommunities of soil free-living nematodes may respond to climate change in a largely unpredictable way.展开更多
Fungi outperformed bacterial in maintaining the microbial co-occurrence networks.Fungi showed different elevational network co-occurrence pattern from bacteria.Distinct biotic/abiotic factors influenced bacterial and ...Fungi outperformed bacterial in maintaining the microbial co-occurrence networks.Fungi showed different elevational network co-occurrence pattern from bacteria.Distinct biotic/abiotic factors influenced bacterial and fungal network dynamics.The interplay between soil micro-organisms in mountain ecosystems critically influences soil biogeochemical cycles and ecosystem processes.However,factors affecting the co-occurrence patterns of soil microbial communities remain unclear.In an attempt to understand how these patterns shift with elevation and identify the key explanatory factors underpinning these changes,we studied soil bacterial and fungal co-occurrence networks on Mt.Seorak,Republic of Korea.Amplicon sequencing was used to target the 16S rRNA gene and ITS2 region for bacteria and fungi,respectively.In contrast to bacteria,we found that fungi were predominantly situated in the core positions of the network,with significantly weakened co-occurrence with increasing elevation.The different co-occurrence patterns of fungal and bacterial communities could be resulted from their distinct responses to various environments.Both abiotic and biotic factors contributed significantly to shaping co-occurrence networks of bacterial and fungal communities.Fungal richness,bacterial community composition(indicated by PCoA1),and soil pH were the predominant factors influencing the variation in the entire microbial co-occurrence network.Biotic factors,such as the composition and diversity of bacterial communities,significantly influenced bacterial co-occurrence networks.External biotic and abiotic factors,including climatic and vegetative conditions,had a significant influence on fungal co-occurrence networks.These findings enhance our understanding of soil microbiota co-occurrences and deepen our knowledge of soil microbiota responses to climatic changes across elevational gradients in mountain ecosystems.展开更多
基金supported by the National Research Foundation of Korea(NRF)grant funded by the Korean government(No.NRF-2018R1C1B6007755)supported by a grant(No.20SCIPC158976-01)from the Construction Technology Research Program funded by the Ministry of Land,Infrastructure,and Transport of the Korean government+2 种基金the Natural Science Foundation of Guangxi,China(No.2018GXNSFDA 281006)the National Natural Science Foundation of China(No.41966005)the One Hundred Talents Project of Guangxi,China(No.6020303891251)。
文摘Nematodes play an important role in ecosystems;however,very little is known about their assembly processes and the factors influencing them.We studied nematode communities in bulk soils from three Asian mountain ecosystems to determine the assembly processes of free-living nematode metacommunities and their driving factors.On each mountain,elevations span a range of climatic conditions with the potential to reveal assembly processes that predominate across multiple biomes.A phylogenetic null modeling framework was used to analyze 18S rRNA gene amplicons to quantify various assembly processes.We found that phylogenetic turnover between nematode communities on all mountains was dominated by stochastic processes,with“undominated processes”being the most predominant stochastic factor.Elevation has a significant impact on the relative importance of deterministic and stochastic processes.A variety of climatic and edaphic variables significantly influenced the variations in community assembly processes with elevation,even though their impacts were not consistent between the mountains.Overall,our results indicate that free-living nematode metacommunities in a wide range of environments are largely structured by stochastic processes rather than by niche-based deterministic processes,suggesting that metacommunities of soil free-living nematodes may respond to climate change in a largely unpredictable way.
基金supported by the National Research Foundation of Korea(NRF)grant funded by the Korea Government(MSIP)(Grant Nos.NRF-2018R1C1B6007755 and NRF-2022R1F1A1066643)supported by a grant(No.20SCIP-C158976-01)from Construction Technology Research Program funded by Ministry of Land,Infrastructure and Transport of Korean Government+2 种基金the Guangxi Natural Science Foundation(Grant No.2018GXNSFDA281006)the National Natural Science Foundation of China(Grant No.41966005)the‘One Hundred Talents’Project of Guangxi(Grant No.6020303891251).
文摘Fungi outperformed bacterial in maintaining the microbial co-occurrence networks.Fungi showed different elevational network co-occurrence pattern from bacteria.Distinct biotic/abiotic factors influenced bacterial and fungal network dynamics.The interplay between soil micro-organisms in mountain ecosystems critically influences soil biogeochemical cycles and ecosystem processes.However,factors affecting the co-occurrence patterns of soil microbial communities remain unclear.In an attempt to understand how these patterns shift with elevation and identify the key explanatory factors underpinning these changes,we studied soil bacterial and fungal co-occurrence networks on Mt.Seorak,Republic of Korea.Amplicon sequencing was used to target the 16S rRNA gene and ITS2 region for bacteria and fungi,respectively.In contrast to bacteria,we found that fungi were predominantly situated in the core positions of the network,with significantly weakened co-occurrence with increasing elevation.The different co-occurrence patterns of fungal and bacterial communities could be resulted from their distinct responses to various environments.Both abiotic and biotic factors contributed significantly to shaping co-occurrence networks of bacterial and fungal communities.Fungal richness,bacterial community composition(indicated by PCoA1),and soil pH were the predominant factors influencing the variation in the entire microbial co-occurrence network.Biotic factors,such as the composition and diversity of bacterial communities,significantly influenced bacterial co-occurrence networks.External biotic and abiotic factors,including climatic and vegetative conditions,had a significant influence on fungal co-occurrence networks.These findings enhance our understanding of soil microbiota co-occurrences and deepen our knowledge of soil microbiota responses to climatic changes across elevational gradients in mountain ecosystems.
