AIM:To globally compare the gene expression profiles during the capillary morphogenesis of human microvascular endothelial cells (HMVECs) in an in vitro angiogeness system with affymetrix oligonucleotide array. METHOD...AIM:To globally compare the gene expression profiles during the capillary morphogenesis of human microvascular endothelial cells (HMVECs) in an in vitro angiogeness system with affymetrix oligonucleotide array. METHODS: A microcarrier-based in vitro angiogenesis system was developed, in which ECs migrated into the matrix, proliferated, and formed capillary sprouts. The sprouts elongated, branched and formed networks. The total RNA samples from the HMVECs at the selected time points (0.5, 24, and 72 h) during the capillary morphogenesis were used for microarray analyses, and the data were processed with the softwares provided by the manufacturers. The expression patterns of some genes were validated and confirmed by semi-quantitative RT-PCR. The regulated genes were grouped based on their molecular functions and expression patterns, and among them the expression of chemokines and chemokine receptors was specially examined and their functional implications were analyzed. RESULTS: A total of 1 961 genes were up- or downreg-ulated two-folds or above, and among them, 468 genes were up- or down-regulated three-folds or above. The regulated genes could be grouped into categories based on their molecular functions, and were also clustered into six groups based on their patterns of expression. As for chemokines and chemokine receptors, CXCL1/GRO-α, CXCL2/GRO-β, CXCL5/ENA-78, CXCL6/GCP2, IL-8/CXCL8, CXCL12/SDF-1, CXCL9/Mig, CXC11/ITAC, OOCL1/fractalkine, CCL2/MCP-1, CCL3, CCL5/RANTES, CCL7, CCL15, CCL21, CCL23, CCL28, and CCR1, CCR9, CXCR4 were identified. Moreover, these genes demonstrated different changing patterns during the capillary morphogenesis, which implied that they might have different roles in the sequential process. Among the chemokines identified, CCL2/MCP-1, CCL5/RANTES and CX3CL1 were specially up-regulated at the 24-h time point when the sprouting characterized the morphological change. It was thus suggested that they might exert crucial roles at the early stage of angiogenesis. CONCLUSION: The present study demonstrates a global profile of gene expression during endothelial capillary morphogenesis, and the results provide us much information about the molecular mechanisms of angiogenesis, with which further evaluation of individual genes can be conducted.展开更多
Objective: to analyze the effect of continuous quality improvement intervention for patients with surgical catheter receiving general surgical catheter care. Methods: a total of 990 patients with surgical catheterizat...Objective: to analyze the effect of continuous quality improvement intervention for patients with surgical catheter receiving general surgical catheter care. Methods: a total of 990 patients with surgical catheterization admitted to our hospital from March 2021 to March 2022 were selected and randomly divided into two groups, the patients who received conventional general surgical pipeline nursing program (n 495), that is, the control group, and the patients who received continuous quality improvement intervention (n 495), that is, the observation group. The intervention effects of the two groups were compared. Results: the incidence of adverse events, SAS and SDS scores of observation group were lower than those of control group;the nursing satisfaction of observation group was higher than that of control group;the number of qualified cases in theory and skills of nursing staff in observation group was higher than that of control group, and the qualified scores in theory and skills were higher than that of control group;the difference was statistically significant (P < 0.05). Conclusion: patients with clinical surgical catheterization are faced with great treatment risks in the process of treatment, including unplanned extubation, which will not only have a great impact on patients normal treatment work, but also interfere with patients normal emotional state, with high risk. Combining continuous quality improvement measures to nurse the pipeline can standardize the catheter technology and operation steps, reduce the probability of risk events to the lowest possible level and thus improve the level of nursing quality, which is worthy of clinical application.展开更多
文摘AIM:To globally compare the gene expression profiles during the capillary morphogenesis of human microvascular endothelial cells (HMVECs) in an in vitro angiogeness system with affymetrix oligonucleotide array. METHODS: A microcarrier-based in vitro angiogenesis system was developed, in which ECs migrated into the matrix, proliferated, and formed capillary sprouts. The sprouts elongated, branched and formed networks. The total RNA samples from the HMVECs at the selected time points (0.5, 24, and 72 h) during the capillary morphogenesis were used for microarray analyses, and the data were processed with the softwares provided by the manufacturers. The expression patterns of some genes were validated and confirmed by semi-quantitative RT-PCR. The regulated genes were grouped based on their molecular functions and expression patterns, and among them the expression of chemokines and chemokine receptors was specially examined and their functional implications were analyzed. RESULTS: A total of 1 961 genes were up- or downreg-ulated two-folds or above, and among them, 468 genes were up- or down-regulated three-folds or above. The regulated genes could be grouped into categories based on their molecular functions, and were also clustered into six groups based on their patterns of expression. As for chemokines and chemokine receptors, CXCL1/GRO-α, CXCL2/GRO-β, CXCL5/ENA-78, CXCL6/GCP2, IL-8/CXCL8, CXCL12/SDF-1, CXCL9/Mig, CXC11/ITAC, OOCL1/fractalkine, CCL2/MCP-1, CCL3, CCL5/RANTES, CCL7, CCL15, CCL21, CCL23, CCL28, and CCR1, CCR9, CXCR4 were identified. Moreover, these genes demonstrated different changing patterns during the capillary morphogenesis, which implied that they might have different roles in the sequential process. Among the chemokines identified, CCL2/MCP-1, CCL5/RANTES and CX3CL1 were specially up-regulated at the 24-h time point when the sprouting characterized the morphological change. It was thus suggested that they might exert crucial roles at the early stage of angiogenesis. CONCLUSION: The present study demonstrates a global profile of gene expression during endothelial capillary morphogenesis, and the results provide us much information about the molecular mechanisms of angiogenesis, with which further evaluation of individual genes can be conducted.
文摘Objective: to analyze the effect of continuous quality improvement intervention for patients with surgical catheter receiving general surgical catheter care. Methods: a total of 990 patients with surgical catheterization admitted to our hospital from March 2021 to March 2022 were selected and randomly divided into two groups, the patients who received conventional general surgical pipeline nursing program (n 495), that is, the control group, and the patients who received continuous quality improvement intervention (n 495), that is, the observation group. The intervention effects of the two groups were compared. Results: the incidence of adverse events, SAS and SDS scores of observation group were lower than those of control group;the nursing satisfaction of observation group was higher than that of control group;the number of qualified cases in theory and skills of nursing staff in observation group was higher than that of control group, and the qualified scores in theory and skills were higher than that of control group;the difference was statistically significant (P < 0.05). Conclusion: patients with clinical surgical catheterization are faced with great treatment risks in the process of treatment, including unplanned extubation, which will not only have a great impact on patients normal treatment work, but also interfere with patients normal emotional state, with high risk. Combining continuous quality improvement measures to nurse the pipeline can standardize the catheter technology and operation steps, reduce the probability of risk events to the lowest possible level and thus improve the level of nursing quality, which is worthy of clinical application.
