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荧光探针偶联GPC3单链抗体对肝癌的体内外检测研究 被引量:1
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作者 杜恩辅 徐霖 +5 位作者 周选民 刘梅讯 张晓龙 崔宁 侯平志 余惠芬 《检验医学与临床》 CAS 2018年第11期1583-1586,共4页
目的使用单链抗体偶联荧光染料构建探针进行磷脂酰肌醇蛋白聚糖3(GPC3)阳性肝癌的体内外检测。方法通过Overlap聚合酶链反应(PCR)采用柔性肽G4S将罗氏公司开发的Codrituzumab轻链与重链连接,构建工程载体,并通过毕赤酵母进行表达纯化,... 目的使用单链抗体偶联荧光染料构建探针进行磷脂酰肌醇蛋白聚糖3(GPC3)阳性肝癌的体内外检测。方法通过Overlap聚合酶链反应(PCR)采用柔性肽G4S将罗氏公司开发的Codrituzumab轻链与重链连接,构建工程载体,并通过毕赤酵母进行表达纯化,获得靶向GPC3的单链抗体;采用流式细胞术检测单链抗体对GPC3阳性肝癌细胞Huh-7的结合能力;将单链抗体与碱性荧光染料罗丹明B偶联,使用激光共聚焦检测单链抗体在体外对Huh-7细胞的靶向能力;将单链抗体与近红外荧光染料NIRB-NHS偶联,通过CCD照相机实时收集荧光信号检测偶联探针在荷瘤裸鼠体内的靶向能力,并判断其开发为检测试剂的潜力。结果通过基因工程手段和毕赤酵母表达,成功获得抗GPC3单链抗体,经WB鉴定结果说明单链抗体AntiGPC3scFv表达及装配正确。流式细胞术检测Anti-GPC3scFv与肝癌细胞Huh-7的结合率为40.3%。体外靶向性试验验证单链抗体Anti-GPC3scFv可在体外很好地靶向肝癌细胞Huh-7,单链抗体组细胞平均荧光强度为218.61±9.03,明显优于对照组的31.74±5.28。体内近红外成像试验中,通过Huh-7细胞移植荷瘤裸鼠给药后的荧光信号分析结果显示,单链抗体探针可以很好地靶向至肿瘤部位,分析热点区域的最大肿瘤/正常组织荧光信号比可知,单链抗体组信号比为8.27±2.94,明显强于对照组的1.09±0.63。结论采用毕赤酵母表达体系成功构建并表达了单链抗体Anti-GPC3scFv。单链抗体保留了母本抗体的结合能力,并体现了很好的体外和体内靶向能力,其靶向能力可应用于GPC3阳性肝癌的检测领域,拥有潜在的临床应用前景。 展开更多
关键词 磷脂酰肌醇蛋白聚糖3 荧光探针 肝癌 单链抗体 激光共聚焦 近红外成像
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Effect of Comprehensive Nursing on the Quality of Life of Patients with Bladder Tumor Undergoing Urostomy
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作者 cuining 《外文科技期刊数据库(文摘版)医药卫生》 2022年第4期113-117,共5页
Objective: to explore the effect of comprehensive nursing in improving the quality of life of patients with bladder tumor surgery (urostomy). Methods: the samples of this study were 80 patients with bladder tumor surg... Objective: to explore the effect of comprehensive nursing in improving the quality of life of patients with bladder tumor surgery (urostomy). Methods: the samples of this study were 80 patients with bladder tumor surgery (concurrent urostomy) in our hospital. The operation time in our hospital was from May 2019 to May 2021. The samples were divided into 40 cases of reference group and 40 cases by computer table method. The research group was given basic nursing, comprehensive nursing + basic nursing, and the improvement of quality of life, compliance during nursing, and nursing satisfaction of the two groups of samples were required. Results: compared with the reference group, the emotional, physical and other quality of life scores of the research group were higher after intervention, P < 0.05, and the difference was larger;the sample symptom score of the reference group was higher after intervention, P < 0.05, the difference was larger than that of the reference group. The sample compliance and nursing satisfaction of the reference group were lower, P < 0.05, the difference was large. Conclusion: the application of comprehensive nursing in patients undergoing urostomy for bladder tumor surgery is very effective, which can improve their physical, emotional and other functions, and at the same time help to improve the compliance of patients during treatment, and patients prefer this nursing content, which is worthy of wide clinical application. 展开更多
关键词 urostomy psychological intervention comprehensive nursing bladder tumor quality of life DYSURIA
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Application of Comfort Nursing in Severe Acute Pancreatitis
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作者 XIANGZijun cuining 《外文科技期刊数据库(文摘版)医药卫生》 2022年第3期039-042,共4页
Objective: this article is mainly to explore the practical role of providing comfort care for patients with acute pancreatitis during gastrointestinal decompression, so as to explore the ways to promote the effective ... Objective: this article is mainly to explore the practical role of providing comfort care for patients with acute pancreatitis during gastrointestinal decompression, so as to explore the ways to promote the effective recovery of patients. Methods: patients with acute pancreatitis were divided into two groups: 31 people as a control group and 31 people as an experimental group. Our care of the control group still treats the patients according to the usual hospital care, while in the experimental group, we will adopt the comfort care of our experiment while performing the usual care of the patients. Results: there were significant differences between patients in control and experimental groups during gastrointestinal decompression. Conclusions: our comfortable care of patients with acute pancreatitis during their gastrointestinal decompression can effectively reduce the discomfort caused during their gastrointestinal decompression, thus improving patient satisfaction with hospital care services. 展开更多
关键词 comfortable care acute pancreatitis gastrointestinal decompression
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