As abscisic acid(ABA)receptor,the pyrabactin resistance 1-like(PYR/PYL)protein(named PYL for simplicity)plays an important part to unveil the signal transduction of ABA and its regulatory mechanisms.Glycyrrhiza uralen...As abscisic acid(ABA)receptor,the pyrabactin resistance 1-like(PYR/PYL)protein(named PYL for simplicity)plays an important part to unveil the signal transduction of ABA and its regulatory mechanisms.Glycyrrhiza uralensis,a drought-tolerant medicinal plant,is a good model for the mechanism analysis of ABA response and active compound biosynthesis.However,knowledge about PYL family in G.uralensis remains largely unknown.Here,10 PYLs were identified in G.uralensis genome.Characterization analysis indicated that PYLs in G.uralensis(Gu PYLs)are relatively conserved.Phylogenetic analysis showed that Gu PYL1-3 belongs to subfamily I,Gu PYL4-6 and Gu PYL10 belong to subfamily II and Gu PYL7-9 belongs to subfamily III.In addition,transcriptome data presented various expression levels of Gu PYLs under different exogenous ABA stresses.The expression pattern of Gu PYLs was verified by Quantitative real-time polymerase chain reaction(q RT-PCR).The study proved that Gu PYL4,Gu PYL5,Gu PYL8 and Gu PYL9 genes are significantly up-regulated by ABA stress and the response process is dynamic.This study paves the way for elucidating the regulation mechanism of ABA signal to secondary metabolites and improving the cultivation and quality of G.uralensis using agricultural strategies.展开更多
Pyrrosia petiolosa,Pyrrosia lingua and Pyrrosia sheareri are recorded as original plants of Pyrrosiae Folium(PF)and commonly used as Chinese herbal medicines.Due to the similar morphological features of PF and its adu...Pyrrosia petiolosa,Pyrrosia lingua and Pyrrosia sheareri are recorded as original plants of Pyrrosiae Folium(PF)and commonly used as Chinese herbal medicines.Due to the similar morphological features of PF and its adulterants,common DNA barcodes cannot accurately distinguish PF species.Knowledge of the chloroplast(cp)genome is widely used in species identification,molecular marker and phylogenetic analyses.Herein,we determined the complete cp genomes of three original species of PF via highthroughput sequencing technologies.The three cp genomes exhibited a typical quadripartite structure with sizes ranging from 158165 to 163026 bp.The cp genomes of P.petiolosa and P.lingua encoded 130 genes,whilst that of P.sheareri encoded 131 genes.The complete cp genomes were compared,and five highly divergent regions of pet A-psb J,mat K-rps16,ndh C-trn M,psb M-pet N and psa Cndh E were screened as potential DNA barcodes for identification of Pyrrosia genus species.The phylogenetic tree we obtained indicated that P.petiolosa and P.lingua are clustered in a single clade and,thus,share a close relationship.This study provides invaluable information for further studies on the species identification,taxonomy and phylogeny of Pyrrosia genus species.展开更多
In order to deepen our rational understanding of Chinese characters,it is necessary to reexamine the value of it today.The reason why Chinese characters can keep vital is that it fits the unique thinking mode and aest...In order to deepen our rational understanding of Chinese characters,it is necessary to reexamine the value of it today.The reason why Chinese characters can keep vital is that it fits the unique thinking mode and aesthetic standards of the Han People.In the meantime,it intervenes in Chinese ontology by improving the resolution ratio of information trans,and objectively performs the function of the fourth element of language.展开更多
基金supported by the National Science and Technology Major Project for“Significant New Drugs Development”(No.2019ZX09201005-006-003)the ChinesAcademy of Medical Sciences(CAMS)Innovation Fund for Medical Sciences(CIFMS)(No.2016-I2M-3-016)。
文摘As abscisic acid(ABA)receptor,the pyrabactin resistance 1-like(PYR/PYL)protein(named PYL for simplicity)plays an important part to unveil the signal transduction of ABA and its regulatory mechanisms.Glycyrrhiza uralensis,a drought-tolerant medicinal plant,is a good model for the mechanism analysis of ABA response and active compound biosynthesis.However,knowledge about PYL family in G.uralensis remains largely unknown.Here,10 PYLs were identified in G.uralensis genome.Characterization analysis indicated that PYLs in G.uralensis(Gu PYLs)are relatively conserved.Phylogenetic analysis showed that Gu PYL1-3 belongs to subfamily I,Gu PYL4-6 and Gu PYL10 belong to subfamily II and Gu PYL7-9 belongs to subfamily III.In addition,transcriptome data presented various expression levels of Gu PYLs under different exogenous ABA stresses.The expression pattern of Gu PYLs was verified by Quantitative real-time polymerase chain reaction(q RT-PCR).The study proved that Gu PYL4,Gu PYL5,Gu PYL8 and Gu PYL9 genes are significantly up-regulated by ABA stress and the response process is dynamic.This study paves the way for elucidating the regulation mechanism of ABA signal to secondary metabolites and improving the cultivation and quality of G.uralensis using agricultural strategies.
基金supported by the Major Scientific and Technological Special Project for“Significant New Drugs Creation”(No.2018ZX09711001-008-007)the Chinese Academy of Medical Sciences(CAMS)Innovation Fund for Medical Sciences(CIFMS,No.2016-I2M-3-016)。
文摘Pyrrosia petiolosa,Pyrrosia lingua and Pyrrosia sheareri are recorded as original plants of Pyrrosiae Folium(PF)and commonly used as Chinese herbal medicines.Due to the similar morphological features of PF and its adulterants,common DNA barcodes cannot accurately distinguish PF species.Knowledge of the chloroplast(cp)genome is widely used in species identification,molecular marker and phylogenetic analyses.Herein,we determined the complete cp genomes of three original species of PF via highthroughput sequencing technologies.The three cp genomes exhibited a typical quadripartite structure with sizes ranging from 158165 to 163026 bp.The cp genomes of P.petiolosa and P.lingua encoded 130 genes,whilst that of P.sheareri encoded 131 genes.The complete cp genomes were compared,and five highly divergent regions of pet A-psb J,mat K-rps16,ndh C-trn M,psb M-pet N and psa Cndh E were screened as potential DNA barcodes for identification of Pyrrosia genus species.The phylogenetic tree we obtained indicated that P.petiolosa and P.lingua are clustered in a single clade and,thus,share a close relationship.This study provides invaluable information for further studies on the species identification,taxonomy and phylogeny of Pyrrosia genus species.
文摘In order to deepen our rational understanding of Chinese characters,it is necessary to reexamine the value of it today.The reason why Chinese characters can keep vital is that it fits the unique thinking mode and aesthetic standards of the Han People.In the meantime,it intervenes in Chinese ontology by improving the resolution ratio of information trans,and objectively performs the function of the fourth element of language.
