[目的]探讨改良小切口股骨近端防旋髓内钉(proximal femoral nail anti-rotation,PFNA)治疗股骨转子间骨折的临床疗效。[方法] 2019年1月—2021年1月对84例股骨转子间骨折采用PFNA术治疗,依据术前医患沟通结果,44例采用改良小切口PFNA术...[目的]探讨改良小切口股骨近端防旋髓内钉(proximal femoral nail anti-rotation,PFNA)治疗股骨转子间骨折的临床疗效。[方法] 2019年1月—2021年1月对84例股骨转子间骨折采用PFNA术治疗,依据术前医患沟通结果,44例采用改良小切口PFNA术(小切口组),40例采用常规切口PFNA术(常规组),比较围手术期资料以及随访结果。[结果]两组均顺利完成手术,无严重术中并发症。小切口组切口总长度[(5.3±1.3) cm vs (7.0±1.4) cm,P<0.001]、术中出血量[(88.3±19.2) ml vs(106.3±22.4) ml,P<0.001]、透视次数[(12.0±2.5)次vs (15.2±3.2)次,P<0.001]均显著优于常规组。但是,两组间手术时间、下地行走时间、住院时间、切口愈合等级的差异无统计学意义(P>0.05)。两组均随访12个月以上,两组恢复完全负重时间的差异无统计学意义(P>0.05)。随着时间推移,两组VAS评分逐渐减少,Harris评分、髋伸屈ROM、髋内-外旋ROM逐渐增加(P<0.05)。术后相应时间点两组间VAS评分、Harris评分、髋伸屈ROM、髋内-外旋ROM的差异均无统计学意义(P>0.05)。影像方面,两组骨折复位质量、骨折愈合时间差异无统计学意义(P>0.05),两组术后即刻、末次随访颈干角均显著大于术前(P<0.05),相应时间点,两组间颈干角的差异均无统计学意义(P>0.05)。至末次随访时两组患者骨折均愈合,无内固定断裂、移位等不良影像改变。[结论]改良小切口PFNA术治疗股骨转子间骨折能减少术中创伤,临床效果与常规切口相当。展开更多
[ Objective] To investigate the effects of Newcastle disease vaccination and lead exposure on growth and development of egg yellow feather quail. [ Method] Ninety 14-day-old quails were assigned randomly to 10 groups ...[ Objective] To investigate the effects of Newcastle disease vaccination and lead exposure on growth and development of egg yellow feather quail. [ Method] Ninety 14-day-old quails were assigned randomly to 10 groups （ n = 9）, and they fed and drunk at liberty. A 2 ×5 factorial design was used. The quail immunized with ND vaccine and those non-immunized were exposed to lead at a dosage of 0, 50,500, 1 000 and 2 000 mg/L, respectively. [Result] The ND immunization reduced body weight significantly （ P 〈 0.01 ） and increased liver index and serum levels of hy- aluronic acid in quail within 1 -2 weeks post inoculation. The quail exposed to 2 000 mg/L lead acetate had significantly lower body weight than the control （ P 〈 0.01 ）. From 7 weeks old, the same inhibitory effect was observed in the quail＇exposed to lead at different dosages （ P 〈 0.01 ）. In addition, the lead poisoning could cause decreased feed intake, depilation, gonadal dysgenesis, and increased serum levels of hyaluronic acid. [ Conduslon] The inhibitory effect of ND vaccination on growth of quail occurs within 1 -2 weeks post inoculation; thus, the effects of immune stress should not be worried in quail production. The lead poisoning has significant effects on body weight and feed intake of quail, and lead dosage and duration affect the inhibitory effects of lead. Gonadal dysgenesis is an important indicator of lead poisoning in. quail. The serum levels of hyaluronic acid can be used to determine whether the quail have been immunized with ND vaccine or have contacted lead pollutants.展开更多
Alfalfa is widely grown and is one of the most important forage crops in the world, but its growth and biomass production are markedly reduced under salt and drought stress, particularly during the early seedling stag...Alfalfa is widely grown and is one of the most important forage crops in the world, but its growth and biomass production are markedly reduced under salt and drought stress, particularly during the early seedling stage. To identify the mechanisms behind salt and drought responsiveness at the alfalfa seedling stage, the proteins expressed were analyzed under no-treatment, 200 mol L–1 Na Cl and 180 g L–1 PEG treatment conditions during the seedling stage. Out of more than 800 protein spots detected on two-dimensional electrophoresis（2-DE） g els, 35 proteins showed statistically significant responses（P〈0.05） to Na Cl and PEG stress, which were selected for tandem mass spectrometric identification, owing to their good resolution and abundance levels, and 32 proteins were positively identified. The identified proteins were divided into seven functional categories： photosynthetic metabolism, protein biosynthesis, folding and assembly, carbohydrate metabolism-associated proteins, stress defense related protein, metabolism of nucleic acid, other function categories and unknown proteins. Our results suggested that these proteins may play roles in alfalfa adaptation to salt and drought stress. Further study of these proteins will provide insights into the molecular mechanisms of abiotic stress and the discovery of new candidate markers in alfalfa.展开更多
文摘[目的]探讨改良小切口股骨近端防旋髓内钉(proximal femoral nail anti-rotation,PFNA)治疗股骨转子间骨折的临床疗效。[方法] 2019年1月—2021年1月对84例股骨转子间骨折采用PFNA术治疗,依据术前医患沟通结果,44例采用改良小切口PFNA术(小切口组),40例采用常规切口PFNA术(常规组),比较围手术期资料以及随访结果。[结果]两组均顺利完成手术,无严重术中并发症。小切口组切口总长度[(5.3±1.3) cm vs (7.0±1.4) cm,P<0.001]、术中出血量[(88.3±19.2) ml vs(106.3±22.4) ml,P<0.001]、透视次数[(12.0±2.5)次vs (15.2±3.2)次,P<0.001]均显著优于常规组。但是,两组间手术时间、下地行走时间、住院时间、切口愈合等级的差异无统计学意义(P>0.05)。两组均随访12个月以上,两组恢复完全负重时间的差异无统计学意义(P>0.05)。随着时间推移,两组VAS评分逐渐减少,Harris评分、髋伸屈ROM、髋内-外旋ROM逐渐增加(P<0.05)。术后相应时间点两组间VAS评分、Harris评分、髋伸屈ROM、髋内-外旋ROM的差异均无统计学意义(P>0.05)。影像方面,两组骨折复位质量、骨折愈合时间差异无统计学意义(P>0.05),两组术后即刻、末次随访颈干角均显著大于术前(P<0.05),相应时间点,两组间颈干角的差异均无统计学意义(P>0.05)。至末次随访时两组患者骨折均愈合,无内固定断裂、移位等不良影像改变。[结论]改良小切口PFNA术治疗股骨转子间骨折能减少术中创伤,临床效果与常规切口相当。
基金funded by the Key Scientific and Technological Project of Henan Province ( 082102130002)
文摘[ Objective] To investigate the effects of Newcastle disease vaccination and lead exposure on growth and development of egg yellow feather quail. [ Method] Ninety 14-day-old quails were assigned randomly to 10 groups （ n = 9）, and they fed and drunk at liberty. A 2 ×5 factorial design was used. The quail immunized with ND vaccine and those non-immunized were exposed to lead at a dosage of 0, 50,500, 1 000 and 2 000 mg/L, respectively. [Result] The ND immunization reduced body weight significantly （ P 〈 0.01 ） and increased liver index and serum levels of hy- aluronic acid in quail within 1 -2 weeks post inoculation. The quail exposed to 2 000 mg/L lead acetate had significantly lower body weight than the control （ P 〈 0.01 ）. From 7 weeks old, the same inhibitory effect was observed in the quail＇exposed to lead at different dosages （ P 〈 0.01 ）. In addition, the lead poisoning could cause decreased feed intake, depilation, gonadal dysgenesis, and increased serum levels of hyaluronic acid. [ Conduslon] The inhibitory effect of ND vaccination on growth of quail occurs within 1 -2 weeks post inoculation; thus, the effects of immune stress should not be worried in quail production. The lead poisoning has significant effects on body weight and feed intake of quail, and lead dosage and duration affect the inhibitory effects of lead. Gonadal dysgenesis is an important indicator of lead poisoning in. quail. The serum levels of hyaluronic acid can be used to determine whether the quail have been immunized with ND vaccine or have contacted lead pollutants.
基金supported by the National Key Basic Research Program of China (2014CB138703-2)the National Natural Science Foundation of China (31472139)the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences (ASTIPIAS14)
文摘Alfalfa is widely grown and is one of the most important forage crops in the world, but its growth and biomass production are markedly reduced under salt and drought stress, particularly during the early seedling stage. To identify the mechanisms behind salt and drought responsiveness at the alfalfa seedling stage, the proteins expressed were analyzed under no-treatment, 200 mol L–1 Na Cl and 180 g L–1 PEG treatment conditions during the seedling stage. Out of more than 800 protein spots detected on two-dimensional electrophoresis（2-DE） g els, 35 proteins showed statistically significant responses（P〈0.05） to Na Cl and PEG stress, which were selected for tandem mass spectrometric identification, owing to their good resolution and abundance levels, and 32 proteins were positively identified. The identified proteins were divided into seven functional categories： photosynthetic metabolism, protein biosynthesis, folding and assembly, carbohydrate metabolism-associated proteins, stress defense related protein, metabolism of nucleic acid, other function categories and unknown proteins. Our results suggested that these proteins may play roles in alfalfa adaptation to salt and drought stress. Further study of these proteins will provide insights into the molecular mechanisms of abiotic stress and the discovery of new candidate markers in alfalfa.
