The 99mTc-3PRGD2 targeted SPECT/CT scanning was of significance in detecting differentiated glioma.In this work,the diagnostic value of 99mTc-3PRGD2 scintigraphy in brain glioma was evaluated by the ten clinically ver...The 99mTc-3PRGD2 targeted SPECT/CT scanning was of significance in detecting differentiated glioma.In this work,the diagnostic value of 99mTc-3PRGD2 scintigraphy in brain glioma was evaluated by the ten clinically verified brain glioma patients after obtaining informed consent.The patients first accepted X-ray imaging to localize the detecting regions before administrating with 99mTc-3PRGD2 at a mean radioactivity of 849±115 MBq via single intravenous bolus injection 2 h prior to SPECT/CT imaging.Tumor samples for detectingαvβ3 were collected by surgical operations two weeks after the scintigraphy.The results of CT and SPECT scanning were merged and compared.The correlation between tumor occupation（T/N ratio） andαvβ3 expression level were analyzed.The T/N ratios in brain glioma were proportionally correlated to av(33 positive cell percentage（R2=0.9253,p<0.05）.This study primarily evaluated the clinical application of 99mTc-3PRGD2 SPECT scintigraphy on brain glioma.The more pathological types and detecting strategies covering a large amount of samples would aid to clarify the potentials.展开更多
To evaluate safety and therapeutic efficacy of sodium glycididazole(CMNa)combined with 131I radiotherapy for differentiated thyroid carcinoma(DTC),the 60 patients of DTC therapeutic protocols were selected and divided...To evaluate safety and therapeutic efficacy of sodium glycididazole(CMNa)combined with 131I radiotherapy for differentiated thyroid carcinoma(DTC),the 60 patients of DTC therapeutic protocols were selected and divided into 3 groups of the DTC 4.44 GBq 131I,DTC 3.70 GBq 131I,and combination of DTC 3.70 GBq 131I with CMNa,and the 20 patients of Graves'Disease were selected as the control group.Peripheral blood was sampled at 131I pre-treatment of 1 day,and 131I post-treatment of 7,91,and 182 days,thus analyzing lymphocyte micronucleus scores and karyotyping profiles.Compared with the control group,the lymphocyte micronucleus and chromosome mutation rates in 131I treated DTC increased after post-treatment of 7 days,recovered after post-treatment of 91 days,and did not bounce after post-treatment of 182 days.The micronucleus and chromosome mutation rates in the combination of DTC 3.70 GBq 131I with CMNa showed less significant variation than other treated DTC groups.Our results demonstrate that micronucleus assay and karyotyping analysis are favorable to evaluate 131I radiotherapy for DTC.The combination of the CMNa with 131I radiotherapy was safe for DTC patients without affecting the long-term therapeutic outcomes.展开更多
To evaluate the ^(125)I radioactive probing re-canalizing stenostic nasolacrimal duct,the nasolacrimal duct stenosis models in epithelium and connective tissues are experimentally structured by inbred white rabbits(Ne...To evaluate the ^(125)I radioactive probing re-canalizing stenostic nasolacrimal duct,the nasolacrimal duct stenosis models in epithelium and connective tissues are experimentally structured by inbred white rabbits(New Zealand),including the nasolacrimal duct stenosis,the mechanical probing with outer layer of thermoplastic tube,and the ^(125)I radioactive probing with the ^(125)I seeds sealing into the thermoplastic tube.After re-canalized for four weeks, tissue specimens from bilateral nasolacrimal ducts are obtained,and the Bcl-2 and Bax protein expression levels are evaluated by immunohistochemical staining analysis.Comparing with the blank control,the expression levels of the Bcl-2 and Bax in the nasolacrimal duct stenosis and the mechanical probing are significantly up-/down-regulated(p<0.05),but in the ^(125)I radioactive probing are down-/up-regulated(p<0.05) and can be used to re-canalize the stenostic lacrimal passage.The results show that the ^(125)I radioactive probing is a therapeutical mechanism for radioactive probing strategy for treating nasolacrimal duct stenosis to induce cell apoptosis.展开更多
基金Supported by the National Natural Science Foundation of China projects(No.81271606)the Research Fund of Science and Technology Department of Jilin province(No.200705327,No.201015185,and No.201201041)the Research Fund of Shenzhen Sci-tech Department of Guangdong province (No.201102154)
文摘The 99mTc-3PRGD2 targeted SPECT/CT scanning was of significance in detecting differentiated glioma.In this work,the diagnostic value of 99mTc-3PRGD2 scintigraphy in brain glioma was evaluated by the ten clinically verified brain glioma patients after obtaining informed consent.The patients first accepted X-ray imaging to localize the detecting regions before administrating with 99mTc-3PRGD2 at a mean radioactivity of 849±115 MBq via single intravenous bolus injection 2 h prior to SPECT/CT imaging.Tumor samples for detectingαvβ3 were collected by surgical operations two weeks after the scintigraphy.The results of CT and SPECT scanning were merged and compared.The correlation between tumor occupation（T/N ratio） andαvβ3 expression level were analyzed.The T/N ratios in brain glioma were proportionally correlated to av(33 positive cell percentage（R2=0.9253,p<0.05）.This study primarily evaluated the clinical application of 99mTc-3PRGD2 SPECT scintigraphy on brain glioma.The more pathological types and detecting strategies covering a large amount of samples would aid to clarify the potentials.
基金Supported by the Research Fund of Science and Technology Department of Jilin Province(no.201015185)
文摘To evaluate safety and therapeutic efficacy of sodium glycididazole(CMNa)combined with 131I radiotherapy for differentiated thyroid carcinoma(DTC),the 60 patients of DTC therapeutic protocols were selected and divided into 3 groups of the DTC 4.44 GBq 131I,DTC 3.70 GBq 131I,and combination of DTC 3.70 GBq 131I with CMNa,and the 20 patients of Graves'Disease were selected as the control group.Peripheral blood was sampled at 131I pre-treatment of 1 day,and 131I post-treatment of 7,91,and 182 days,thus analyzing lymphocyte micronucleus scores and karyotyping profiles.Compared with the control group,the lymphocyte micronucleus and chromosome mutation rates in 131I treated DTC increased after post-treatment of 7 days,recovered after post-treatment of 91 days,and did not bounce after post-treatment of 182 days.The micronucleus and chromosome mutation rates in the combination of DTC 3.70 GBq 131I with CMNa showed less significant variation than other treated DTC groups.Our results demonstrate that micronucleus assay and karyotyping analysis are favorable to evaluate 131I radiotherapy for DTC.The combination of the CMNa with 131I radiotherapy was safe for DTC patients without affecting the long-term therapeutic outcomes.
基金Supported by Natural Science Foundation of Jilin Province(No.200705327)Project of Science and Technology of Changchun City(2007GH25)
文摘To evaluate the ^(125)I radioactive probing re-canalizing stenostic nasolacrimal duct,the nasolacrimal duct stenosis models in epithelium and connective tissues are experimentally structured by inbred white rabbits(New Zealand),including the nasolacrimal duct stenosis,the mechanical probing with outer layer of thermoplastic tube,and the ^(125)I radioactive probing with the ^(125)I seeds sealing into the thermoplastic tube.After re-canalized for four weeks, tissue specimens from bilateral nasolacrimal ducts are obtained,and the Bcl-2 and Bax protein expression levels are evaluated by immunohistochemical staining analysis.Comparing with the blank control,the expression levels of the Bcl-2 and Bax in the nasolacrimal duct stenosis and the mechanical probing are significantly up-/down-regulated(p<0.05),but in the ^(125)I radioactive probing are down-/up-regulated(p<0.05) and can be used to re-canalize the stenostic lacrimal passage.The results show that the ^(125)I radioactive probing is a therapeutical mechanism for radioactive probing strategy for treating nasolacrimal duct stenosis to induce cell apoptosis.
