The growth performance,nutrient removal,lipid accumulation and morphological changes of Cyanobacterium aponinum OUC1 and Scenedesmus obliquus which were cultured in secondary effluents from two wastewater treatment pl...The growth performance,nutrient removal,lipid accumulation and morphological changes of Cyanobacterium aponinum OUC1 and Scenedesmus obliquus which were cultured in secondary effluents from two wastewater treatment plants:Tuandao Wastewater Treatment Plant(ETD)and Licun River Wastewater Treatment Plant(ELR)were investigated.The results showed that both C.aponinum OUC1 and S.obliquus have superior growth performances in both undiluted effluents,while the better of them was that in ETD effluent,with cell densities of C.aponinum OUC1 and S.obliquus increased by 159%and 66%over that of BG11(control),respectively.Regarding nutrient removal,S.obliquus could completely remove inorganic phosphorus,and decrease ammonia nitrogen in ETD effluent by 81%.In addition,both C.aponinum OUC1 and S.obliquus cultivated in ETD exhibited extraordinary potential for biofuel production,increasing lipid productivities by 133%and 89%of that cultivated in ELR,respectively.As to ultrastructural changes,the differences in the lipoidal globules and glycogen granules of S.obliquus and C.aponinum OUC1 among the ETD and ELR treatments were mostly related to phosphorus limitations.The findings from this research reveal the probability using the secondary effluents as cultivation media to enhance algal biomass,nutrient removal and lipid productivity.展开更多
This study determined growth and lipid accumulation in Nannochloropsis sp. MASCC 11 cultivated at different pH, temperatures, and CO2 concentrations in 10-day period. The suitability for biodiesel production was also ...This study determined growth and lipid accumulation in Nannochloropsis sp. MASCC 11 cultivated at different pH, temperatures, and CO2 concentrations in 10-day period. The suitability for biodiesel production was also evaluated based on the fatty acid profiles of microalgae lipid. Nannochloropsis sp. MASCC 11 showed an excellent tolerance to acidic pH(as low as 4.0), high temperatures(at least 40℃), and high CO2 concentrations(5%-15%), which are major stressed conditions in flue gas. The highest algal biomass was acquired at pH of 9.0(0.44 g L^-1), a temperature of 35℃(0.63 g L^-1), and a CO2 concentration of 5%(2.27 g L^-1). Maximum lipid production was obtained at p H of 6.0(108.2 mg L^-1), a temperature of 35℃(134.6 mg L^-1), and a CO2 concentration of 5%(782.7 mg L^-1). Synthesis of polyunsaturated fatty acids(PUFAs) in biomass was stimulated under high CO_2 concentrations, remaining above 80% of total fatty acids, mainly composed of C16:3, C18:2, and C18:3. This led to the algae-based biodiesel having a lower oxidation stability, better cold flow properties, and other parameters such as its kinematic viscosity, cetane number, and specific gravity complied with ASTM or EN 14214 biodiesel specifications. Therefore, the improvement of oxidative stability needs to be considered before Nannochloropsis sp. MASCC 11 lipid can be used for biodiesel production, even if this species can grow well under stressful conditions.展开更多
基金This work was supported by the National Marine Hazard Mitigation Service,Ministry of Natural Resources of the People’s Republic of China through its Commissioned Research Scheme(No.2019005AC).
文摘The growth performance,nutrient removal,lipid accumulation and morphological changes of Cyanobacterium aponinum OUC1 and Scenedesmus obliquus which were cultured in secondary effluents from two wastewater treatment plants:Tuandao Wastewater Treatment Plant(ETD)and Licun River Wastewater Treatment Plant(ELR)were investigated.The results showed that both C.aponinum OUC1 and S.obliquus have superior growth performances in both undiluted effluents,while the better of them was that in ETD effluent,with cell densities of C.aponinum OUC1 and S.obliquus increased by 159%and 66%over that of BG11(control),respectively.Regarding nutrient removal,S.obliquus could completely remove inorganic phosphorus,and decrease ammonia nitrogen in ETD effluent by 81%.In addition,both C.aponinum OUC1 and S.obliquus cultivated in ETD exhibited extraordinary potential for biofuel production,increasing lipid productivities by 133%and 89%of that cultivated in ELR,respectively.As to ultrastructural changes,the differences in the lipoidal globules and glycogen granules of S.obliquus and C.aponinum OUC1 among the ETD and ELR treatments were mostly related to phosphorus limitations.The findings from this research reveal the probability using the secondary effluents as cultivation media to enhance algal biomass,nutrient removal and lipid productivity.
基金supported by the National Key Technology R&D Programme (No. 2011BAD14B04)。
文摘This study determined growth and lipid accumulation in Nannochloropsis sp. MASCC 11 cultivated at different pH, temperatures, and CO2 concentrations in 10-day period. The suitability for biodiesel production was also evaluated based on the fatty acid profiles of microalgae lipid. Nannochloropsis sp. MASCC 11 showed an excellent tolerance to acidic pH(as low as 4.0), high temperatures(at least 40℃), and high CO2 concentrations(5%-15%), which are major stressed conditions in flue gas. The highest algal biomass was acquired at pH of 9.0(0.44 g L^-1), a temperature of 35℃(0.63 g L^-1), and a CO2 concentration of 5%(2.27 g L^-1). Maximum lipid production was obtained at p H of 6.0(108.2 mg L^-1), a temperature of 35℃(134.6 mg L^-1), and a CO2 concentration of 5%(782.7 mg L^-1). Synthesis of polyunsaturated fatty acids(PUFAs) in biomass was stimulated under high CO_2 concentrations, remaining above 80% of total fatty acids, mainly composed of C16:3, C18:2, and C18:3. This led to the algae-based biodiesel having a lower oxidation stability, better cold flow properties, and other parameters such as its kinematic viscosity, cetane number, and specific gravity complied with ASTM or EN 14214 biodiesel specifications. Therefore, the improvement of oxidative stability needs to be considered before Nannochloropsis sp. MASCC 11 lipid can be used for biodiesel production, even if this species can grow well under stressful conditions.
