Objective: To evaluate the effect of Buyang Huanwu Decoction(补阳还五汤,BYHWD) on glial scar after intracerebral hemorrhage(ICH) and investigate the underlying mechanism.Methods: Collagenase type Ⅶ(0.5 U) was injecte...Objective: To evaluate the effect of Buyang Huanwu Decoction(补阳还五汤,BYHWD) on glial scar after intracerebral hemorrhage(ICH) and investigate the underlying mechanism.Methods: Collagenase type Ⅶ(0.5 U) was injected stereotaxically into right globus pallidus to induce ICH model.One hundred and twenty SpragueDawley rats were randomly divided into 3 groups according to a random number table,including normal group(n=40),ICH model group(n=40) and BYHWD group(n=40),respectively.After ICH,the rats in the BYHWD group were intragastrically administered with BYHWD(4.36 g/kg) once a day for 21 days,while the rats in ICH group were administered with equal volume of distilled water for 21 days,respectively.Double immunolabeling was performed for proliferating cell nuclear antigen(PCNA)+/glial ?brillary acidic protein(GFAP)+ nuclei.The expression of GFAP and leukemia inhibitory factor(LIF) was evaluated by immunohistochemistry and quantitative real-time reverse transcription-polymerase chain reaction(RT-PCR).Results: The astrocytes with hypertrophied morphology around the hematoma was observed on day 3 after ICH.The number of GFAP positive cells and GFAP m RNA levels increased notably on day 3 and reached the peak on day 14 post-ICH(P<0.01).PCNA+/GFAP+ nuclei were observed around the hematoma and reached the peak on day 14 post-ICH(P<0.01).In addition,LIF-positive astrocytes and LIF m RNA level in the hemorrhagic region increased signi?cantly till day 14 post-ICH(P<0.01).However,BYHWD not only reduced the number of PCNA+/GFAP+ nuclei,but also decreased GFAP and LIF levels(P<0.05).Conclusion:BYHWD could attenuate ICH-induced glial scar by downregulating the expression of LIF in the rats.展开更多
Objectives： To evaluate the effectiveness and safety of Guipi Decoction（归脾汤, GPD） as an adjunctive in the treatment of depression. Methods： A review of all relevant studies retrieved from a search of the follow...Objectives： To evaluate the effectiveness and safety of Guipi Decoction（归脾汤, GPD） as an adjunctive in the treatment of depression. Methods： A review of all relevant studies retrieved from a search of the following databases were conducted without any language restriction： Excerpt Medica Database（EMBASE）, Pub Med, Cochrane Central Register of Controlled Trials, China National Knowledge Infrastructure（CNKI）, VIP Information, Wanfang Data, and the Chinese Biomedical Literature Database. Papers published until February 2013 were taken into consideration. The analysis was performed using the Cochrane software Revman 5.1. Results： Nine randomized controlled trials involving 620 patients with depression were included in this review. The meta-analysis revealed that compared with antidepressant therapy alone, treatment with a combination of GPD and an antidepressant drug significantly improved the symptoms of depression [weighted mean difference（WMD）： –3.09; 95% confidence interval（CI）： –4.11 to –2.07] and increased the rates of effectiveness（OR： 4.75; 95% CI： 2.66–8.51） as well as recovery（OR： 1.73; 95% CI： 1.17–2.56）. The adverse effects of GPD were not found to be significant in these studies. Conclusions： The findings of this meta-analysis were in keeping with the notion that GPD formulations were effective in the treatment of depression without causing any severe adverse effects. However, currently available evidence was of low quality and therefore inadequate to justify a strong recommendation of using GPD formulations in the management of depression.展开更多
基金the National Natural Science Foundation of China(No.81202625,30400581,30873221 and 81173175)the Project for New Century Excellent Talents(NCET-11-0522)+2 种基金the Hunan Provincial Natural Science Foundation(No.07JJ5007 and 10JJ2023)the Hubei Provincial Natural Science Foundation(No.2017CFB468)the Key Laboratory of Cardiovascular and Cerebrovascular Diseases Translational Medicine(Three Gorges University,No.2016KXN06)
文摘Objective: To evaluate the effect of Buyang Huanwu Decoction(补阳还五汤,BYHWD) on glial scar after intracerebral hemorrhage(ICH) and investigate the underlying mechanism.Methods: Collagenase type Ⅶ(0.5 U) was injected stereotaxically into right globus pallidus to induce ICH model.One hundred and twenty SpragueDawley rats were randomly divided into 3 groups according to a random number table,including normal group(n=40),ICH model group(n=40) and BYHWD group(n=40),respectively.After ICH,the rats in the BYHWD group were intragastrically administered with BYHWD(4.36 g/kg) once a day for 21 days,while the rats in ICH group were administered with equal volume of distilled water for 21 days,respectively.Double immunolabeling was performed for proliferating cell nuclear antigen(PCNA)+/glial ?brillary acidic protein(GFAP)+ nuclei.The expression of GFAP and leukemia inhibitory factor(LIF) was evaluated by immunohistochemistry and quantitative real-time reverse transcription-polymerase chain reaction(RT-PCR).Results: The astrocytes with hypertrophied morphology around the hematoma was observed on day 3 after ICH.The number of GFAP positive cells and GFAP m RNA levels increased notably on day 3 and reached the peak on day 14 post-ICH(P<0.01).PCNA+/GFAP+ nuclei were observed around the hematoma and reached the peak on day 14 post-ICH(P<0.01).In addition,LIF-positive astrocytes and LIF m RNA level in the hemorrhagic region increased signi?cantly till day 14 post-ICH(P<0.01).However,BYHWD not only reduced the number of PCNA+/GFAP+ nuclei,but also decreased GFAP and LIF levels(P<0.05).Conclusion:BYHWD could attenuate ICH-induced glial scar by downregulating the expression of LIF in the rats.
基金Supported by the Natural Science Foundation of Hunan Proince(No.14JJ2024)Traditional Chinese Medicine Science Research Foundation of Health Department of Hunan Province,China(No.201346)
文摘Objectives： To evaluate the effectiveness and safety of Guipi Decoction（归脾汤, GPD） as an adjunctive in the treatment of depression. Methods： A review of all relevant studies retrieved from a search of the following databases were conducted without any language restriction： Excerpt Medica Database（EMBASE）, Pub Med, Cochrane Central Register of Controlled Trials, China National Knowledge Infrastructure（CNKI）, VIP Information, Wanfang Data, and the Chinese Biomedical Literature Database. Papers published until February 2013 were taken into consideration. The analysis was performed using the Cochrane software Revman 5.1. Results： Nine randomized controlled trials involving 620 patients with depression were included in this review. The meta-analysis revealed that compared with antidepressant therapy alone, treatment with a combination of GPD and an antidepressant drug significantly improved the symptoms of depression [weighted mean difference（WMD）： –3.09; 95% confidence interval（CI）： –4.11 to –2.07] and increased the rates of effectiveness（OR： 4.75; 95% CI： 2.66–8.51） as well as recovery（OR： 1.73; 95% CI： 1.17–2.56）. The adverse effects of GPD were not found to be significant in these studies. Conclusions： The findings of this meta-analysis were in keeping with the notion that GPD formulations were effective in the treatment of depression without causing any severe adverse effects. However, currently available evidence was of low quality and therefore inadequate to justify a strong recommendation of using GPD formulations in the management of depression.
