We introduce a first-principles density-functional theory,i.e.the finite-difference pseudopotential density- functional theory in real space and the Langevin molecular dynamics annealing technique,to the descriptions ...We introduce a first-principles density-functional theory,i.e.the finite-difference pseudopotential density- functional theory in real space and the Langevin molecular dynamics annealing technique,to the descriptions of structures and some properties of small carbon clusters(C_N,N=2~8).It is shown that the odd-numbered clusters have linear structures and most of the even-numbered clusters prefer cyclic structures.展开更多
Our previous study showed that two MKN45 variants, namely MKN45Lm-and MKN 45Lm+ selected by laminin adhesion in vitro, had different abilities of invasion and metastasis in vivo (inoculated in nude mice) and in vitro ...Our previous study showed that two MKN45 variants, namely MKN45Lm-and MKN 45Lm+ selected by laminin adhesion in vitro, had different abilities of invasion and metastasis in vivo (inoculated in nude mice) and in vitro (in Boyden chamber), also had different expression of cysteine proteinase. In the present study, we investigated the cell apoptosis of this MKN45 variants by TUNEL and FACS methods. The results were shown significant different of cell apoptosis in this cell sublines. The MKN45Lm- cells had a small subdiploidy peak (3.5%) in DNA content analyzed by flow cytometry,展开更多
基金The project supported by National Natural Science Foundation of China under Grant No.10274055the Research Fund for the Doctoral Program of High Education of China under Grant No.20020610001
文摘We introduce a first-principles density-functional theory,i.e.the finite-difference pseudopotential density- functional theory in real space and the Langevin molecular dynamics annealing technique,to the descriptions of structures and some properties of small carbon clusters(C_N,N=2~8).It is shown that the odd-numbered clusters have linear structures and most of the even-numbered clusters prefer cyclic structures.
文摘Our previous study showed that two MKN45 variants, namely MKN45Lm-and MKN 45Lm+ selected by laminin adhesion in vitro, had different abilities of invasion and metastasis in vivo (inoculated in nude mice) and in vitro (in Boyden chamber), also had different expression of cysteine proteinase. In the present study, we investigated the cell apoptosis of this MKN45 variants by TUNEL and FACS methods. The results were shown significant different of cell apoptosis in this cell sublines. The MKN45Lm- cells had a small subdiploidy peak (3.5%) in DNA content analyzed by flow cytometry,