Ethylene receptors play important roles not only in regulation of growth and development but also in response to environmental stimuli of plants. However, there are few reports on ethylene receptors in soybean. In thi...Ethylene receptors play important roles not only in regulation of growth and development but also in response to environmental stimuli of plants. However, there are few reports on ethylene receptors in soybean. In this article, putative ethylene receptors of soybean were searched from soybean genomic database （http：//www.phytozome.net/search.php） and analyzed. The ethylene receptor gene family in soybean comprising eight members, designated as GmERSI-1, GmERS1-2, GmETRI-1, GmETR1-2, GmETR2-1, GmETR2-2, GmEIN4-1, and GmEIN4-2 corresponding with their homologous genes in drabidopsis, were isolated and analyzed. Phylogenetic analysis indicated that the eight soybean ethylene receptors （SERs） were in two subfamilies and further divided into four groups, viz., groups I （GmERSI-1 and GmERS1-2）, 1I （GmETRI-1 and GmETR1-2）, VI （GmETR2-I and GmETR2-2）, and VII （GmEIN4-1 and GmEIN4-2）. Protein structure of the members in groups I and II from subfamily I were more conserved than the members in other two groups from subfamily II. Expression patterns of the SERs were compared with the homologous genes in Arabidopsis. The results demonstrated that expression patterns of the SERs differed from Arabidopsis members in the same group, suggesting that SERs are involved in different signal pathways compared to ethylene receptors in drabidopsis. Promoter analysis showed that the sequences of the members in each group were different from each other, and some specific binding elements of transcription factors detected in promoter sequences might explain the differences between the members in the same group. A novel soybean TPR protein （tetratricopeptide repeat protein）, GmTPR, was identified to interact with GmETRI-1, apparently an important ethylene receptor in ethylene signaling pathway in soybean. This suggested that GmTPR might be a novel downstream component of the ethylene signaling pathway.展开更多
The expression of β-D-glucuronidase(Gus)gene under the control of soybean heat shock protein (HSP)promoter Gmhsp17. 5-C was investigated in transgenic Nicotiana plumbaginifolia plants after heat shock treatment. Resu...The expression of β-D-glucuronidase(Gus)gene under the control of soybean heat shock protein (HSP)promoter Gmhsp17. 5-C was investigated in transgenic Nicotiana plumbaginifolia plants after heat shock treatment. Results of histochemical stain assay showed that the Gus activity increased markedly during the recovery phase(21℃)after heat shock treatment at 42℃ for 2 h. Results of quantitative fluorometric assays indicated that maximal Gus activity after heat shock treatment could reach 5-6 times higher than that before heat shock treatment in transgenic lines HG6 and HG9. Based on several advantages of the heat shock promoter such as easy to induce, sensitivity to heat shock and high inductive activity, it can be potentially used to control expression of gene of interest in transgenic plants.展开更多
The economic progress of the innovation and development of science and technology, surveying and mapping technology has obtained the diversified development and application. The surveying and mapping technology is gra...The economic progress of the innovation and development of science and technology, surveying and mapping technology has obtained the diversified development and application. The surveying and mapping technology is gradually in the direction of automation, intelligent and efficient. The application of new surveying and mapping technology not only make surveying and mapping simpler, but also improve the efficiency and accuracy of the staff. However, different new surveying and mapping technologies have their own advantages and disadvantages, so the use of new surveying and mapping technologies in surveying and mapping engineering needs to combine the measurement environment and the specific situation of the measuring objects to select, so as to ensure the accuracy and efficiency of the surveying and mapping effect.展开更多
基金funded in part by the National Key Project for Research on Transgenic Biology (2011ZX08002-002 and 2011ZX08002-005)the National High-Tech R&D Program of China (2012AA10A309)
文摘Ethylene receptors play important roles not only in regulation of growth and development but also in response to environmental stimuli of plants. However, there are few reports on ethylene receptors in soybean. In this article, putative ethylene receptors of soybean were searched from soybean genomic database （http：//www.phytozome.net/search.php） and analyzed. The ethylene receptor gene family in soybean comprising eight members, designated as GmERSI-1, GmERS1-2, GmETRI-1, GmETR1-2, GmETR2-1, GmETR2-2, GmEIN4-1, and GmEIN4-2 corresponding with their homologous genes in drabidopsis, were isolated and analyzed. Phylogenetic analysis indicated that the eight soybean ethylene receptors （SERs） were in two subfamilies and further divided into four groups, viz., groups I （GmERSI-1 and GmERS1-2）, 1I （GmETRI-1 and GmETR1-2）, VI （GmETR2-I and GmETR2-2）, and VII （GmEIN4-1 and GmEIN4-2）. Protein structure of the members in groups I and II from subfamily I were more conserved than the members in other two groups from subfamily II. Expression patterns of the SERs were compared with the homologous genes in Arabidopsis. The results demonstrated that expression patterns of the SERs differed from Arabidopsis members in the same group, suggesting that SERs are involved in different signal pathways compared to ethylene receptors in drabidopsis. Promoter analysis showed that the sequences of the members in each group were different from each other, and some specific binding elements of transcription factors detected in promoter sequences might explain the differences between the members in the same group. A novel soybean TPR protein （tetratricopeptide repeat protein）, GmTPR, was identified to interact with GmETRI-1, apparently an important ethylene receptor in ethylene signaling pathway in soybean. This suggested that GmTPR might be a novel downstream component of the ethylene signaling pathway.
基金supported by the National High Tech R&D Program of China(863 Program,2002AA2244011)the National Special Program for Research and Industrialization of Ttansgenic Plants,China(J2000-B-021).
文摘The expression of β-D-glucuronidase(Gus)gene under the control of soybean heat shock protein (HSP)promoter Gmhsp17. 5-C was investigated in transgenic Nicotiana plumbaginifolia plants after heat shock treatment. Results of histochemical stain assay showed that the Gus activity increased markedly during the recovery phase(21℃)after heat shock treatment at 42℃ for 2 h. Results of quantitative fluorometric assays indicated that maximal Gus activity after heat shock treatment could reach 5-6 times higher than that before heat shock treatment in transgenic lines HG6 and HG9. Based on several advantages of the heat shock promoter such as easy to induce, sensitivity to heat shock and high inductive activity, it can be potentially used to control expression of gene of interest in transgenic plants.
文摘The economic progress of the innovation and development of science and technology, surveying and mapping technology has obtained the diversified development and application. The surveying and mapping technology is gradually in the direction of automation, intelligent and efficient. The application of new surveying and mapping technology not only make surveying and mapping simpler, but also improve the efficiency and accuracy of the staff. However, different new surveying and mapping technologies have their own advantages and disadvantages, so the use of new surveying and mapping technologies in surveying and mapping engineering needs to combine the measurement environment and the specific situation of the measuring objects to select, so as to ensure the accuracy and efficiency of the surveying and mapping effect.
基金This work was supported by the National Basic Research Program of China(Grant No.2001CB108904)the Hi-Tech Research and Development Program of China(Grant No.2001AA214021).
