目的:用基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption/ionization-time of flight mass spectrometry,MALDI-TOF MS)检测碳青霉烯耐药的肺炎克雷伯菌(carbapenem-resistant Klebsiella pneumoniae,CRKp)同源性,...目的:用基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption/ionization-time of flight mass spectrometry,MALDI-TOF MS)检测碳青霉烯耐药的肺炎克雷伯菌(carbapenem-resistant Klebsiella pneumoniae,CRKp)同源性,并与脉冲场凝胶电泳(pulsed-field gel electrophoresis,PFGE)和多位点序列分型(multilocus sequence typing,MLST)两种检测方法进行对比,探讨质谱在日常工作中的应用价值。方法:选取2017年10—12月从河南科技大学第一附属医院院患者分离的以及ICU环境采集的碳青霉烯耐药肺炎克雷伯菌10株,检测8种耐药基因:blaTEM、blaSHV、blaCTX-M、blaKPC、blaIMP、blaNDM、blaVIM、blaOXA。采用PFGE、MLST和MALDI-TOF MS 3种方法对这些菌株进行同源性分析。结果:PFGE证实10株CRKp均为同一克隆株的传播,ST型同属于ST11。MALDI-TOF MS经过对细菌进行聚类分析和主成分分析可以区分不同型别肺炎克雷伯菌,对同型肺炎克雷伯菌(Klebsiella pneumonia,Kp)的同源性检测在80%左右。结论:MALDI-TOF MS在肺炎克雷伯菌的同源性检测方面可以应用于院内爆发局部感染的监测,与PFGE和MLST两种方法检测结果一致,但需要尽快建立规范的判断标准。展开更多
Flexibility is the demand of management revolution,and also the milestone of management development.The flexible ERP system is the information system to orientate variety and handle uncertainties.The market with rapid...Flexibility is the demand of management revolution,and also the milestone of management development.The flexible ERP system is the information system to orientate variety and handle uncertainties.The market with rapid change and agile manufacture need a flexible ERP system.The article elaborates the meanings and characteristics about flexibility,the flexibility of ERP and the flexible ERP system;it discussed the necessity,the possibility and the related condition of setting up the flexible ERP system;and puts forward the strategy and the approaches of setting up the flexible ERP system.展开更多
文摘目的:用基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption/ionization-time of flight mass spectrometry,MALDI-TOF MS)检测碳青霉烯耐药的肺炎克雷伯菌(carbapenem-resistant Klebsiella pneumoniae,CRKp)同源性,并与脉冲场凝胶电泳(pulsed-field gel electrophoresis,PFGE)和多位点序列分型(multilocus sequence typing,MLST)两种检测方法进行对比,探讨质谱在日常工作中的应用价值。方法:选取2017年10—12月从河南科技大学第一附属医院院患者分离的以及ICU环境采集的碳青霉烯耐药肺炎克雷伯菌10株,检测8种耐药基因:blaTEM、blaSHV、blaCTX-M、blaKPC、blaIMP、blaNDM、blaVIM、blaOXA。采用PFGE、MLST和MALDI-TOF MS 3种方法对这些菌株进行同源性分析。结果:PFGE证实10株CRKp均为同一克隆株的传播,ST型同属于ST11。MALDI-TOF MS经过对细菌进行聚类分析和主成分分析可以区分不同型别肺炎克雷伯菌,对同型肺炎克雷伯菌(Klebsiella pneumonia,Kp)的同源性检测在80%左右。结论:MALDI-TOF MS在肺炎克雷伯菌的同源性检测方面可以应用于院内爆发局部感染的监测,与PFGE和MLST两种方法检测结果一致,但需要尽快建立规范的判断标准。
文摘Flexibility is the demand of management revolution,and also the milestone of management development.The flexible ERP system is the information system to orientate variety and handle uncertainties.The market with rapid change and agile manufacture need a flexible ERP system.The article elaborates the meanings and characteristics about flexibility,the flexibility of ERP and the flexible ERP system;it discussed the necessity,the possibility and the related condition of setting up the flexible ERP system;and puts forward the strategy and the approaches of setting up the flexible ERP system.
