期刊文献+
共找到4篇文章
< 1 >
每页显示 20 50 100
外泌体miRNA在非小细胞肺癌中的研究进展
1
作者 董鑫鑫 陈阿惠 +1 位作者 李涛 翟乃亮 《滨州医学院学报》 2026年第1期98-103,共6页
肺癌是全球发病率和死亡率最高的恶性肿瘤之一,其中非小细胞肺癌(non-small cell lung cancer,NSCLC)约占所有肺癌比例的85%以上。由于肺癌早期症状隐匿,约70%的患者确诊时已进展至中晚期,导致肺癌患者整体5年生存率不足20%,因此早期诊... 肺癌是全球发病率和死亡率最高的恶性肿瘤之一,其中非小细胞肺癌(non-small cell lung cancer,NSCLC)约占所有肺癌比例的85%以上。由于肺癌早期症状隐匿,约70%的患者确诊时已进展至中晚期,导致肺癌患者整体5年生存率不足20%,因此早期诊断成为改善预后的关键环节。外泌体是大多数细胞都能分泌的一类细胞外囊泡,直径范围约为40~160 nm。其广泛分布在各种生物体液中,包括血液、唾液、尿液、脑脊液、乳汁等。外泌体在细胞和器官之间运输脂质、蛋白质和核酸,是可在细胞之间进行物质交换及信号传递的一种纳米级的天然载体。其中微小RNA(miRNA)可参与调节肿瘤细胞的发生发展、侵袭和转移等过程,具有成为肿瘤生物标志物的潜力,近年来在肺癌的发生、诊断、治疗和预后中的作用引起了人们的广泛关注。本文综述了外泌体中miRNA在NSCLC的诊断和鉴别、治疗及预后中的作用。 展开更多
关键词 外泌体 微小RNA 非小细胞肺癌 生物标志物
暂未订购
细胞爬片式共培养模型的建立和优点
2
作者 陈阿惠 李涛 +1 位作者 董鑫鑫 翟乃亮 《滨州医学院学报》 2025年第2期125-129,共5页
目的探究不同共培养模式下人肺腺癌A549细胞对人脐静脉内皮融合细胞EA.hy.926细胞表型及功能的影响以寻找更适合模拟肿瘤微环境的共培养模型。方法分别利用细胞爬片和Transwell小室共培养法建立A549细胞与EA.hy.926细胞的共培养模型,通... 目的探究不同共培养模式下人肺腺癌A549细胞对人脐静脉内皮融合细胞EA.hy.926细胞表型及功能的影响以寻找更适合模拟肿瘤微环境的共培养模型。方法分别利用细胞爬片和Transwell小室共培养法建立A549细胞与EA.hy.926细胞的共培养模型,通过FITC-葡聚糖细胞渗透实验评估内皮细胞屏障完整性,Western blot检测内皮细胞多糖包被核心蛋白SDC-1、紧密连接蛋白ZO-1、闭合蛋白(Occludin)相关蛋白表达及CCK8实验检测内皮细胞增殖活性,以分析两种共培养模型下EA.hy.926细胞的表型及功能变化。结果与Transwell小室共培养模型相比:细胞爬片式共培养模型中,EA.hy.926细胞渗透率显著增加(P<0.05),提示内皮细胞完整性破坏,细胞通透性增加;通过Western blot分析,在细胞爬片式共培养模型中EA.hy.926细胞的细胞屏障相关蛋白SDC-1、ZO-1、Occludin蛋白表达下调,破坏明显(P<0.05);细胞爬片式共培养模型中EA.hy.926细胞的增殖活性显著增加(P<0.05)。结论与Transwell小室共培养模型相比,细胞爬片共培养模型能够更真实地模拟肿瘤细胞对内皮细胞的作用,为肺癌转移研究提供了更优的体外研究模型。 展开更多
关键词 肺癌 内皮细胞 多配体蛋白聚糖-1 细胞连接 细胞骨架 细胞共培养
暂未订购
意义赋值、资源重塑和技术赋能:档案虚拟仿真实验教学改革研究与实践 被引量:11
3
作者 高大伟 陈阿辉 +1 位作者 丁晓雪 张伟茵 《档案学研究》 CSSCI 北大核心 2021年第5期60-65,共6页
档案虚拟仿真实验教学改革是信息技术与实验教学深度融合的体现,是响应国家虚拟仿真"金课"建设的必要行动和解决实验教学问题的重要方向。教学改革应遵循价值、技术和资源体系要求,实现意义赋值、资源重塑和技术赋能。本文以&... 档案虚拟仿真实验教学改革是信息技术与实验教学深度融合的体现,是响应国家虚拟仿真"金课"建设的必要行动和解决实验教学问题的重要方向。教学改革应遵循价值、技术和资源体系要求,实现意义赋值、资源重塑和技术赋能。本文以"档案展览集成开发"项目建设为例,提出了项目确定方法、内容设计思路和系统建设要点。 展开更多
关键词 实验教学 虚拟仿真 档案展览 金课
原文传递
Impact of land use change on carbon storage based on the PLUS–InVEST model:A case study in the urban belt along the Yellow River,China
4
作者 SHI Hanqi DUAN Huan'e +3 位作者 LI Xuemei WANG Guigang chen ahui LIANG Dengrui 《Journal of Arid Land》 2026年第3期452-476,共25页
Terrestrial ecosystems are vital for maintaining equilibrium in the global carbon cycle.Land use and land cover change(LUCC),which is influenced mainly by urbanization and ecological policies,impacts terrestrial ecosy... Terrestrial ecosystems are vital for maintaining equilibrium in the global carbon cycle.Land use and land cover change(LUCC),which is influenced mainly by urbanization and ecological policies,impacts terrestrial ecosystem carbon storage significantly.In this study,spatiotemporal carbon storage changes in the urban belt along the Yellow River in the Ningxia Hui Autonomous Region,China,were estimated through a model that integrated patch-generating land use simulation(PLUS)and integrated valuation of ecosystem services and tradeoffs(InVEST)models from 1993 to 2033.The results revealed that:(1)from 1993 to 2023,the expansion of built-up land and cropland was derived mainly from unused land and grassland,whereas water body and woodland remained relatively stable.Projections to 2033 have indicated that LUCC will continue and be concentrated primarily in the Ningxia Plain;(2)carbon storage increased by a net 5.01×10^(6) Mg C from 1993 to 2023;(3)the spatial distribution of carbon storage revealed that high-value areas were predominantly located in the Helan Mountains and the Ningxia Plain,whereas low-value areas were found in the Tengger Desert;(4)scenario projections indicated that by 2033,the ecological protection scenario(EPS)would achieve a 0.18×10^(6) Mg C increase by reducing the conversion of woodland to cropland and grassland to built-up land,while increasing the conversion of unused land to grassland.In contrast,the natural development scenario(NDS),cropland protection scenario(CPS),and urban development scenario(UDS)decreased carbon storage by 0.60×10^(6),0.21×10^(6),and 0.42×10^(6) Mg C,respectively;and(5)spatial autocorrelation analysis revealed that high–high carbon storage clusters formed belt-like patterns along the Ningxia Plain and the Helan Mountains,whereas the low–low carbon storage clusters were concentrated in northern Zhongwei City,western Qingtongxia City,western Dawukou District,and the urbanized areas within the central Ningxia Plain.Overall,the study results revealed the close coupling relationship between LUCC and carbon storage functions.Furthermore,the study establishes a framework for carbon management that balances ecological protection with coordinated urban development for the urban belt as well as for similar arid and semi-arid areas.On the basis of these findings,this study provides decision-makers with guidance to optimize ecosystem carbon storage via land use,which plays a key role in developing future land use policies and achieving the"dual carbon"goals. 展开更多
关键词 carbon storage land use change patch-generating land use simulation(PLUS)model integrated valuation of ecosystem services and tradeoffs(InVEST)model Moran's I ecological protection
在线阅读 下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部