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浙江省一规模猪场副猪嗜血杆菌的分离鉴定
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作者 周蕾 虞一聪 +8 位作者 董建斐 柴娟 王雅婷 刘爱军 谢荣辉 倪柏锋 张传亮 赵灵燕 桂平雄 《中国动物检疫》 CAS 2018年第7期82-85,共4页
2017年12月,浙江省杭州市萧山区某规模猪场保育舍猪群出现高热(41.5℃)、咳嗽、腹式呼吸、关节肿胀伴有跛行和被毛粗乱等临床症状。为确诊疾病,明确病因,剖检2头40日龄病死猪,用接种环刮取肺脏病料接种血平板,再用金黄色葡萄球菌作交叉... 2017年12月,浙江省杭州市萧山区某规模猪场保育舍猪群出现高热(41.5℃)、咳嗽、腹式呼吸、关节肿胀伴有跛行和被毛粗乱等临床症状。为确诊疾病,明确病因,剖检2头40日龄病死猪,用接种环刮取肺脏病料接种血平板,再用金黄色葡萄球菌作交叉划线,于37℃5%CO2条件下培养24 h。结果显示:病料培养血平板上生长出大量半透明、不溶血小菌落,菌落直径为0.5~1 mm;靠近金黄色葡萄球菌菌苔的菌落较大,随着与葡萄球菌生长线距离的增加而变小或不生长,呈"卫星现象"。挑取可疑菌落进行纯化培养,将纯培养物制成菌悬液,以DNA纯化试剂盒提取DNA并进行PCR检测。扩增产物电泳后,被检菌株电泳道出现一条1 090 bp条带,表明该菌株为副猪嗜血杆菌。 展开更多
关键词 副猪嗜血杆菌 细菌鉴定 PCR 规模猪场
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Study on the Effect of Oral Care on Improving Oral Diseases
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作者 chaijuan 《外文科技期刊数据库(文摘版)医药卫生》 2022年第4期154-157,共4页
Objective: to explore the effective treatment of oral diseases through the change of nursing methods. Methods: 60 patients who were hospitalized in the Department of Stomatology of our hospital from January to August ... Objective: to explore the effective treatment of oral diseases through the change of nursing methods. Methods: 60 patients who were hospitalized in the Department of Stomatology of our hospital from January to August 2019 were randomly divided into three groups: A, B and C, with 20 patients in each group. Group A, B and C patients with oral diseases were given cotton ball wiping care, result classification and cotton ball combined care result classification. The rehabilitation effects of three groups of patients under different nursing measures were compared. Results: after two weeks of nursing, the effective rate of Group C was Group A and Group B, the data was statistically significant (P<0.05). After four weeks of nursing, the effective rates were Group C and Group B, Group A, the data were statistically significant (P<0.05). Conclusion: for patients with oral diseases, the cotton pad wiping method can achieve good nursing effect in the first two weeks. In the fourth week, the nursing effect of the classification of nursing results was significantly higher than that of oral wiping method. The nursing effect of wiping the oral cavity combined with the classification of nursing results is higher. Therefore, the nursing of oral diseases should adopt the method of cotton swab combined with the classification of nursing results. 展开更多
关键词 cotton ball wiping method classification of nursing outcomes patients with oral diseases
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