OBJECTIVE: To evaluate the effects of Zuyangping(足疡平, ZYP) formula on wound healing in diabetic rats, as well as the molecular mechanisms involved. METHODS: The main compounds in ZYP formula were identified by the ...OBJECTIVE: To evaluate the effects of Zuyangping(足疡平, ZYP) formula on wound healing in diabetic rats, as well as the molecular mechanisms involved. METHODS: The main compounds in ZYP formula were identified by the Liquid chromatography-tandem mass spectrometry. Sprague-Dawley rats, injected with streptozotocin(STZ) to establish diabetes model, then, formed a defective skin trauma in the back, and each group was treated with corresponding drugs once a day. Granulation was taken from each time node for histological analysis. The Western blotting was used to measured protein expression of advanced glycation end products receptor(RAGE) and hypoxia-inducible factor-1α(HIF-1α) axis-related proteins. The relative expression levels of inflammatory cytokines and growth factors were measured by the enzyme-linked immunosorbent assay method. RESULTS: The main ingredients were identified in the ZYP formula. Histological analysis showed that the ZYP formula could inhibit the expression of inflammation, promote angiogenesis and collagen deposition. In addition, the ZYP formula could regulate the expression of RAGE and HIF1-α axis-related proteins, thus promoting the wound healing in diabetic rats. CONCLUSION: The ZYP formula could accelerate wound healing in diabetic rats.展开更多
基金the Hubei Provincial Central Guidance Local Science and Technology Development Project:Wuhan Precision Diagnosis and Treatment Clinical Medical Research Center for Severe Infections (No. 2020ZYYD026)Wuhan Municipal Health Commission Scientific Research Project:Study on the effect of Zuyangping formula on Diabetes Wound Healing through Advanced Glycation End Products Receptor/Nuclear Factor Kappa-B p65/Nucleotide-binding Oligomerization Domain,Leucine-Rich Repeat and Pyrin Domain-Containing 3 Mediated Inflammatory Reaction (No. WZ22A01)+1 种基金Wuhan Applied Basic Frontier Project:Development of Scar Eliminating Cream for Burn Scar Clinical Agreement (No. 2020020601012301)Wuhan Clinical Medical Research Project:Preclinical Study of Mitogenactivated Protein Kinase 12-based Pituitary Prolactinoma and Adrenocorticotropin Adenoma in the Treatment of New Drug Barley Maltamine (No. WX20M02)。
文摘OBJECTIVE: To evaluate the effects of Zuyangping(足疡平, ZYP) formula on wound healing in diabetic rats, as well as the molecular mechanisms involved. METHODS: The main compounds in ZYP formula were identified by the Liquid chromatography-tandem mass spectrometry. Sprague-Dawley rats, injected with streptozotocin(STZ) to establish diabetes model, then, formed a defective skin trauma in the back, and each group was treated with corresponding drugs once a day. Granulation was taken from each time node for histological analysis. The Western blotting was used to measured protein expression of advanced glycation end products receptor(RAGE) and hypoxia-inducible factor-1α(HIF-1α) axis-related proteins. The relative expression levels of inflammatory cytokines and growth factors were measured by the enzyme-linked immunosorbent assay method. RESULTS: The main ingredients were identified in the ZYP formula. Histological analysis showed that the ZYP formula could inhibit the expression of inflammation, promote angiogenesis and collagen deposition. In addition, the ZYP formula could regulate the expression of RAGE and HIF1-α axis-related proteins, thus promoting the wound healing in diabetic rats. CONCLUSION: The ZYP formula could accelerate wound healing in diabetic rats.
