Iron is an essential nutrient for plant growth,development,and disease resistance.Plants absorb iron through their roots,with citrate playing a key role in xylem transport of insoluble Fe3+.In this study,we identified...Iron is an essential nutrient for plant growth,development,and disease resistance.Plants absorb iron through their roots,with citrate playing a key role in xylem transport of insoluble Fe3+.In this study,we identified the cytoplasmic ATP-citrate lyase(ACL)subunit A2 in rice(Oryza sativa L.),OsACL-A2(Os12g0566300),as a critical factor for iron uptake and transport.The osacl-a2 mutant exhibited reduced leaf iron levels,leading to iron deficiency-induced chlorosis,activated defense signaling,and eventual necrosis in mature leaves.Additionally,blast resistance was weakened in immature osacl-a2 leaves.Exogenous iron supplementation rescued these defects.The mutant displayed reduced ATP-citrate lyase activity but increased citric acid levels compared with its wild type(WT),suggesting that the osacl-a2 mutation impairs enzyme activity.Thus,OsACL-A2-mediated citrate lyase activity plays a vital role in promoting iron uptake and associated blast resistance in rice.展开更多
Grain size is one of key agronomic traits associated with grain yield and grain quality. Both major quantitative trait loci GS3 and GL3.1 play a predominant role in negative regulation of grain size. In this study, a ...Grain size is one of key agronomic traits associated with grain yield and grain quality. Both major quantitative trait loci GS3 and GL3.1 play a predominant role in negative regulation of grain size. In this study, a CRISPR/Cas9-mediated multiplex genome editing system was used to simultaneously edit GS3 and GL3.1 in a typical japonica rice Nipponbare. In T1 generation, we found that gs3 formed slender grain with lower chalkiness percentage, while gs3gl3.1 produced larger grain with higher chalkiness percentage. In terms of other agronomic traits, flag leaf size, grain number and grain yield of both gs3 and gs3gl3.1 mutants were affected. It is noteworthy that gs3 and gs3gl3.1 mutants both led to dramatical reduction of grain number, thereby decreased grain yield. In conclusion, these results indicated that knockout of GS3 and GL3.1 could rapidly improve grain size, but probably have some negative influences on grain quality and grain yield.展开更多
基金supported by the Basic Public Welfare Research Program of Zhejiang Province,China(Grant No.LY23C130003)the Fund of the State Key Laboratory of Rice Biology and Breeding,China(Grant No.2023ZZKT20203)+5 种基金the Major Science and Technology Project of Guangxi,China(Grant No.AA23062015)the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences,China(Grant No.CAAS-ASTIP-2013-CNRRI)the China Rice Research System,China(Grant No.CARS-01-011)the Central Public-Interest Scientific Institution Basal Research Fund,China(Grant No.CPSIBRF-CNRRI-202301)High-Quality and Resistant Hybrid Rice Germplasm Creation and New Varieties Development with International Competitiveness,China(Grant Nos.2022KJCX45 and YBXM2437)Xi’nan League Science and Technology Project,China(Grant No.2023DXZD0001).
文摘Iron is an essential nutrient for plant growth,development,and disease resistance.Plants absorb iron through their roots,with citrate playing a key role in xylem transport of insoluble Fe3+.In this study,we identified the cytoplasmic ATP-citrate lyase(ACL)subunit A2 in rice(Oryza sativa L.),OsACL-A2(Os12g0566300),as a critical factor for iron uptake and transport.The osacl-a2 mutant exhibited reduced leaf iron levels,leading to iron deficiency-induced chlorosis,activated defense signaling,and eventual necrosis in mature leaves.Additionally,blast resistance was weakened in immature osacl-a2 leaves.Exogenous iron supplementation rescued these defects.The mutant displayed reduced ATP-citrate lyase activity but increased citric acid levels compared with its wild type(WT),suggesting that the osacl-a2 mutation impairs enzyme activity.Thus,OsACL-A2-mediated citrate lyase activity plays a vital role in promoting iron uptake and associated blast resistance in rice.
基金supported by the National Nature Science Foundation of China(Grant Nos.31521064 and 31961143016)Technological Innovation Project of Chinese Academy of Agricultural Sciences(Grant No.CAASASTIP-2013-CNRRI)+2 种基金Major Transgenic Projects of China(Grant No.2016ZX08001-002)National Key Research and Development Program(Grant No.2016YFD0101801)the Natural Science Foundation of Innovation Research Group(Grant No.31521064)。
文摘Grain size is one of key agronomic traits associated with grain yield and grain quality. Both major quantitative trait loci GS3 and GL3.1 play a predominant role in negative regulation of grain size. In this study, a CRISPR/Cas9-mediated multiplex genome editing system was used to simultaneously edit GS3 and GL3.1 in a typical japonica rice Nipponbare. In T1 generation, we found that gs3 formed slender grain with lower chalkiness percentage, while gs3gl3.1 produced larger grain with higher chalkiness percentage. In terms of other agronomic traits, flag leaf size, grain number and grain yield of both gs3 and gs3gl3.1 mutants were affected. It is noteworthy that gs3 and gs3gl3.1 mutants both led to dramatical reduction of grain number, thereby decreased grain yield. In conclusion, these results indicated that knockout of GS3 and GL3.1 could rapidly improve grain size, but probably have some negative influences on grain quality and grain yield.
