Carbon quantum dots(CQDs)have a size of 10 nm(or less),with lots of biomedical advantages,creating huge excitement in different research fields.The aim of this study includes an eco-friendly synthesis of biogenic CQDs...Carbon quantum dots(CQDs)have a size of 10 nm(or less),with lots of biomedical advantages,creating huge excitement in different research fields.The aim of this study includes an eco-friendly synthesis of biogenic CQDs from grape(Vitis vinifera)seeds,identifying the characteristics and assessing its anti-diabetic as well as anti-microbial activity.CQDs are prepared by the pyrolysis method.Synthesized CQDs were confirmed by ultraviolet(UV)-visible(Vis)spectrophotometer,and the characterization study was done by X-ray diffractometer,photoluminescence spectroscopy,Fourier transform infra-red spectroscopy,and transmission electron microscopy with selected area electron diffraction(SAED).Anti-diabetic activity of CQDs was analyzed by in vitroα-glycosidase,α-amylase inhibition assays,and glucose uptake studies.The anti-bacterial activity of CQDs was analyzed by anti-microbial assay technique against Escherichia coli,Pseudomonas aeruginosa,Staphylococcus aureus,and Streptococcus mutans.The results showed that the CQDs synthesized from a natural source like grape seeds,were amorphous in nature,the average particle size was 4 nm,and they contain functional groups like carboxyl and hydroxyl.Subsequently,it showed that the sp2 domains also produce green fluorescence.The anti-diabetic experimental method revealed that the CQDs enhance glucose uptake and inhibit carbohydrate hydrolyzing enzymes.CQDs also exhibit anti-bacterial properties against both Gram-positive and Gram-negative bacteria,according to their antimicrobial impact.Due to their small size and higher activity,CQDs will become strong anti-diabetic agents as well as anti-bacterial ones.展开更多
文摘Carbon quantum dots(CQDs)have a size of 10 nm(or less),with lots of biomedical advantages,creating huge excitement in different research fields.The aim of this study includes an eco-friendly synthesis of biogenic CQDs from grape(Vitis vinifera)seeds,identifying the characteristics and assessing its anti-diabetic as well as anti-microbial activity.CQDs are prepared by the pyrolysis method.Synthesized CQDs were confirmed by ultraviolet(UV)-visible(Vis)spectrophotometer,and the characterization study was done by X-ray diffractometer,photoluminescence spectroscopy,Fourier transform infra-red spectroscopy,and transmission electron microscopy with selected area electron diffraction(SAED).Anti-diabetic activity of CQDs was analyzed by in vitroα-glycosidase,α-amylase inhibition assays,and glucose uptake studies.The anti-bacterial activity of CQDs was analyzed by anti-microbial assay technique against Escherichia coli,Pseudomonas aeruginosa,Staphylococcus aureus,and Streptococcus mutans.The results showed that the CQDs synthesized from a natural source like grape seeds,were amorphous in nature,the average particle size was 4 nm,and they contain functional groups like carboxyl and hydroxyl.Subsequently,it showed that the sp2 domains also produce green fluorescence.The anti-diabetic experimental method revealed that the CQDs enhance glucose uptake and inhibit carbohydrate hydrolyzing enzymes.CQDs also exhibit anti-bacterial properties against both Gram-positive and Gram-negative bacteria,according to their antimicrobial impact.Due to their small size and higher activity,CQDs will become strong anti-diabetic agents as well as anti-bacterial ones.
