A 9-month incubation experiment using composted and non-composted amendments derived from vine pruning waste and sewage sludge was carried out to study the effects of the nature and stability of organic amendments on ...A 9-month incubation experiment using composted and non-composted amendments derived from vine pruning waste and sewage sludge was carried out to study the effects of the nature and stability of organic amendments on the structural composition of organic matter (OM) in a semi-arid soil.The changes of soil OM,both in the whole soil and in the extractable carbon with pyrophosphate,were evaluated by pyrolysis-gas chromatography and chemical analyses.By the end of the experiment,the soils amended with pruning waste exhibited less organic carbon loss than those receiving sewage sludge.The non-composted residues increased the aliphatic-pyrolytic products of the OM,both in the whole soil and also in the pyrophosphate extract,with the products derived from peptides and proteins being significantly higher.After 9 months,in the soils amended with pruning waste the relative abundance of phenolic-pyrolytic products derived from phenolic compounds,lignin and proteins in the whole soil tended to increase more than those in the soils amended with sewage sludge.However,the extractable OM with pyrophosphate in the soils amended with composted residues tended to have higher contents of these phenolic-pyrolytic products than that in non-composted ones.Thus,despite the stability of pruning waste,the composting of this material promoted the incorporation of phenolic compounds to the soil OM.The pyrolytic indices (furfural/pyrrole and aliphatic/aromatic ratios) showed the diminution of aliphatic compounds and the increase of aromatic compounds,indicating the stabilization of the OM in the amended soils after 9 months.In conclusion,the changes of soil OM depend on the nature and stability of the organic amendments,with composted vine pruning waste favouring humification.展开更多
By using an instrumented impact pendulum, the force versus time curves of 7075-T651 aluminum welds were obtained from standard Charpy-V samples. Considering the force-time curves and constant impact velocity, the frac...By using an instrumented impact pendulum, the force versus time curves of 7075-T651 aluminum welds were obtained from standard Charpy-V samples. Considering the force-time curves and constant impact velocity, the fracture energies for different zones were quantified. A fracture energy improvement for the HAZ(33.6 J) was observed in comparison with the weld metal(7.88 J), and base metal(5.37 J and 7.37 J for longitudinal and transverse directions, respectively). This toughness increment was attributed to the microstructural transformation caused by the thermodynamic instability of η′ precipitates during the welding. Fracture energy for weld metal was higher than that for base metal, probably due to pores created during solidification. Regarding the dynamic yielding force obtained from the force-time curves, an approximation to the dynamic yield strength for weld, HAZ and base metal was determined. Fracture surfaces revealed an intergranular failure for base metal in longitudinal direction, whereas a predominately brittle failure(cleavage) with some insights of ductile characteristics was observed for the transverse direction. In contrast, a ductile failure was observed for weld metal and HAZ.展开更多
Hydrocarbon contamination may affect the soil microbial community, in terms of both diversity and function. A laboratory experiment was set-up, with a semi-arid control soil and the same soil but artificially contamin...Hydrocarbon contamination may affect the soil microbial community, in terms of both diversity and function. A laboratory experiment was set-up, with a semi-arid control soil and the same soil but artificially contaminated with diesel oil, to follow changes in the dominant species of the microbial community in the hydrocarbon-polluted soil via proteomics. Analysis of the proteins extracted from enriched cultures growing in Luria-Bertani （LB） media showed a change in the microbial community. The majority of the proteins were related to gIycolysis pathways, structural or protein synthesis. The results showed a relative increase in the complexity of the soil microbial community with hydrocarbon contamination, especially after 15 days of incubation. Species such as Ralstonia solanacearum, Synechococcus elongatus and different Clostridium sp. were adapted to contamination, not appearing in the control soil, although Bacillus sp. dominated the growing in LB in any of the treatments. We conclude that the identification of microbial species in soil extracts by culture-dependent proteomics is able to partially explain the changes in the diversity of the soil microbial community in hydrocarbon polluted semi-arid soils, but this information is much more limited than that provided by molecular methods.展开更多
基金Supported by the Spanish Ministry of Science and Innovation (No. CTM 2007-60061)the CSIC-I3P fellowship from the Council of Scientific Research (CSIC) of Spain
文摘A 9-month incubation experiment using composted and non-composted amendments derived from vine pruning waste and sewage sludge was carried out to study the effects of the nature and stability of organic amendments on the structural composition of organic matter (OM) in a semi-arid soil.The changes of soil OM,both in the whole soil and in the extractable carbon with pyrophosphate,were evaluated by pyrolysis-gas chromatography and chemical analyses.By the end of the experiment,the soils amended with pruning waste exhibited less organic carbon loss than those receiving sewage sludge.The non-composted residues increased the aliphatic-pyrolytic products of the OM,both in the whole soil and also in the pyrophosphate extract,with the products derived from peptides and proteins being significantly higher.After 9 months,in the soils amended with pruning waste the relative abundance of phenolic-pyrolytic products derived from phenolic compounds,lignin and proteins in the whole soil tended to increase more than those in the soils amended with sewage sludge.However,the extractable OM with pyrophosphate in the soils amended with composted residues tended to have higher contents of these phenolic-pyrolytic products than that in non-composted ones.Thus,despite the stability of pruning waste,the composting of this material promoted the incorporation of phenolic compounds to the soil OM.The pyrolytic indices (furfural/pyrrole and aliphatic/aromatic ratios) showed the diminution of aliphatic compounds and the increase of aromatic compounds,indicating the stabilization of the OM in the amended soils after 9 months.In conclusion,the changes of soil OM depend on the nature and stability of the organic amendments,with composted vine pruning waste favouring humification.
基金CONACy T (project CB 177834)SIP-IPN for the funds given to conduct this research
文摘By using an instrumented impact pendulum, the force versus time curves of 7075-T651 aluminum welds were obtained from standard Charpy-V samples. Considering the force-time curves and constant impact velocity, the fracture energies for different zones were quantified. A fracture energy improvement for the HAZ(33.6 J) was observed in comparison with the weld metal(7.88 J), and base metal(5.37 J and 7.37 J for longitudinal and transverse directions, respectively). This toughness increment was attributed to the microstructural transformation caused by the thermodynamic instability of η′ precipitates during the welding. Fracture energy for weld metal was higher than that for base metal, probably due to pores created during solidification. Regarding the dynamic yielding force obtained from the force-time curves, an approximation to the dynamic yield strength for weld, HAZ and base metal was determined. Fracture surfaces revealed an intergranular failure for base metal in longitudinal direction, whereas a predominately brittle failure(cleavage) with some insights of ductile characteristics was observed for the transverse direction. In contrast, a ductile failure was observed for weld metal and HAZ.
基金Supported by the JAE-Program for Ph.D. Students of Spanish Research Council
文摘Hydrocarbon contamination may affect the soil microbial community, in terms of both diversity and function. A laboratory experiment was set-up, with a semi-arid control soil and the same soil but artificially contaminated with diesel oil, to follow changes in the dominant species of the microbial community in the hydrocarbon-polluted soil via proteomics. Analysis of the proteins extracted from enriched cultures growing in Luria-Bertani （LB） media showed a change in the microbial community. The majority of the proteins were related to gIycolysis pathways, structural or protein synthesis. The results showed a relative increase in the complexity of the soil microbial community with hydrocarbon contamination, especially after 15 days of incubation. Species such as Ralstonia solanacearum, Synechococcus elongatus and different Clostridium sp. were adapted to contamination, not appearing in the control soil, although Bacillus sp. dominated the growing in LB in any of the treatments. We conclude that the identification of microbial species in soil extracts by culture-dependent proteomics is able to partially explain the changes in the diversity of the soil microbial community in hydrocarbon polluted semi-arid soils, but this information is much more limited than that provided by molecular methods.
