Microalgae and cyanobacteria are photosynthetic microorganisms that inhabit freshwater and marine ecosystems.Bioactive substances(metabolites such as astaxanthin,chlorophyll-a,and phycobiliproteins)obtained from micro...Microalgae and cyanobacteria are photosynthetic microorganisms that inhabit freshwater and marine ecosystems.Bioactive substances(metabolites such as astaxanthin,chlorophyll-a,and phycobiliproteins)obtained from microalgae and cyanobacteria are used in a multitude of fields.Phycobiliproteins are photosynthetic antenna pigments that are found in cyanobacteria,red algae,and cryptophytes.This study aimed to determine the optimal parameters for phycobiliprotein extraction from lyophilized cells obtained from a triple algal co-culture.These parameters included the biomass:solvent ratio,CaCI_(2)concentration,agitation speed,and extraction time.In all optimization processes,phycocyanin is observed to be the most dominant,while phycoerythrin has the lowest amount.It is demonstrated that all phycobiliprotein efficiencies increase after each optimization process.The highest yield of 12.51±0.23 mg phycobiliprotein/g freeze-dried weight was obtained using a 1:100(v:v)biomass:solvent ratio with 2%CaCl_(2)at 100 rpm for 1 h.The significance of carefully controlling extraction parameters to maximize the efficiency of PBP extraction from triple algal co-culture is highlighted by these results.Employing a combination of extraction methods could potentially improve both the yield and purity of phycobiliproteins obtained from a triple algal co-culture.Future research should focus on developing and refining scaling-up techniques to enhance and optimize the extraction process of phycobiliproteins for industrial use.展开更多
Selecting the appropriate algal species is essential to maximize lipid extraction from microalgae.Chlorococcum sp.is a unicellular organism found in both aquatic and terrestrial habitats.Although the influence of nitr...Selecting the appropriate algal species is essential to maximize lipid extraction from microalgae.Chlorococcum sp.is a unicellular organism found in both aquatic and terrestrial habitats.Although the influence of nitrogen on lipid metabolism is well established across various microalgae,its particular effects on Chlorococcum sp.provide novel insights into this relatively underexplored species.Examining how nitrogen affects lipid accumulation in Chlorococcum sp.could reveal new strategies to enhance its application in biodiesel productions.This study aimed to evaluate the lipid content of Chlorococcum novae-angliae and compare the lipid and fatty acid yields between cultures grown in N-supplied and N-starvation culture media.The results show that Chlorococcum sp.cultivated in N-supplied culture medium reached the highest cell count of 2.16±8.18×10^(-8)cells/mL,with a specific growth rate(μ)of 0.55 d^(−1),whereas a cell count of 1.60±6.63×10^(-8)cells/mL was found for the N-starvation culture medium.On the other hand,the highest lipid yield,recorded as 0.098±0.012 g lipid/g wet biomass,mainly consisting of tridecanoic and palmitic acids(77%),was obtained from the N-starvation culture medium.Tridecanoic acid(C13:0)was detected for the first time in C.novae angliae.For the N-supplied culture medium,the lipid yield was 0.082±0.010 g lipid/g wet biomass.Therefore,in cases where maximizing lipid yield is crucial,such as for biofuel production,nitrogen starvation might be a more effective approach,even though it may result in lower overall biomass productivity.However,for applications that prioritize higher biomass,such as animal feed,ensuring sufficient nitrogen levels could be more beneficial.展开更多
基金This study was a part of Cost Action CA20127supported financially by the Scientific and Technological Research Council of Turkey(TUBITAK)under project number 121R100the Scientific and Technological Research Council of Turkey(TUBITAK)for the financial support.
文摘Microalgae and cyanobacteria are photosynthetic microorganisms that inhabit freshwater and marine ecosystems.Bioactive substances(metabolites such as astaxanthin,chlorophyll-a,and phycobiliproteins)obtained from microalgae and cyanobacteria are used in a multitude of fields.Phycobiliproteins are photosynthetic antenna pigments that are found in cyanobacteria,red algae,and cryptophytes.This study aimed to determine the optimal parameters for phycobiliprotein extraction from lyophilized cells obtained from a triple algal co-culture.These parameters included the biomass:solvent ratio,CaCI_(2)concentration,agitation speed,and extraction time.In all optimization processes,phycocyanin is observed to be the most dominant,while phycoerythrin has the lowest amount.It is demonstrated that all phycobiliprotein efficiencies increase after each optimization process.The highest yield of 12.51±0.23 mg phycobiliprotein/g freeze-dried weight was obtained using a 1:100(v:v)biomass:solvent ratio with 2%CaCl_(2)at 100 rpm for 1 h.The significance of carefully controlling extraction parameters to maximize the efficiency of PBP extraction from triple algal co-culture is highlighted by these results.Employing a combination of extraction methods could potentially improve both the yield and purity of phycobiliproteins obtained from a triple algal co-culture.Future research should focus on developing and refining scaling-up techniques to enhance and optimize the extraction process of phycobiliproteins for industrial use.
基金financially supported by Ege University Scientific Research Projects(BAP)with project number 30846Ege University Scientific Research Projects(BAP)for the financial support.
文摘Selecting the appropriate algal species is essential to maximize lipid extraction from microalgae.Chlorococcum sp.is a unicellular organism found in both aquatic and terrestrial habitats.Although the influence of nitrogen on lipid metabolism is well established across various microalgae,its particular effects on Chlorococcum sp.provide novel insights into this relatively underexplored species.Examining how nitrogen affects lipid accumulation in Chlorococcum sp.could reveal new strategies to enhance its application in biodiesel productions.This study aimed to evaluate the lipid content of Chlorococcum novae-angliae and compare the lipid and fatty acid yields between cultures grown in N-supplied and N-starvation culture media.The results show that Chlorococcum sp.cultivated in N-supplied culture medium reached the highest cell count of 2.16±8.18×10^(-8)cells/mL,with a specific growth rate(μ)of 0.55 d^(−1),whereas a cell count of 1.60±6.63×10^(-8)cells/mL was found for the N-starvation culture medium.On the other hand,the highest lipid yield,recorded as 0.098±0.012 g lipid/g wet biomass,mainly consisting of tridecanoic and palmitic acids(77%),was obtained from the N-starvation culture medium.Tridecanoic acid(C13:0)was detected for the first time in C.novae angliae.For the N-supplied culture medium,the lipid yield was 0.082±0.010 g lipid/g wet biomass.Therefore,in cases where maximizing lipid yield is crucial,such as for biofuel production,nitrogen starvation might be a more effective approach,even though it may result in lower overall biomass productivity.However,for applications that prioritize higher biomass,such as animal feed,ensuring sufficient nitrogen levels could be more beneficial.
