Background:Leishmaniasis causes alterations and lesions in the genital system,which leads to azoospermia and testicular atrophy in animals during the chronic phase of the infection.The aim of this study was to reveal ...Background:Leishmaniasis causes alterations and lesions in the genital system,which leads to azoospermia and testicular atrophy in animals during the chronic phase of the infection.The aim of this study was to reveal the kinetics of Leishmania chagasi infection in the genital system of male golden hamsters(Mesocricetus auratus).Methods:Animals were intraperitoneally inoculated with amastigotes from L.chagasi.At different time points animals were euthanized and genital organs processed for histo-pathological,qPCR,cytokines and testosterone detection assays.Results:Our results showed a high parasite load in testis,followed by an increase of pro-inflammatory cytokines IL1-β,TNF-αand IFN-γ,and testosterone.Subsequently,IL-4 expression was upregulated and basal parasite persistence in testis was observed using the experimental approach.Conclusion:Extracellular amastigotes migrated to the epididymis posing as a potential major factor of parasite persistence and venereal transmission of L.chagasi infection in hamsters.展开更多
基金supported by grants and fellowships from Fundação de AmparoàPesquisa do Estado de Minas Gerais,Conselho Nacional de Desenvolvimento Científico e Tecnológico,and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior.
文摘Background:Leishmaniasis causes alterations and lesions in the genital system,which leads to azoospermia and testicular atrophy in animals during the chronic phase of the infection.The aim of this study was to reveal the kinetics of Leishmania chagasi infection in the genital system of male golden hamsters(Mesocricetus auratus).Methods:Animals were intraperitoneally inoculated with amastigotes from L.chagasi.At different time points animals were euthanized and genital organs processed for histo-pathological,qPCR,cytokines and testosterone detection assays.Results:Our results showed a high parasite load in testis,followed by an increase of pro-inflammatory cytokines IL1-β,TNF-αand IFN-γ,and testosterone.Subsequently,IL-4 expression was upregulated and basal parasite persistence in testis was observed using the experimental approach.Conclusion:Extracellular amastigotes migrated to the epididymis posing as a potential major factor of parasite persistence and venereal transmission of L.chagasi infection in hamsters.
