Sound pollution(noise) is an increasing environmental concern,particularly associated with neurological and neurobehavioral abnormalities.However,the molecular mechanisms underlying noise-induced neural damage remain ...Sound pollution(noise) is an increasing environmental concern,particularly associated with neurological and neurobehavioral abnormalities.However,the molecular mechanisms underlying noise-induced neural damage remain unclear.In this study,we conducted transcriptional profiling of zebrafish to investigate the mechanisms underlying acoustic stimulation(1,000 Hz,130 d B).RNA sequencing and subsequent experiments revealed that TRPV1 is an important mediator of noise-induced neural damage in Hu C(elavl3)-GFP transgenic zebrafish.The results demonstrated that inhibiting TRPV1 significantly mitigated noise-induced neural damage in zebrafish with trpv1 gene RNAi and in mice with Trpv1 knockout(Trpv1~(-/-)).Specifically,TRPV1 antagonism significantly reduced neural damage in zebrafish and mice under noise exposure.Furthermore,activated TRPV1 could induce endoplasmic reticulum stress,leading to apoptosis and resulting in neural damage in mice and HEK293T cells.The findings of this study not only enhance our understanding of the molecular mechanisms underlying sound-induced neural damage but also highlight a novel target for drug intervention.展开更多
YpsR,a pivotal regulatory protein in the quorum-sensing(QS)of Yersinia pseudotuberculosis(Y.pstb),is essential for molecular signaling,yet its molecular mechanisms remain poorly understood.Herein,this study systemati-...YpsR,a pivotal regulatory protein in the quorum-sensing(QS)of Yersinia pseudotuberculosis(Y.pstb),is essential for molecular signaling,yet its molecular mechanisms remain poorly understood.Herein,this study systemati-cally investigates the interactions between YpsR and acyl-homoserine lactones(AHLs),shedding light on the selective mechanism of YpsR to various AHL molecules.Using molecular docking and surface plasmon resonance(SPR)analysis,we confirmed YpsR’s binding affinities,with the strongest observed for 3OC6-HSL,which notably inhibited Y.pstb growth.Additionally,we engineered a whole-cell biosensor based on YpsR-AHL interaction,which exhibited sensitivity to the signal molecule 3OC6-HSL produced by Y.pstb.Furthermore,key YpsR resi-dues(S32,Y50,W54,D67)involved in AHL binding were identified and validated.Overall,this research elu-cidates the mechanisms of QS signal recognition in Y.pstb,providing valuable insights that support the development of diagnostic tools for detecting Y.pstb infections.展开更多
Neuromorphic computing has the potential to achieve the requirements of the next-generation artificial intelligence(AI)systems,due to its advantages of adaptive learning and parallel computing.Meanwhile,biocomputing h...Neuromorphic computing has the potential to achieve the requirements of the next-generation artificial intelligence(AI)systems,due to its advantages of adaptive learning and parallel computing.Meanwhile,biocomputing has seen ongoing development with the rise of synthetic biology,becoming the driving force for new generation semiconductor synthetic biology(SemiSynBio)technologies.DNA-based biomolecules could potentially perform the functions of Boolean operators as logic gates and be used to construct artificial neural networks(ANNs),providing the possibility of executing neuromorphic computing at the molecular level.Herein,we briefly outline the principles of neuromorphic computing,describe the advances in DNA computing with a focus on synthetic neuromorphic computing,and summarize the major challenges and prospects for synthetic neuromorphic computing.We believe that constructing such synthetic neuromorphic circuits will be an important step toward realizing neuromorphic computing,which would be of widespread use in biocomputing,DNA storage,information security,and national defense.展开更多
基金supported by the National Natural Science Foundation of China (32270813)Young Scientists Fund of the National Natural Science Foundation of China (22305173)+2 种基金National Key R&D Program of China (2023YFC3605305)Beijing NOVA Program (20220484230)Youth Innovation Science Fund,PLA General Hospital (22QNCZ016)。
文摘Sound pollution(noise) is an increasing environmental concern,particularly associated with neurological and neurobehavioral abnormalities.However,the molecular mechanisms underlying noise-induced neural damage remain unclear.In this study,we conducted transcriptional profiling of zebrafish to investigate the mechanisms underlying acoustic stimulation(1,000 Hz,130 d B).RNA sequencing and subsequent experiments revealed that TRPV1 is an important mediator of noise-induced neural damage in Hu C(elavl3)-GFP transgenic zebrafish.The results demonstrated that inhibiting TRPV1 significantly mitigated noise-induced neural damage in zebrafish with trpv1 gene RNAi and in mice with Trpv1 knockout(Trpv1~(-/-)).Specifically,TRPV1 antagonism significantly reduced neural damage in zebrafish and mice under noise exposure.Furthermore,activated TRPV1 could induce endoplasmic reticulum stress,leading to apoptosis and resulting in neural damage in mice and HEK293T cells.The findings of this study not only enhance our understanding of the molecular mechanisms underlying sound-induced neural damage but also highlight a novel target for drug intervention.
基金the National Natural Science Foundation of China(32270813)Young Scientists Fund of the National Natural Science Foundation of China(22305173)Beijing Natural Science Foundation(5244033)for supporting this work.
文摘YpsR,a pivotal regulatory protein in the quorum-sensing(QS)of Yersinia pseudotuberculosis(Y.pstb),is essential for molecular signaling,yet its molecular mechanisms remain poorly understood.Herein,this study systemati-cally investigates the interactions between YpsR and acyl-homoserine lactones(AHLs),shedding light on the selective mechanism of YpsR to various AHL molecules.Using molecular docking and surface plasmon resonance(SPR)analysis,we confirmed YpsR’s binding affinities,with the strongest observed for 3OC6-HSL,which notably inhibited Y.pstb growth.Additionally,we engineered a whole-cell biosensor based on YpsR-AHL interaction,which exhibited sensitivity to the signal molecule 3OC6-HSL produced by Y.pstb.Furthermore,key YpsR resi-dues(S32,Y50,W54,D67)involved in AHL binding were identified and validated.Overall,this research elu-cidates the mechanisms of QS signal recognition in Y.pstb,providing valuable insights that support the development of diagnostic tools for detecting Y.pstb infections.
文摘Neuromorphic computing has the potential to achieve the requirements of the next-generation artificial intelligence(AI)systems,due to its advantages of adaptive learning and parallel computing.Meanwhile,biocomputing has seen ongoing development with the rise of synthetic biology,becoming the driving force for new generation semiconductor synthetic biology(SemiSynBio)technologies.DNA-based biomolecules could potentially perform the functions of Boolean operators as logic gates and be used to construct artificial neural networks(ANNs),providing the possibility of executing neuromorphic computing at the molecular level.Herein,we briefly outline the principles of neuromorphic computing,describe the advances in DNA computing with a focus on synthetic neuromorphic computing,and summarize the major challenges and prospects for synthetic neuromorphic computing.We believe that constructing such synthetic neuromorphic circuits will be an important step toward realizing neuromorphic computing,which would be of widespread use in biocomputing,DNA storage,information security,and national defense.
