[Objectives] The research aimed to optimize extraction process of Clerodendrum philippinum Schauer var. simplex Mlodenke total flavonoids( CPTF),and provide reference for its development and utilization. [Methods] Bas...[Objectives] The research aimed to optimize extraction process of Clerodendrum philippinum Schauer var. simplex Mlodenke total flavonoids( CPTF),and provide reference for its development and utilization. [Methods] Based on single-factor test,ethanol concentration,extraction temperature and extraction time were taken as independent variables,and total flavonoids yield was taken as dependent variable. The test was conducted according to central composite design principle. Multivariate linear regression and binomial equation fitting of the result were conducted,and extraction process of CPTF was optimized by using response surface methodology. [Results]The optimal extraction process of CPTF was as below: ethanol concentration 54. 76%,extraction temperature 83. 92℃,extraction time 102. 64 min,solid-liquid ratio 1:20,extraction for twice. [Conclusions] The extraction process of CPTF by central composite design-response surface methodology was simple and feasible,with reliable prediction result,which was suitable for industrial production.展开更多
[Objectives]To optimize extraction process of total flavonoids from Akebia trifoliata( Thunb.) Koidz.,so as to provide references for development and use of Akebia trifoliata( Thunb.) Koidz. [Methods]The extraction ra...[Objectives]To optimize extraction process of total flavonoids from Akebia trifoliata( Thunb.) Koidz.,so as to provide references for development and use of Akebia trifoliata( Thunb.) Koidz. [Methods]The extraction rate of total flavonoids of Akebia trifoliata( Thunb.)Koidz. was taken as observation indicator. On the basis of single factor experiment,central composite design( CCD) was used to evaluate the effects of the extraction temperature,extraction time,and ethanol concentration on the extraction process. Multiple linear regression and binomial fitting were used,and response surface methodology( RSM) was used to select the optimum extraction process. [Results] The optimum extraction process conditions for total flavonoids of Akebia trifoliata( Thunb.) Koidz. was extraction temperature: 83. 92 ℃; extraction time:96. 47 min; ethanol concentration: 63. 92%; extraction times: two times; solid to liquid ratio: 1 ∶ 20; extraction rate of total flavonoids:4. 55%. [Conclusions] The central composite design-response surface methodology( CCD-RSM) is simple,convenient,and feasible for extraction of total flavonoids from Akebia trifoliata( Thunb.) Koidz.,and the prediction results are reliable.展开更多
[Objectives] The aim was to study the protection effect of Qiwei Jingganling on carbon tetrachloride-induced acute liver injury in mice and its mechanism of action. [Methods]Total 60 mice were randomly and evenly divi...[Objectives] The aim was to study the protection effect of Qiwei Jingganling on carbon tetrachloride-induced acute liver injury in mice and its mechanism of action. [Methods]Total 60 mice were randomly and evenly divided into 6 groups,normal group,model group,silymarin group( 150 mg/kg),high-dose Qiwei Jingganling group( 8 g/kg,crude drug),middle-dose Qiwei Jingganling group( 4 g/kg,crude drug) and low-dose Qiwei Jingganling group( 2 g/kg,crude drug). The mice were administered orally once a day according to the amount of10 m L/kg,and 10-day continuous administration was carried out. After 2 h of the last administration,0. 12% CCl4 peanut oil solution( 10 m L/kg) was injected intraperitoneally to all the mice except those in the normal group to establish acute liver injury model. After 16 h,the blood of the mice was collected from the eyeballs,and their liver tissues were collected. The levels of alanine aminotransferase( ALT),aspartate aminotransferase( AST),superoxide dismutase( SOD),malondialdehyde( MDA) and glutathione peroxidase( GSH-Px) in the sera were determined by biochemical methods,and the contents of tumor necrosis factor-α( TNF-α),interleukin-1β( IL-1β) and interleukin-6( IL-6) in the liver tissues were determined with enzyme-linked immunosorbent assays( ELISA). [Results]Qiwei Jingganling significantly reduced the activities or content of ALT,AST and MDA in serum of mice with liver injury( P < 0. 05,P < 0. 01),increased the activities of SPD and GSH-Px( P < 0. 05,P < 0. 01) and down-regulated the expression levels of IL-1β,IL-6 and TNF-α in liver tissue( P < 0. 05,P < 0. 01).[Conclusions]Qiwei Jingganling has a good protection effect on CCl4-induced acute liver injury in mice,which may be related to the inhibition of oxidative stress and inhibition of inflammatory responses.展开更多
基金Supported by Special Item for Guangxi Bagui Scholars(Guicaijiaohan [2017]143)
文摘[Objectives] The research aimed to optimize extraction process of Clerodendrum philippinum Schauer var. simplex Mlodenke total flavonoids( CPTF),and provide reference for its development and utilization. [Methods] Based on single-factor test,ethanol concentration,extraction temperature and extraction time were taken as independent variables,and total flavonoids yield was taken as dependent variable. The test was conducted according to central composite design principle. Multivariate linear regression and binomial equation fitting of the result were conducted,and extraction process of CPTF was optimized by using response surface methodology. [Results]The optimal extraction process of CPTF was as below: ethanol concentration 54. 76%,extraction temperature 83. 92℃,extraction time 102. 64 min,solid-liquid ratio 1:20,extraction for twice. [Conclusions] The extraction process of CPTF by central composite design-response surface methodology was simple and feasible,with reliable prediction result,which was suitable for industrial production.
基金Supported by Key Scientific and Technological Planning Project of Guangxi(Gui Ke Neng 10100027-4)
文摘[Objectives]To optimize extraction process of total flavonoids from Akebia trifoliata( Thunb.) Koidz.,so as to provide references for development and use of Akebia trifoliata( Thunb.) Koidz. [Methods]The extraction rate of total flavonoids of Akebia trifoliata( Thunb.)Koidz. was taken as observation indicator. On the basis of single factor experiment,central composite design( CCD) was used to evaluate the effects of the extraction temperature,extraction time,and ethanol concentration on the extraction process. Multiple linear regression and binomial fitting were used,and response surface methodology( RSM) was used to select the optimum extraction process. [Results] The optimum extraction process conditions for total flavonoids of Akebia trifoliata( Thunb.) Koidz. was extraction temperature: 83. 92 ℃; extraction time:96. 47 min; ethanol concentration: 63. 92%; extraction times: two times; solid to liquid ratio: 1 ∶ 20; extraction rate of total flavonoids:4. 55%. [Conclusions] The central composite design-response surface methodology( CCD-RSM) is simple,convenient,and feasible for extraction of total flavonoids from Akebia trifoliata( Thunb.) Koidz.,and the prediction results are reliable.
基金Supported by"Bagui Scholars"Special Funds of Guangxi Zhuang Autonomous Region(GCJH[2017]143)
文摘[Objectives] The aim was to study the protection effect of Qiwei Jingganling on carbon tetrachloride-induced acute liver injury in mice and its mechanism of action. [Methods]Total 60 mice were randomly and evenly divided into 6 groups,normal group,model group,silymarin group( 150 mg/kg),high-dose Qiwei Jingganling group( 8 g/kg,crude drug),middle-dose Qiwei Jingganling group( 4 g/kg,crude drug) and low-dose Qiwei Jingganling group( 2 g/kg,crude drug). The mice were administered orally once a day according to the amount of10 m L/kg,and 10-day continuous administration was carried out. After 2 h of the last administration,0. 12% CCl4 peanut oil solution( 10 m L/kg) was injected intraperitoneally to all the mice except those in the normal group to establish acute liver injury model. After 16 h,the blood of the mice was collected from the eyeballs,and their liver tissues were collected. The levels of alanine aminotransferase( ALT),aspartate aminotransferase( AST),superoxide dismutase( SOD),malondialdehyde( MDA) and glutathione peroxidase( GSH-Px) in the sera were determined by biochemical methods,and the contents of tumor necrosis factor-α( TNF-α),interleukin-1β( IL-1β) and interleukin-6( IL-6) in the liver tissues were determined with enzyme-linked immunosorbent assays( ELISA). [Results]Qiwei Jingganling significantly reduced the activities or content of ALT,AST and MDA in serum of mice with liver injury( P < 0. 05,P < 0. 01),increased the activities of SPD and GSH-Px( P < 0. 05,P < 0. 01) and down-regulated the expression levels of IL-1β,IL-6 and TNF-α in liver tissue( P < 0. 05,P < 0. 01).[Conclusions]Qiwei Jingganling has a good protection effect on CCl4-induced acute liver injury in mice,which may be related to the inhibition of oxidative stress and inhibition of inflammatory responses.
